DOI: 10.3724/SP.J.1145.2013.01058

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.1058-1063

Culture Optimization of Lipid Fermentation by Trichosporon cutaneum Using Corncob Residue

Corncob residue, solid waste after the xylose production from hemicellulose with high cellulose content, can be directly hydrolyzed by cellulase to produce microbial lipid. To produce microbial lipid eff iciently, f ive oleaginous yeasts were tested to screen the most optimal strain for lipid fermentation in the corncob residue hydrolysate. Then the nutrients in the hydrolysate were optimized and fi nally the lipid production was improved by increasing sugar concentration of the hydrolysate through changing cellulase amount. Trichosporon cutaneum 20271 could grow and produce microbial lipid in the corncob residue hydrolysate; and corn steep liquor(CSL) could signif icantly improve the lipid production. In addition, different nutrient did not influence the fatty acid composition. At last, the highest dry cell weight(DCW), lipid titer, lipid content and lipid productivity of 38.4 g/L, 12.3 g/L, 32.1% and 0.064 g L-1 h-1 were obtained respectively when cellulase dose was 15 FPU/g DM. We also found that T. cutaneum 20271 could produce microbial lipid with corncob residue hydrolydate, and that the lipid fermentation performance was improved by optimizing the hydrolysate medium. This study provided not only an economical feedstock option to produce microbial lipid, but also a practical method for CCR utilization. Fig 3, Tab 6, Ref 24

Key words:corncob residue,microbial lipid,Trichosporon cutaneum,medium optimization

ReleaseDate:2015-04-15 08:42:24

[1] Tyson KS. Biodi esel research progress 1992-1997 [R]. NREL Technical Report, 1998, NREL/TP-580-24443

[2] 赵宗保. 加快微生物油脂研究为生物柴油产业提供廉价原料[J]. 生 物工程杂志, 2005, 25 (2): 8-11 [Zhao ZB. Toward cheaper microbial oil for biodiesel oil [J]. China Biotechnol, 2005, 25 (2): 8-11]

[3] Gerpen JV. Business management for biodiesel producers [R]. NREL Technical Report, 2004, NREL/SR-510-36342

[4] Ratledge C. Microorganisms for lipids [J]. Acta Biotechnol, 1991, 11:429-438

[5] Chen X, Li ZH, Zhang XX, Hu FX, Ryu DDY, Bao J. Screening of oleaginous yeast strains tolerant to lignocellulose degradation compounds [J]. Appl Biochem Biotech, 2009, 159: 591-604

[6] Liu W, Wang YM, Yu ZC, Bao J. Simultaneous sacchari fication and microbial lipid fermentation of corn stover by oleaginous yeast Trichosporon cutaneum [J]. Bioresour Technol, 2012, 118: 13-18

[7] Yousuf A. Biodiesel from lignocellulosic biomass-prospects and challenges [J]. Waste Manage, 2012, 32: 2061-2067

[8] Huang X, Wang YM., Liu W, Bao J. Biological removal of inhibitors leads to the improved lipid production in the lipid fermentation of corn stover hydrolysate by Trichosporon cutaneum [J]. Bioresour Technol, 2011, 102: 9705-9709

[9] Liu K, Lin XH, Yue J, Li XZ, Fang X, Zhu MT, Lin JQ, Qu YB, Xiao L. High concentration ethanol production from corncob residues by fedbatch strategy [J]. Bioresour Technol, 2010, 101: 4952-4958

[10] Xiao X, Hou YY, Du J, Liu Y, Liu YJ, Dong LY, Liang QL, Wang YM, Bai G, Luo GA. Determination of main categories of c omponents in corn steep liquor by near-infrared spectroscopy and partial least-squares regression [J]. J Agric Food Chem, 2012, 60: 7830-7835

[11] 李 永 红, 刘波, 赵 宗 保, 白凤 武 . 圆红冬 孢 酵 母菌发 酵产油脂 培 养基及发酵条件的优化研究 [J]. 生物工程学报, 2006, 22(4): 650-656 [Li YH, Liu B, Zhao ZB, Bai FW. Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides [J]. Chin J Biotech, 2006, 22 (4): 650-656]

[12] Folch J, Lees M, Sloane-Stanley GH. A simple method for the isolation and puri fication of total lipids from animal tissues [J]. J Biol Chem, 1957, 226: 497-509

[13] D’Elia C, Steudler P, Corwin N. Determination of total nitrogen in aqueous samples using persulfate digestion [J]. Limnol Oceanogr, 1977, 22: 760-764

[14] Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent[J]. Nat Protoc, 2007, 2 (4): 875-877

[15] J?nsson LJ, Alriksson J, Nilvebrant NO. Bioconversion of lignocellulose: inhibitors and detoxi fi cation [J]. Biotechnol Biofuels, 2013, 6: 16

[16] Obroi HS, Vadlani PV, Brijwani K, Bhargav VK, Patil RT. Enhanced ethanol production via fermentation of rice straw with hydrolysateadapted Candida tropicalis ATCC 13803 [J]. Process Biochem, 2010, 45: 1299-1306

[17] Ratledge C, Wynn JP. The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms [J]. Adv Appl Microbiol, 2002, 51: 1-51

[18] 袁锦云, 艾佐佐, 张志斌, 颜日明, 曾庆桂, 朱笃. 皮状丝孢酵母 B3 利用木 薯淀粉发酵生 产微 生物油脂 [J]. 生物工程学报, 2011, 27 (3): 453-460 [Yuan JY, Ai ZZ, Zhang ZB, Yan RM, Zeng QG, Zhu D. Microbial oil production by Trichosporon cutaneum B3 using cassava starch [J]. Chin J Biotech, 2011, 27 (3): 453-460]

[19] Buurman ET, Teixeira de Mattos MJ, Neijssel OM. Futile cycling of ammonium ions via the high affi nity potassium uptake system (Kdp) of Escherichia coli [J]. Arch Microbiol, 1991, 155 (4): 391-395

[20] Zheng YB, Chi ZY, Birgitte K A, Chen SL. Oleaginous yeast Cryptococcus curvatus for biofuel production: ammonia’s effect [J]. Biomass Bioenergy, 2012, 37: 114-121

[21] 罗文, 袁振 宏, 谭天伟, 吕鹏梅 . 生物柴油理化性质与组分关 系的 研究[J]. 太阳能学报, 2008, 29 (7): 878-882 [Luo W, Yuan ZH, Tan TW, Lv PM. Study on the relationship between the fuel properties and components of biodiesel [J]. Acta Energ Sol Sin, 2008, 29 (7): 878-882]

[22] Tsigie YA, Wang CY, Truong CT, Ju YH. Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate [J]. Bioresour Technol, 2011, 102: 9216-9222

[23] 李园园, 吴虹, 黄超, 宗敏华. 发酵性丝孢酵母 HWZ004 利用水稻 秸秆水解液发酵产油脂 [J]. 生物工程学报, 2011, 27 (9): 1309-1316[Li YY, Wu H, Huang C, Zong MH. Using rice straw hydrolysate for microbial oil production by Trichosporon fermentans HWZ004 [J]. Chin J Biotech, 2011, 27 (9): 1309-1316]

[24] Huang C, Chen XF, Xiong L, Chen XD, Ma LL. Oil production by the yeast Trichosporon dermatis cultured in enzymatic hydrolysates of corncobs [J]. Bioresour Technol, 2012, 110: 711-714