doi:

DOI: 10.3724/SP.J.1123.2016.12028

Chinese Journal of Chromatography (色谱) 2017/35:6 PP.601-607

Simultaneous determination of five mycotoxins in various grains and their products by liquid chromatography-tandem mass spectrometry with isotope internal standard


Abstract:
A rapid method was established for the determination of zearalenone (ZON), nivalenol (NIV), deoxynivalenol (DON), 3-acetyl-deoxynivalenol (3-ACDON) and 15-acetyl-deoxynivalenol (15-ACDON) in various grains and their products by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted by acetonitrile-water (84:16, v/v), and cleaned-up by multifunctional column without any further step. The separation was carried out on an Acquity UPLC® BEH C18 column (100 mm×2.1 mm, 1.7 μm) with gradient elution using acetonitrile-water as mobile phases. The analytes were quantified by isotope internal standard method. The correlation coefficients (r2) were all above 0.99. The limits of detection (LODs, S/N=3) ranged from 5 μg/kg to 20 μg/kg. The recoveries of the five mycotoxins spiked in blank grain and its product samples varied from 84.2% to 114.5%, with the relative standard deviations (RSDs) of 0.4%-9.9% (n=6). The method is accurate, efficient, sensitive and practical.

Key words:liquid chromatography-tandem mass spectrometry (LC-MS/MS),isotope internal standard,mycotoxins,grains and their products

ReleaseDate:2017-06-16 16:24:25



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