DOI: 10.3724/SP.J.1123.2013.01003

Chinese Journal of Chromatography (色谱) 2013/31:6 PP.510-513

Detection of the major milk allergenα-casein in food with liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode

A new method of detecting milk allergen protein in food was established. Using multiple reaction monitoring (MRM) mode in high performance liquid chromatography-mass spectrometry (HPLC-MS) to detect milk allergen protein α-casein is successful and convenient. All transitions gave a linear response in the chosen concentration range of the milk allergen protein (between 0.5 and 250 mg/L total soluble protein, 5 data points), and the detection limit is 0.5 mg/L of α-casein. Food samples from markets were tested. The results showed that MRM method could be used to detect the milk allergen in food products.

Key words:multiple reaction monitoring (MRM),liquid chromatography-mass spectrometry(LC-MS),milk allergen,α-casein,food

ReleaseDate:2015-04-16 10:33:11

[1] Picariello G, Mamone G, Addeo F, et al. J Chromatogr A, 2011, 1218: 7386

[2] Stevenson S E, Houston N L, Thelen J J. Regulatory Toxicology and Pharmacology, 2010, 58: S36

[3] Guo L, Huo G C, Zhang Y H. Food Science and Technology (郭鸰, 霍贵成, 张英华. 食品科技), 2007(4): 262

[4] Zhang X, Wu D X, Liu G H, et al. Journal of Food Safety and Quality (张霞, 吴冬雪, 刘国红, 等. 食品安全质量检测学报), 2012, 3(4): 290

[5] Zhang Z J, Mao L T, Xiang J J, et al. Food Science and Technology (张在军, 毛露甜, 向军俭, 等. 食品科技), 2005(4): 69

[6] Lange V, Picotti P, Domon B, et al. Molecular Systems Biology, 2008, 4: 222. DOI: 10.1038/msb.2008.61

[7] Picotti P, Rinner O, Stallmach R, et al. Nature Methods, 2010, 7: 43

[8] Zhao Y, Jia W, Wang J F, et al. Anal Chem, 2011, 83: 8802

[9] Whiteaker J R, Zhang H, Zhao L, et al. J Proteome Res, 2007, 6: 3962

[10] Monaci L, Losito I, Palmisano F, et al. J Chromatogr A, 2010, 1217: 4300

[11] van Hengel A J. Anal Bioanal Chem, 2007, 389: 111

[12] Monaci L, Tregoat V, van Hengel A J, et al. Eur Food Res Technol, 2006, 223: 149

[13] Mao L T, Xiang J J, Zhang Z J. Food Science (毛露甜, 向军俭, 张在军. 食品科学), 2007, 28(5): 305

[14] Heick J, Fischer M, Popping B. J Chromatogr A, 2011, 1218: 938

[15] Ansari P, Stoppacher N, Rudolf J, et al. Anal Bioanal Chem, 2011, 399: 1105