doi:

DOI: 10.3724/SP.J.1123.2013.01003

Chinese Journal of Chromatography (色谱) 2013/31:6 PP.510-513

Detection of the major milk allergenα-casein in food with liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode


Abstract:
A new method of detecting milk allergen protein in food was established. Using multiple reaction monitoring (MRM) mode in high performance liquid chromatography-mass spectrometry (HPLC-MS) to detect milk allergen protein α-casein is successful and convenient. All transitions gave a linear response in the chosen concentration range of the milk allergen protein (between 0.5 and 250 mg/L total soluble protein, 5 data points), and the detection limit is 0.5 mg/L of α-casein. Food samples from markets were tested. The results showed that MRM method could be used to detect the milk allergen in food products.

Key words:multiple reaction monitoring (MRM),liquid chromatography-mass spectrometry(LC-MS),milk allergen,α-casein,food

ReleaseDate:2015-04-16 10:33:11



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