DOI: 10.3724/SP.J.1123.2011.00320

Chinese Journal of Chromatography (色谱) 2011/29:4 PP.320-324

A metabonomic study on the treatment of diabetic nephropathy with traditional Chinese medicine Tang-shen-fang

An ultra performance liquid chromatography-time of flight mass spectrometry (UPLC/TOF MS)-based method for plasma metabolic fingerprinting analysis was established. Acquired data were analyzed by principal component analysis and orthogonal projection to latent structure-discriminant analysis. The effect of Tang-shen-fang (TSF) on the treatment of diabetic nephropathy patients was evaluated. Significant changes were found after 3 and 6 months’ treatment of TSF compared with placebo controls. Nineteen metabolites in plasma were identified as potential biomarkers, including lipids, fatty acids and amino acids. The present metabonomic study is helpful to grasp the changes of global metabolic networks during the treatment of TSF and to testify its clinical efficacy and understand its action mechanism.

Key words:ultra performance liquid chromatography-time of flight mass spectrometry (UPLC/TOF MS),metabonomics,Tang-shen-fang decoction,diabetic nephropathy

ReleaseDate:2014-07-21 15:45:25

[1] Parving H H, Persson F, Lewis J B. N Engl J Med, 2008, 358(23): 2433

[2] Mitch W E. N Engl J Med, 2004, 351(19): 1934

[3] Lenz E M, Wilson I D. J Proteome Res, 2007, 6(2): 443

[4] Nicholson J K, Lindon J C, Holmes E, et al. Xenobiotica, 1999, 29(11): 1181

[5] Nicholson J K, Bollard M E, Lindon J C, et al. Nat Rev Drug Discov, 2002, 1(2): 153

[6] Nordstrom A, Lewensohn R. J Neuroimmune Pharmacol, 2010, 5: 4

[7] Qi X C, Zhang H Y, Liang Q L, et al. Chinese Traditional Patent Medicine (齐小城, 章弘扬, 梁琼麟, 等. 中成药), 2009, 31(1): 106

[8] Wu Z M, Huang Z Q, Lehmann R, et al. Chromatographia, 2009, 69(Suppl): S23

[9] Zhang H J, Li P, Zhao J B, et al. Journal of Beijing University of Traditional Chinese Medicine (张浩军, 李平, 赵静波, 等. 北京中医药大学学报), 2009, 32(4): 244

[10] Perkins B A, Aiello L P, Krolewski A S, et al. N Engl J Med, 2009, 361(1): 83

[11] Herrmann M L, Schleyerbach R, Kirschbaum B J. Immunopharmacology, 2000, 47(2/3): 273

[12] Sung S C, Kim K, Lee K A, et al. J Vasc Res, 2009, 46(1): 36

[13] Girao H, Catarino S, Pereira P. Cell Commun Sign, 2004, 2: 1

[14] Larrayoz I M, Huang J D, Lee J W, et al. Invest Ophth Vis Sci, 2010, 51(10): 4942

[15] Laczy B, Hill B G, Wang K, et al. Am J Physiol Heart Circ Physiol, 2009, 296: H13

[16] Marshall S, Bacote V, Traxinger R R. Biol Chem, 1991, 266: 4706

[17] Akimoto Y, Hart G W, Wells L, et al. Glycobiology, 2007, 17: 127

[18] Zsila F, Mady G. Biochem Biophys Res Commun, 2008, 372(3): 503

[19] Kim Y M, Pae H O, Park J E, et al. Antioxid Redox Sign, 2011, 14(1): 137