doi:

DOI: 10.3724/SP.J.1123.2016.10010

Chinese Journal of Chromatography (色谱) 2017/35:3 PP.264-268

Capillary ion chromatography-mass spectrometry for determination of low relative molecular mass organic acids in culture media of Clostridium thermocellum


Abstract:
A capillary ion chromatography-mass spectrometry method was proposed to determine low relative molecular mass organic acids in culture media of Clostridium thermocellum. The conditions of analysis were optimized and eight organic acids were detected simultaneously. The target compounds were separated on an IonSwift MAX-100 capillary column with potassium hydroxide solution as mobile phase by gradient elution, and detected under selected ion monitoring (SIM) mode. The mass spectrometry conditions were: spray voltage 3.0 kV, sheath gas 2000 kPa, capillary temperature 275℃. The relative standard deviations ranged from 1.45% to 5.99%. The coefficients of determination ranged from 0.9696 to 0.9986 and the mean recoveries were 89.0%-110.0%. The limits of detection of the eight organic acids were 0.01-0.50mg/L. This method was used to detect low relative molecular mass organic acids in culture media of Clostridium thermocellum with high sensitivity and good reproducibility.

Key words:capillary ion chromatography,mass spectrometry (MS),organic acids,Clostridium thermocellum

ReleaseDate:2017-03-21 16:31:25



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