doi:

DOI: 10.3724/SP.J.1145.2013.01069

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:6 PP.1069-1072

Colorimetric Determination of Monosodium Glutamate in Food Samples Using Colorimetric Determination of Monosodium Glutamate in Food Samples Using L-glutamate Oxidase-glutamate Oxidase


Abstract:
A rapid, sensitive and simple chromogenic method was developed for quantitative determination of monosodium glutamate(MSG) in food samples. The method incorporated a glutamate oxidase and peroxidase. The librated H 2 O 2 from a glutamate sample as a result of enzymatic action was measured using 4-aminoantipyrine and phenol as a chromogenic reagent at 502 nm. Glutamate calibration curve was linear up to 125 mmol L-1 with a detection limit of 2 mmol L-1. The results of analyzing L-glutamate in various food samples using the approach were compared to those of a standard procedure employing HPLC method. MSG concentration in different samples ranged from 0.93 to 4.9 g/kg. This method is relatively sensitive and specifi c, without the need of pre-treatment for sample. Fig 5, Tab 1, Ref 25

Key words:MSG,colorimetry,L-glutamate oxidase,soup,noodle,hamburger

ReleaseDate:2015-04-15 08:42:24



[1] WHO. Technical Report Food Additives [R]. 1987, 22: 97-161

[2] Yang WH, Drouin MA, Herbert M, Mao Y, Karsh J. The monosodium glutamate symptom complex: assessment in a double-blind, placebocontrolled, randomized study [J]. J Allergy Clin Immunol, 1997, 99: 757

[3] Blaylock RL. Excitotoxins the Taste That Kills [M]. Santa Fe, New Mexico: Health Press, 1997

[4] Bader JM. La roulette russe du restaurant chinois [J]. J Assoc Offi cial Anal Chem Intern, Sci & Vie, 1992, 899: 58-65

[5] Meldrum BS. Glutamate as a neurotransmitter in the brain: review of physiology and pathology [J]. J Nutrition, 2000, 130S: 1007-1015

[6] Winter R. A Consumer’s Dictionary of Food Additives [M]. N.Y.: Crown publisher’s Inc., 1994. 1240

[7] A nd e r s on LW, Z a hor ev it z DW, St r ong J M. Glut a m i ne a nd glutamate: automated quantification and isotopic enrichments by gas chromatography/mass spectrometry [J]. Anal Biochem, 1997, 163: 358-368

[8] Wei H, Li SFY. Rugged gap reactor device for postcolumn fl uorescence detection in capillary electrophoresis [J]. Anal Chem, 1998, 70 (23): 5097-5102

[9] Lateef M, Siddiqui K, Saleem M, Iqbal L. Estimation of monosodium glutamate by modi fi ed HPLC method in various Pakistani spices formula[J]. J Chem Soc Pak, 2012, 34 (1): 39-24

[10] Shi R, Stein K. Flow injection methods for determination of L-glutamate using glutamate decarboxylase and glutamate dehydrogenase reactors with spectrophotometric detection [J]. Analyst, 1996, 121 (9): 1305-1309

[11] Liu Z, Niwa O, Horiuchi T, Kurita R, Torimitsu K. NADH and glutamate on-line sensors using Os-HRP/GC electrodes modi fi ed with NADH oxidase and glutamate dehydrogenase [J]. Biosens Bioelectron, 1999, 14 (7): 631-638.

[12] Ling D, Wu G, Wang CH, Wang F, Song G. The preparation and characterization of an immobilized L -glutamic decarboxylase and its application for determination of L -glutamic acid [J]. Enzyme Microb Technol, 2000, 27 (7): 516-521

[13] Bohmer A, Muller A, Passarge M, Liebs PHH, Muller HG. A novel L -glutamate oxidase from Streptomyces endus purification and properties [J]. Eur J Biochem, 1989, 182 (2): 327-332

[14] Fukunaga S, Yuno SH, Takahashi M, Taguchi S, Kera Y, Odani SH, Yamada RH. Puri fi cation and properties of D -glutamate oxidase from Candida boidinii 2201 [J]. J Ferment Bioeng, 1998, 85 (6): 579-583

[15] Oliveira MIP, Pimentel MC, Montenegro MCBSM, Araujo AN, Pimentel MF, Silva VL da. L-Glutamate determination in food samples by fl owinjection analysis [J]. Anal Chim Acta, 2001, 448 (1-2): 207-213

[16] Zhang M, Mullens C, Gorski W. Amperometric glutamate biosensor based on chitosan enzyme fi lm [J]. Electrochim Acta, 2006, 51: 4528-4532

[17] Cui Y, Barford JP, Renneberg R. Development of an L -glutamate biosensor using the coimmobilization of L -glutamate dehydrogenase and phydroxybenzoate hydroxylase on a Clark-type electrode [J]. Sens Actuators B, 2007, 127: 358-361

[18] Mankasingh U, Narinesingh D, Ngo TT. Quantitation of monosodium glutamate using immobilized glutamate oxidase/peroxidase and flow injection analysis [J]. Anal Lett, 2000, 33 (12): 2407-2423

[19] Muslim NZM, Ahmad M, Heng LY, Saad B. Optical biosensor test strip for the screening and direct determination of l-glutamate in food samples [J]. Sens Actuators B, 2012, 161: 493-497

[20] Ryth-Rinder. M, Kerekes N, Svensson M, H?kfelt T. Glutamate release from adult primary sensory neurons in culture is modulated by growth factors [J]. Regul Pept, 2001, 102: 69-79

[21] Wachiratianchi S, Bhumiratana A, Udomsopagit S. Isolation, purification and characterization of L -glutamate oxidase from Streptomyces sp. 18G [J]. Electron J Biotechnol, 2004, 7 (3): 275-276

[22] Volp e G, D r aisci R, Pal le sch i T, Compag none D. 3, 3 ′, 5, 5′-Tetramethylbenzidine as electrochemical substrate for horseradish peroxidase based enzyme immunoassays. A comparative study [J]. Analyst, 1998, 123: 1303-1307

[23] Goka AK, Farthing MJ. The use of 3, 3’, 5, 5’-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay[J]. J Immunol Assay, 1987, 8: 29-41

[24] Basu AK, Chattopadhyay P, Roychudhuri U, Chakrabor ty R. Development of biosensor based on immobilized L-glutamate oxidase for determination of monosodium glutamate in food [J]. Ind J Exp Biol, 2006 (44): 392-398

[25] European Parliament and Council Directives, No. 95/2/EC of 20 February 1995 on food additives other than colours and sweeteners [S]. Off. J. L061, 1995. 1-40