DOI: 10.3724/SP.J.1145.2013.01020

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.1020-1024

PCR-DGGE Analysis of Lactobacillus Diversity in Pit Muds of Different Cellar-aged Luzhou Laojiao Liquor

Lactobacillus is an important functional bacterium in pit mud, participating in the formation of strong aromatic liquor. Therefore exploring lactic acid bacteria species in pit mud is of great signif icance for the improvement of liquor quality. In this study, seven cellar pit mud samples were collected from different-aged cellars in Luzhou Laojiao Liquor Co. Ltd. The DNA was isolated, and the diversity of lactic acid bacteria was analyzed by PCR-DGGE technique. The results of DGGE showed signif icant diversity of lactic acid bacteria in the pit muds. Analysis of the diversity index showed the Patrick richness index R between 14 and 19; Shannon-Wiener index H varied between 2.634 5 and 2.888 9; and Simpson index C was between 0.055 7 and 0.055 7. Furthermore, 16 representative DGGE bands were picked, rinsed, and then amplifi ed by using primers LAC1/LAC2, the PCR fragments were cloned and sequenced. The results revealed that the lactic acid bacteria in differentaged cellar pit mud samples were very abundant, belonging to genus Lactobacillus, Pediococcus, Weissella, Enterococcus, Exiguobacterium, Bacillus, Rummeliibacillus, and Cohnella, respectively. The predominant lactic acid bacteria among all the pit muds were Lactobacillus acetotolerans. Fig 5, Tab 2, Ref 24

Key words:cellar pit mud,PCR-DGGE,Luzhou- flavor,lactic acid bacteria,diversity

ReleaseDate:2015-04-15 08:42:22

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