DOI: 10.3724/SP.J.1145.2013.00969

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.969-973

Improved Drought Tolerance of Transgenic Arabidopsis thaliana by Inducible Expression of XERICO Gene

To develop drought-resistant plants or crops, we constructed the PSARK::XERICO plant expression vector and transformed it into the model plant Arabidopsis thaliana (Col-0 ecotype) to analyze the physiological phenotypes. In this study, SARK promoter was isolated from recombinant plasmid of pSARK-IPT; full length of XERICO gene was ampli fi ed from the cDNA of Arabidopsis seedlings by the RT-PCR technique; the SARK promoter and the XERICO gene replaced CaMV 35S promoter and GUS gene respectively to obtain the recombinant plasmid, designated as pBI121-pSARK-XERICO . The constructed expression vector was transformed into the wild-type Arabidopsis through the fl oral dip method. The homozygous lines were obtained through selection of antibiotic resistance and analysis of genetic segregation. The obtained homozygous seedlings were assayed by PCR and evaluated through abscisic acid (ABA) sensitivity analysis during seed germination, and analyzed through the results of phenotypic observation after salinity and mannitol stress treatment during early seedling growth. In addition, the analysis of drought tolerance was conducted between the transgenic PSARK::XERICO and wild-type Arabidopsis. Compared with the wild-type and xerico mutant plants, the transgenic PSARK::XERICO plants were more sensitive to 1.0 μmol/L ABA, 100 mmol/L NaCl and 200 mmol/L mannitol. The expression of water de fi cit-inducible XERICO gene in Arabidopsis enhanced the tolerance to drought stress. Therefore, this study indicated that PSARK::XERICO has the potential for breeding drought-tolerant plants or crops. Fig 4, Tab 1, Ref 19

Key words:Arabidopsis thaliana,SARK promoter,XERICO gene,ABA,drought tolerance

ReleaseDate:2015-04-15 08:42:21

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