doi:

DOI: 10.3724/SP.J.1123.2016.10022

Chinese Journal of Chromatography (色谱) 2017/35:3 PP.302-307

Separation and purification of flavonoids from Mikania micrantha by macroporous resin combined with high speed countercurrent chromatography


Abstract:
The present study established a method for the separation and purification of four flavonoids quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin from Mikania micrantha by using macroporous resin combined with high speed countercurrent chromatography (HSCCC). Macroporous resin was AB-8 and the eluent was 50% (v/v) ethanol. A two-phase solvent system composed of n-butanol/acetic acid/water (4:1:5, v/v) was used in HSCCC. Quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin with the purity of 90.12%, 98.55%, 98.33% and 99.23%, respectively, were successfully separated from Mikania micrantha by HSCCC using the lower phase of this solvent system as mobile phase in one run. This study pave a simple and efficient method that could be used in the following separation of flavonoids from invasive plants.

Key words:high speed countercurrent chromatography (HSCCC),macroporous resin,separation,flavonoids,Mikania micrantha (M. micrantha)

ReleaseDate:2017-03-21 16:31:26



[1] Ma L, Qiang S. Weed Sci, 2006(1): 55

[2] Kong G H, Wu Q G, Hu Q M. Journal of Tropical and Subtropical Botany, 2000, 8(2): 128

[3] Feng H L, Cao H L, Liang X D. Journal of Tropical and Subtropical Botany, 2002, 10(3): 263

[4] Zhang L Y, Ye W H, Cao H L, et al. Weed Res, 2004, 44(1): 42

[5] Rufatto L C, Gower A, Schwambach J, et al. Rev Bras Farmacogn, 2012, 22: 1384

[6] Aguinaldo A M, Padolina W G, Abe F, et al. Biochem Syst Ecol, 2003, 31(6): 665

[7] Wei X, Huang H, Wu P, et al. Biochem Syst Ecol, 2004, 32(12): 1091

[8] Eunice R V, Leon A, Chavez M I, et al. Fitoterapia, 2014, 94: 155

[9] Havsteen B H. Pharmacol Therapeut, 2002, 96(2/3): 67

[10] Testai L. Life Sci, 2015, 135: 68

[11] Ito Y. J Chromatogr A, 2005, 1065: 145

[12] Hu R L, Pan Y J. TrAC-Trends Anal Chem, 2012, 40: 15

[13] Li Z Q, Li Q Y, Jiang X L, et al. Chinese Journal of Chromatography, 2014, 32(12): 1404

[14] Xie Q Q, Wei Y, Zhang G L. Sep Purif Technol, 2010, 72(2): 229

[15] Sun Y J, Sun Y S, Chen H, et al. J Chromatogr B, 2014, 969: 190

[16] Liu Y L, Chen T, Chen C, et al. Chinese Journal of Chromatography, 2014, 32(5): 543

[17] Wei Y, Huang W W, Gu Y X. J Chromatogr A, 2013, 1284: 53

[18] Wang Y Q, Wu D, Zhao X Z, et al. Chinese Journal of Chromatography, 2016, 34(8): 788

[19] Huang Y Y, Liu X F, Liu J Z, et al. J Taiwan Inst Chem E, 2016, 67: 61

[20] Li L, Liu J Z, Luo M, et al. J Chromatogr B, 2016, 1033/1034: 40

[21] Dhanarajan G, Rangarajan V, Sen R. Sep Purif Technol, 2015, 143(25): 72

[22] Wang R, Peng X G, Wang L M, et al. J Sep Sci, 2012, 35(15): 1985

[23] Shaheen N, Yin L, Gu Y X, et al. J Sep Sci, 2015, 38(11): 1933

[24] Guo M, Liang J, Wu S. J Chromatogr A, 2010, 1217: 5398

[25] Qu L P, Xin H L, Su Y H, et al. J Sep Sci, 2012, 35(7): 883

[26] Zhang J K, Zhu X Y, Luo F L, et al. J Sep Sci, 2012, 35(1): 128