DOI: 10.3724/SP.J.1145.2013.00941

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.941-944

DNA-protein Crosslinks and Micronuclei Induced by Chronic Nitrogen Dioxide Inhalation in Rats

Nitrogen dioxide (NO2) is a ubiquitous reactive free-radical gas associated with momentary and chronic health effects. In the present study, chronic NO2 inhalation-induced effects on the DNA-protein crosslinks (DPC) and micronuclei (MN) in rats were investigated, by exposing Wistar rats to NO2 at 0.25 and 5 mg m-3. The results showed that chronic inhalation exposure of rats to NO2 not only induced the formation of DPC in cells of various internal organs (the brain, lungs, liver, spleen, kidneys and heart), but also resulted in obvious increase of MN frequency in the bone marrow cells of rats. Furthermore, the above responses showed signi fi cant linear dose-dependent manners. These results implicated that NO2 is a clastogenic and genotoxic agent, which may be an important reason for health problem induced by chronic inhalation exposure to NO2. Fig 1, Tab 1, Ref 22

Key words:nitrogen dioxide,rat,DNA-protein crosslinks,micronucleus

ReleaseDate:2015-04-15 08:42:19

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