doi:

DOI: 10.3724/SP.J.1145.2013.01058

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.1058-1063

Culture Optimization of Lipid Fermentation by Trichosporon cutaneum Using Corncob Residue


Abstract:
Corncob residue, solid waste after the xylose production from hemicellulose with high cellulose content, can be directly hydrolyzed by cellulase to produce microbial lipid. To produce microbial lipid eff iciently, f ive oleaginous yeasts were tested to screen the most optimal strain for lipid fermentation in the corncob residue hydrolysate. Then the nutrients in the hydrolysate were optimized and fi nally the lipid production was improved by increasing sugar concentration of the hydrolysate through changing cellulase amount. Trichosporon cutaneum 20271 could grow and produce microbial lipid in the corncob residue hydrolysate; and corn steep liquor(CSL) could signif icantly improve the lipid production. In addition, different nutrient did not influence the fatty acid composition. At last, the highest dry cell weight(DCW), lipid titer, lipid content and lipid productivity of 38.4 g/L, 12.3 g/L, 32.1% and 0.064 g L-1 h-1 were obtained respectively when cellulase dose was 15 FPU/g DM. We also found that T. cutaneum 20271 could produce microbial lipid with corncob residue hydrolydate, and that the lipid fermentation performance was improved by optimizing the hydrolysate medium. This study provided not only an economical feedstock option to produce microbial lipid, but also a practical method for CCR utilization. Fig 3, Tab 6, Ref 24

Key words:corncob residue,microbial lipid,Trichosporon cutaneum,medium optimization

ReleaseDate:2015-04-15 08:42:24



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