doi:

DOI: 10.3724/SP.J.1145.2013.00990

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.990-996

Expression of Endoglucanase Gene and β-Glucosidase Genes in Bacillus subtilis


Abstract:
In order to archive the secretory coexpression of cellulase genes, this study used the pP43JM2 shuttle vector carrying NprB signal peptide of the Bacillus subtilis neutral protease as expression vector to express in B. subtilis WB800 the celA gene from Clostridium thermocellum DSM 1237 encoding endoglucanase and bglA and bglB genes from B. polymyxa encoding β-glucosidases. The results showed that the endoglucanase encoded by celA and the β-glucosidases encoded by bglB were secreted into the culture broth successfully; the two cellulase genes could be coexpressed and secreted; the extracellular enzyme solution reacted with PASC and cellobiose, producing 674 and 24 mg/L glucose, respectively; on the contrary, the β-glucosidases encoded by bglA did not react with PASC, only with cellobiose, its intracellular enzyme solution producing 938.7 mg/L glucose. The current work suggested a possible method for multiple cellulase secretion in B. subtilis and may provide a way of economical construction of bioprocessing strains for bulk chemical production. Fig 6, Tab 2, Ref 18

Key words:Bacillus subtilis,β-glucosidase,endoglucanase,extracellular secretion,coexpression

ReleaseDate:2015-04-15 08:42:22



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