doi:

DOI: 10.3724/SP.J.1145.2013.01053

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.1053-1057

Effect of Different Additives on the Quality of Corn Stover Silage


Abstract:
In order to study the effects of different additives on the quality of corn stover silage, the corn stover was cut and then ensiled with a certain ratio of lactobacillus and yeast(A), the complex inoculants(B), cellulase(C), urea(D) and corn f lour(E) by using orthogonal experiment design. Based on the evaluation system of sensory properties and silage fermentation quality, the optimal combination of the corn straw silage additives was found to be A3B3C3D1E1, namely, a ratio of 1 : 0 for lactobacillus and yeast, the complex inoculants 0.6%, cellulase 1.0 g/kg, urea 0.3% and corn meal 0%. The analysis of sensory properties, organic acids and microbe number of silage showed a signif icant inf luence of complex inoculant dosage on sensory properties, as well as signifi cant effects of the dosage of cellulase and complex inoculants on lactic acid content(P 0.05). However, none of the factors showed signif icant effect on acetic acid content, yeast or mold number, indicating that the addition of complex inoculants and cellulase promotes the lactic acid bacteria breeding, while the addition of corn f lour and urea has less effect on quality of corn straw silage. Tab 5, Ref 25

Key words:silage inoculants,cellulase,urea,corn flour,quality evaluation,corn stover,orthogonal experiment

ReleaseDate:2015-04-15 08:42:24



[1] 席兴军, 韩鲁佳, 原慎一郎, 野中和久. 添加乳酸菌和纤维素酶对玉米 秸秆青贮饲料品质的影响 [J]. 中国农业大学学报, 2003, 8 (2): 21-24[Xi XJ, Han RJ, Hara S, Nanaka K. Effects of lactobacillus and cellulase on the quality of corn stover silage [J]. J China AgricUniv, 2003, 8 (2):21-24]

[2] 季昀, 潘晓花, 王龙, 蔡晶晶. 玉米青贮添加剂研究进展[J]. 广东饲料, 2011, 20 (2): 27-29

[3] Acosta Arag’on Y, Jatkauskas J, Vrotniakien˙e V. The effect of a silage inoculant on silage quality, aerobic stability, and meat production on farm scale [J]. ISRN Veter Sci, 2012, 2012, Article ID 345927

[4] Jonas L. Effect of inoculation on silage quality and rumen fermentation in dairy cows [J]. Medycyna Wet, 2007, 63 (9): 1057-1060

[5] Stokes MR. Effects of an enzyme mixture, an inoculant, and their interaction on silage fermentation and dairy production [J]. J Dairy Sci, 1992, 75: 764-773

[6] Jacobs JL, McAllan AB. Enzymes as sila ge additives. 1. Silage quality, digestion, digestibility and performance in growing cattle [J]. Grass For Sci, 1991, 46 (11): 63-73

[7] 臧艳运, 王雁, 顾学莹, 陈鹏飞, 杨富裕. 添加丙酸和尿素对玉米青贮 品质的影响 [J]. 草业学报, 2012, 29 (1): 156-159 [Zang YY, Wang Y, Gu XY, Chen PF, Yang FY. Effect of propionic acid and urea on the quality of whole-crop corn silage [J]. Pratacult Sci, 2012, 29 (1): 156-159]

[8] Lei XG, Stahl CH. Biotechnological development of effective phytases for mineral nutrition and environmental protection [J]. Appl Microbiol Biotechnol, 2001, 57 (4) : 474- 481

[9] 玉柱, 孙启忠, 于艳冬, 王美容. 添加尿素和乳酸菌制剂对玉米秸秆青 贮料品质的影响 [J]. 中国畜牧杂志, 2009, 45 (3): 37-39 [Yu Z, Sun QZ, Yu YD, Wang MR. Effects of urea and lactic bacteria on the quality of corn stalk silages [J]. Chin J Anim Sci, 2009, 45 (3): 37-39]

[10] 梁瑜, 雷赵民, 吴建平, 刘婷. 不同添加剂(物)对玉米秸秆青贮有 机酸含量的影响 [J]. 甘肃农业大学学报, 2012, 47 (5): 34-39 [Liang Y, Lei ZM, Wu JP, Liu T. In fl uence of different additives on the organic acid of corn silage [J]. J Gansu Agric Univ, 2012, 47 (5): 34-39]

