doi:

DOI: 10.3724/SP.J.1145.2013.00980

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.980-985

Influence of Xanthomonas oryzae pv. oryzae PX099 Inoculation on Antioxidant Enzyme Activity and Defense Gene Expression in CBB23


Abstract:
In order to study the variation of bacterial blight resistance related materials in rice upon pathogen induction, seedlings of bacterial blight resistant near-isogenic line CBB23 and its susceptible recurrent parent JG30 were inoculated with Xanthomonas oryzae pv. oryzae strain PX099. The in fl uence of pathogen induction on the antioxidant enzyme activities was measured and the expression of some defense genes analyzed by RT-PCR. The results showed that the PX099 inoculation fi rst increased then decreased the SOD, POD and CAT activities of CBB23, with the degree of increase greater than that in JG30; MDA contents were also increased in CBB23 with the degree of increase lower than that in JG30; CBB23 showed higher expression of defense genes than JG30, including Oryza sativa pathogenesis-related gene (OsPR1 ), Oryza sativa endochitinase gene (OsPR4) and Oryza sativa defense-related transcription factor control gene (OsMyb). In general, the disease resistance ability of CBB23 to PX099 is signi fi cantly stronger than that of susceptible cultivar JG30, which is conductive to the application of CBB23. Fig 4, Tab 2, Ref 34

Key words:rice bacterial blight,defense gene,antioxidant enzyme,CBB23

ReleaseDate:2015-04-15 08:42:21



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