DOI: 10.3724/SP.J.1123.2016.10021

Chinese Journal of Chromatography (色谱) 2017/35:3 PP.255-259

Synthesis of functional mesoporous silica and selective adsorption of surface-exposed histidine proteins

The Cu2+immobilized mesoporous silica particles (PPOSS-IDA-Cu2+) were fabricated. The prepared materials were applied to the highly specific separation of surface-exposed histidine proteins, based on immobilized metal ion affinity chromatography. The adsorption experiments of bovine serum albumin, myoglobin, lysozyme and bovine hemoglobin (BHb) exhibited that PPOSS-IDA-Cu2+ had excellent performance in the separation of protein BHb. The maximum binding capacity of the proposed materials for BHb was 3150 mg/g. The results also demonstrated that PPOSS-IDA-Cu2+ had potential application in removing highly abundant proteins in proteomic analysis, and can be combined with other techniques to get more information on low abundant proteins.

Key words:free-radical polymerization,mesoporous silica particles,polymeric polyhedral oligomeric silsesquioxane-iminodiacetic acid-Cu2+ (PPOSS-IDA-Cu2+),surface-exposed histidine,bovine hemoglobin (BHb)

ReleaseDate:2017-03-21 16:31:24

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