doi:

DOI: 10.3724/SP.J.1123.2017.02010

Chinese Journal of Chromatography (色谱) 2017/35:6 PP.627-633

Simultaneous determination of 30 mycotoxins in different feed products by ultra high performance liquid chromatography-tandem mass spectrometry


Abstract:
A method for the simultaneous determination of 30 mycotoxins in different feed products was developed using a QuEChERS pretreatment procedure coupled with ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The samples were extracted with 5 mL water and 5 mL acetonitrile containing 1% (v/v) formic acid. An aliquot of the supernatant was dried by nitrogen gas, re-dissolved by 1mL water containing 5 mmol/L ammonium acetate-acetonitrile (80:20, v/v), and analyzed by UHPLC-MS/MS. Matrix-matched calibration curves and internal standards were used for accurate quantification. Satisfactory recoveries at low, medium and high spiked levels were ranged from 72.0% to 118.4% (n=5). Good linear relationships were obtained, the correlation coefficients (r2) were greater than 0.99. The limits of detection (LODs, S/N=3) and the limits of quantification (LOQs, S/N=10) ranged from 0.7 μg/L to 20 μg/L and from 2 μg/L to 50 μg/L, respectively. The proposed method is simple, rapid and valuable, which can be a powerful tool for quantitative analysis of the 30 mycotoxins in premixed feed, concentrated feed and formula feed products.

Key words:ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS),QuEChERS,mycotoxins,feed

ReleaseDate:2017-06-16 16:24:26



[1] Cao X Q, Wu S C, Yue Y, et al. J Chromatogr B, 2013, 942/943: 113

[2] Li F Q, Yu C C, Shao B, et al. Chinese Journal of Preventive Medicine, 2011, 45(1): 57 李凤琴, 于钏钏, 邵兵, 等. 中华预防医学杂志, 2011, 45(1): 57

[3] Binder E M, Tan L M, Chin L J, et al. Anim Feed Sci Technol, 2007, 137: 265

[4] Li P, Lai W H, Jin J. Academic Periodical of Farm Products Processing, 2005(3): 12 李鹏, 赖卫华, 金晶. 农产品加工学刊, 2005(3): 12

[5] World Health Organization (WHO). WHO Global Strategy for Food Safety: Safer Food for Better Health. Switzerland: WHO, 2002

[6] GB 2761-2011

[7] GB 13078. 2-2006

[8] GB 13078. 3-2007

[9] Li Y S, Luo X S, Yang S P, et al. J Agric Food Chem, 2014, 62: 1492

[10] Shephard G S. Chem Soc Rev, 2008, 37: 2468

[11] Wang J S, Zhou Y, Wang Q M. Food Chem, 2008, 107: 970

[12] Almeida I, Martins H M, Santos S, et al. Mycotox Res, 2011, 27: 177

[13] Monbaliu S, van Poucke C, Detavernier C, et al. J Agric Food Chem, 2010, 58(1): 66

[14] Zhao Z Y, Rao Q X, Song S Q, et al. J Chromatogr B, 2014, 963: 75

[15] Ying Y F, Zhu C Y, Wei M J, et al. Chinese Journal of Analytical Chemistry, 2010, 38(12): 1759 应永飞, 朱聪英, 韦敏珏, 等. 分析化学, 2010, 38(12): 1759

[16] Dong M F, Si W S, Jiang K Q, et al. J Chromatogr A, 2015, 1423: 177

[17] Anastassiades M, Lehotay S J, Stajnbaher D, et al. J AOAC Int, 2003, 86(2): 412

[18] Gong X M, Ma R H, Wang H T, et al. Chinese Journal of Chromatography, 2017, 35(2): 185 宫小明, 马荣桧, 王洪涛, 等. 色谱, 2017, 35(2): 185

[19] He J L, Peng T, Xie J, et al. Chinese Journal of Chromatography, 2016, 34(7): 708 何建丽, 彭涛, 谢洁, 等. 色谱, 2016, 34(7): 708