[11] 王静. 玉米秸整秆打捆青贮工艺及品质的试验研究[D]. 杨凌: 西北 农林科技大学, 2010 [Wang J. The study of main factors and qualities on compressed silage process [D]. Yangling: Northw est A&F University, 2010]

[12] Seale DR. Bacterial inoculants as silage additives [J]. J Appl Bacteriol, 1986, 61 (15): 9-26

[13] 姜芳, 王佳堃, 刘建新. 内标法同时测定挥发性脂肪酸和乳酸的方 法探究[J]. 饲料检测, 2009, 45 (21): 73-76 [Jiang F, Wang JK, Li u JX. Study on simultaneous determination of VFAS and lactic acid by using internal standard method. Feed Testing, 2009, 45 (21): 73-76]

[14] 杨云贵, 张越利, 杜欣, 刘桂要, 曹社会. 2种玉米青贮饲料青贮过程 中主要微生物的变化规律研究[J]. 畜牧兽医学报, 2012, 43 (3): 397-403 [Yang YG, Zhang YL, Du X, Liu GY, Cao SH. Study on the major microorganism changes during the silage processing of two kinds of corn silage [J]. Acta Veter Zootech Sin, 2012, 43 (3): 397-403]

[15] Fleig. Futterb gauffutterbereit [J]. 1938 (2): 1-8

[16] 王国仓, 李增辉, 范秀兰. 微生物在青贮饲料中的作用 [J]. 内蒙古畜 牧科学, 2003 (3): 55-56

[17] 白晓婷. 酵母类产品在饲料中的研究与应用 [J]. 中国饲料, 2005 (2):8-10 [Bai XT. Study and application of yeast products in animal feeding[J]. Chin Feeding, 2005 (2): 8-10]

[18] 任付平. 复合微生物菌剂在全株玉米青贮中的应用与研究[D]. 西北 大学, 2007 [Ren FP. Research and application of compound microbial additives on the whole-crop corn silage [D]. Xian: Northwest University, 2007]

[19] 司丙文, 王宗礼, 孙启忠, 张慧杰, 李峰. 尖叶胡枝子青贮微生物数 量变化及发酵特性[J]. 草叶科学, 2012 (4): 650-657 [Si BW, Wang ZL, Sun QZ, Zhang HJ, Li F. Fermentation characteristics changes of lactic acid bacteria isolated from ensiling Lespedeza hedysaroides [J]. PratacultSci, 2012 (4): 650-657]

[20] 余汝华, 莫放, 赵丽华, 张晓明, 陈瑶. 不同玉米品种青贮饲料营养成 分比较分析[J]. 中国农学通报, 2007, 23 (8): 17-20 [Yu RH, Mo F, Zhao LH, Zhang XM, Chen Y. The comparison of the chemical characteristics and nutritive value of green corn stalk silage with different variety [J]. Chin Agric Sci Bull, 2007, 23 (8): 17-20]

[21] 傅彤, 刘庆生, 范志影, 刁其玉. 应用离子色谱测定青贮饲料中有机 酸含量的研究[J]. 中国畜牧兽医, 2005, 32 (5): 16-17

[22] 穆怀彬. 近红外光谱技术在玉米营养品质和青贮玉米品质评定的 研究[D]. 呼和浩特: 内蒙古农业大学, 2008 [Mu HB. Applications of near infrared re fl ectance spectroscopy (NIRS) in the analysis of maize nutritional qualities and silage qualities [D]. Hohhot: Inner Mongolia Agricultural University, 2008]

[23] 刘建新, 杨振海, 叶均安, 史占全, 吴跃明 . 青贮饲料的合理调制与质 量评定标准[J]. 饲料工业, 1999, 20 (4): 3-5

[24] 王晓娜, 孙启忠, 韩海波, 张晓庆, 孙长乐 . 内蒙古青贮饲料质量研 究[J]. 草业学报, 2011, 20 (3): 149-155 [Wang XN, Sun QZ, Han HB, Zhang XQ, Sun CL. The quality of silage in Inner Mongolia [J]. Acta pratacult Sin, 2011, 20 (3): 149-155]

[25] 兴丽, 韩鲁佳, 刘贤, 蔡义民. 乳酸菌和纤维素酶对全株玉米青贮发 酵品质和微生物菌落的影响 [J]. 中国农业大学学报, 2004, 9 (5): 38-41 [Xing L, Ha RJ, Liu X, Cai YM. Effects of lactobacillus and cellulase on the fermentation characteristics and microorganism of whole-plant corn silage [J]. J China Agric Univ, 2004, 9 (5): 38-41]