doi:

DOI: 10.3724/SP.J.1123.2017.03007

Chinese Journal of Chromatography (色谱) 2017/35:6 PP.594-600

Determination of 34 illegally adulterated weight loss compounds in foods by ultra high performance liquid chromatography- triple quadrupole mass spectrometry


Abstract:
An analytical method was developed for the determination of 34 illegally adulterated weight loss compounds in foods and dietary supplements by ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS). A Waters CORTECS T3 column (100 mm×2.1 mm, 2.7 μm) was used with 0.1% (v/v) formic acid aqueous solution-acetonitrile containing 0.1% (v/v) formic acid as mobile phases by gradient elution. The compounds were detected by electrospray ion source in positive or negative mode with multiple reaction monitoring (MRM) mode. The calibration curves showed good linearity in the range of 0.5-10 μg/L for 29 compounds such as sibutramine, and in the range of 2.5-50 μg/L for five compounds such as chlorothiazide. The correlation coefficients (r) of the standard calibration curves for the 34 analytes were all greater than 0.99. The recoveries of the 29 compounds at spiked levels of 5, 10 and 20 μg/kg were in the range of 49.2%-136.2%, and the RSDs were 0.7%-15.0% (n=6). The recoveries of the five compounds at spiked levels of 25, 50 and 100 μg/kg were in the range of 51.5%-130.9%, and the RSDs were 0.8%-14.0% (n=6). The limits of detection (LODs) and limits of quantification (LOQs) were 5 μg/kg and 10 μg/kg for the 29 compounds, 25 μg/kg and 50 μg/kg for the five compounds, respectively. The method was successfully applied to the analysis of actual samples, and 12 compounds were checked out, which combated the illegal adulteration behavior effectively.

Key words:ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS),illegal adulteration,weight loss compounds,foods

ReleaseDate:2017-06-16 16:24:25



[1] Food and Drug Administration. Beware of Products Promising Miracle Weight Loss. (2015-07-25) [2017-04-05]. https: //www.fda.gov/forconsumers/consumerupdates/ucm246742. htm

[2] Wozniak K S, Georgiev M I, Orhan I E. Food Chem Toxicol, 2016, 6: 1

[3] Huang N J, Yang W H, Huang Y B. Food and Drug, 2011, 13(3): 114 黄诺嘉, 杨文红, 黄奕滨. 食品与药品, 2011, 13(3): 114

[4] Wan L C, Wang D, Ding Y P, et al. Chinese Pharmaceutical Affairs, 2013, 27(12): 1285 万林春, 王栋, 丁银平, 等. 中国药事, 2013, 27(12): 1285

[5] Wang J W, Huang X L, Cao J, et al. Chinese Journal of Chromatography, 2014, 32(2): 151 王静文, 黄湘鹭, 曹进, 等. 色谱, 2014, 32(2): 151

[6] Zeng Y, Xu Y M, Kee C L, et al. Drug Test Anal, 2016, 8: 351

[7] Wang K, Hu Q, Cui Y L, et al. Chinese Journal of Pharmaceutical Analysis, 2008, 28(8): 1268 王柯, 胡青, 崔益泠, 等. 药物分析杂志, 2008, 28(8): 1268

[8] Wang J, Chen B, Yao S. Food Addit Contam A, 2008, 25(7): 822

[9] Rebiere H, Guinot P, Civade C, et al. Food Addit Contam A, 2012, 29(2): 161

[10] Kim H J, Lee J H, Park H J, et al. Food Addit Contam A, 2014, 31(5): 777

[11] Liang Q L, Qu J, Luo G A, et al. J Pharm Biomed Anal, 2006, 40: 305

[12] Yu H, Hu Q, Ji S, et al. Journal of Food Safety & Quality, 2016, 7(7): 2704 于泓, 胡青, 季申, 等. 食品安全质量检测学报, 2016, 7(7): 2704

[13] Vaysse J, Balayssac S, Gilard V, et al. Food Addit Contam A, 2010, 27(7): 903

[14] Liu B, Yuan L P, Nie Y H, et al. Modern Food Science and Technology, 2016, 32(8): 290 刘波, 袁利鹏, 聂燕华, 等. 现代食品科技, 2016, 32(8): 290

[15] Lu H, Qian Y F, Zhang B, et al. Science and Technology of Food Industry, 2016, 37(10): 88 鲁辉, 钱叶飞, 张斌, 等. 食品工业科技, 2016, 37(10): 88

[16] Ma W, Peng T, Zhu M D, et al. Chinese Journal of Analytical Chemistry, 2009, 37(11): 1583 马微, 彭涛, 朱明达, 等. 分析化学, 2009, 37(11): 1583

[17] Gong X, Lu L, Feng Y L, et al. Chinese Journal of Pharmaceutical Analysis, 2016, 36(5): 918 宫旭, 芦丽, 冯有龙, 等. 药物分析杂志, 2016, 36(5): 918