doi:

DOI: 10.3724/SP.J.1145.2013.00969

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.969-973

Improved Drought Tolerance of Transgenic Arabidopsis thaliana by Inducible Expression of XERICO Gene


Abstract:
To develop drought-resistant plants or crops, we constructed the PSARK::XERICO plant expression vector and transformed it into the model plant Arabidopsis thaliana (Col-0 ecotype) to analyze the physiological phenotypes. In this study, SARK promoter was isolated from recombinant plasmid of pSARK-IPT; full length of XERICO gene was ampli fi ed from the cDNA of Arabidopsis seedlings by the RT-PCR technique; the SARK promoter and the XERICO gene replaced CaMV 35S promoter and GUS gene respectively to obtain the recombinant plasmid, designated as pBI121-pSARK-XERICO . The constructed expression vector was transformed into the wild-type Arabidopsis through the fl oral dip method. The homozygous lines were obtained through selection of antibiotic resistance and analysis of genetic segregation. The obtained homozygous seedlings were assayed by PCR and evaluated through abscisic acid (ABA) sensitivity analysis during seed germination, and analyzed through the results of phenotypic observation after salinity and mannitol stress treatment during early seedling growth. In addition, the analysis of drought tolerance was conducted between the transgenic PSARK::XERICO and wild-type Arabidopsis. Compared with the wild-type and xerico mutant plants, the transgenic PSARK::XERICO plants were more sensitive to 1.0 μmol/L ABA, 100 mmol/L NaCl and 200 mmol/L mannitol. The expression of water de fi cit-inducible XERICO gene in Arabidopsis enhanced the tolerance to drought stress. Therefore, this study indicated that PSARK::XERICO has the potential for breeding drought-tolerant plants or crops. Fig 4, Tab 1, Ref 19

Key words:Arabidopsis thaliana,SARK promoter,XERICO gene,ABA,drought tolerance

ReleaseDate:2015-04-15 08:42:21



[1] Mittler R, Blumwald E. Genetic engineering for modern agriculture:challenges and perspectives [J]. Annu Rev Plant Biol, 2010, 61: 443-462

[2] Pasapula V, Shen GX, Kuppu S, Paez-Valencia J, Mendoza M, HouP, Chen J, Qiu XY, Zhu LF, Zhang XL, Auld D, Blumwald E, ZhangH, Gaxiola R, Payton P. Expression of an Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1 ) in cotton improves drought- and salttolerance and increases fibre yield in the field conditions [J]. PlantBiotech J, 2011, 9: 88-99

[3] 杨帆, 苗灵凤, 胥晓, 李春阳 . 植物对干旱胁迫的响应研究进展[J]. 应用与环境生物学报, 2007, 13 (4): 586-591 [Yang F, Miao LF, Xu X, LiCY. Progress in research of plant responses to drought stress [J]. Chin JAppl Environ Biol, 2007, 13 (4): 586-591]

[4] 宁 约 瑟, 王 国 梁, 谢 旗 . 泛 素 连 接 酶 E3 介 导 的 植 物 干旱 胁 迫 反应[J]. 植物学报, 2011, 46 (6): 606-616 [Ning YS, Wang GL, Xie Q. E3Ubiquitin ligase-mediated drought responses in plants [J]. Chin Bull Bot, 2011, 46 (6): 606-616]

[5] Peleg Z, Blumwald E. Hormone balance and abiotic stress tolerance incrop plants [J]. Curr Opin Plant Biol, 2011, 14 (3): 290-295

[6] Boyer JS. Plant productivity and environment [J ]. Science, 1982, 218(4571): 443-448

[7] Peleg Z, Apse MP, Blumwald E. Engineering salinity and water-stresstolerance in crop plants: getting closer to the fi eld [J]. Adv Bot Res, 2011, 57: 405-443

[8] Rivero RM, Kojima M, Gepstein A, Sakakibara H, Mittler R, Gepstein S, Blumwald E. Delayed leaf senescence induces extreme drought tolerancein a fl owering plant [J]. Proc Natl Acad Sci USA, 2007, 104 (49): 19631-19636

[9] R ive ro R M, Shu la ev V, Blu mwald E. C y t ok i n i n- de pe nde ntphotorespiration and the protection of photosynthesis during water de fi cit[J]. Plant Physiol, 2009, 150 (3): 1530-1540

[10] Rivero RM, Gimeno J, Van Deynze A, Walia H, Blumwald E. Enhancedcytokinin synthesis in tobacco plants expressing P SARK::IPT prevents thedegradation of photosynthetic protein complexes during drought [J].Plant Cell Physiol, 2010, 51 (11): 1929-1941

[11] Peleg Z, Reguera M, Tumimbang E, Walia H, Blumwald E. Cytokininmediated source/sink modifications improve drought tolerance andincreases grain yield in rice under water stress [J]. Plant Biotech J, 2011, 9 (7): 747-758

[12] Qin H, Gu Q, Zhang J, Sun L, Kuppu S, Zhang Y, Burow M, Payton P, Blumwald E, Zhang H. Regulated expression of anisopentenyltransferase gene (IPT ) in peanut significantly improvesdrought tolerance and increases yield under fi eld conditions [J]. PlantCell Physiol, 2011, 52 (11): 1904-1914

[13] Kuppu S, Mishra N, Hu R, Sun L, Zhu X, Shen G, Blumwald E, Payton P, Zhang H. Water-de fi cit inducible expression of a cytokinin biosyntheticgene IPT improves drought tolerance in cotton [J]. PLoS ONE, 2013, 8(5): e64190

[14] Ko J, Yang SH, Han K. Upregulation of an Arabidopsis RING-H2 gene, XERICO, confers drought tolerance through increased abscisic acidbiosynthesis [J]. Plant J, 2006, 47 (3): 343-355

[15] Zentella R, Zhang Z, Park M, Thomas SG, Endo A, Murase K, FleetCM, Jikumaru Y, Nambara E, Kamiya Y, Sun T. Global analysis ofDELLA direct targets in early gibberellin signaling in Arabidopsis [J].Plant Cell, 2007, 19 (10): 3037-3057

[16] Murashige T, Skoog F. A revised medium for rapid growth and bioassayswith tobacco tissue cultures [J]. Physiol Plant, 1962, 15 (3): 473-497

[17] Clough SJ, Bent AF. Floral dip: a simpli fi ed method for Agrobacteriummediated transformation of Arabidopsis thaliana [J]. Plant J, 1998, 16(6): 735-743

[18] Delatorrea CA, Cohena Y, Liu L, Pelega Z, Blumwald E. The regulationof the SARK promoter activity by hormones and environmental signals[J]. Plant Sci, 2012, 193-194: 39-47

[19] Eckardt NA. GA signaling: direct targets of DELLA proteins [J]. PlantCell, 2007, 19 (10): 2970