DOI: 10.3724/SP.J.1123.2016.10001

Chinese Journal of Chromatography (色谱) 2017/35:3 PP.252-254

Specific recognition and detection of target proteins by new fluorescence molecularly imprinted membrane

A new method for producing L-cysteine-capped ZnS quantum dots (QDs) embedded molecularly imprinted membrane (QDs@MIM) was developed. The QDs@MIM was used as fluorescence artificial receptor for specific recognition and detection of target proteins. The QD@MIM was fabricated on a silylanized glass plate. Lysozyme, acrylamide, L-cysteine modified Mn2+-doped ZnS QDs and N,N'-methylenediacrylamide were used as template, functional monomer, assistant monomer and cross-linker, respectively. Under optimal conditions, the linear range for lysozyme ranged from 0.1 to 1.0 μmol/L, adsorption time was 4 min and the imprinting factor for lysozyme was 6.2. The results showed that the molecularly imprinted polymer can be used as a simple, rapid and selective biosensor to detect target proteins in biologic samples.

Key words:molecularly imprinted membranes (MIM),quantum dots (QDs),protein detection

ReleaseDate:2017-03-21 16:31:24

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