doi:

DOI: 10.3724/SP.J.1123.2016.12041

Chinese Journal of Chromatography (色谱) 2017/35:6 PP.643-649

A novel pattern for predicting the quality of Chinese herba preparation intelligently by high performance liquid chromatographic formula fingerprints


Abstract:
In order to build the fusion models, the high performance liquid chromatographic (HPLC) fingerprints of scutellariae radix (SR), rhei radix et rhizoma (RRR), coptidis rhizoma (CR) and their synthesizing fingerprints were developed in this study. After exploring the consistency between the fingerprints of compound synthesizing fingerprints (CSF) and the sample, the quality of traditional Chinese medicine preparation was predicted intelligently using CSF. HPLC coupled with diode array detector was used to obtain chromatograms of SR, RRR, CR and Yi Qing Tablet (YQT) samples at 268 nm. Meanwhile, the quality of CSF and the 15 batches of YQT samples was evaluated by systematically quantified fingerprint method (SQFM) qualitatively and quantitatively. The chromatograms showed that CSF covered the main fingerprints' information of each herb and the 55 common peaks of CSF covered the main information of the 50 common peaks in YQT sample. The evaluation results showed that among the 15 batches of YQT samples, only YQT-S01 was grade 5 and the others were all above grade 3. Most of the CSFs were grade 2 or grade 1 except CSF-2 which was grade 6. The fingerprints of Chinese herba preparation could be replaced by CSF to achieve a novel pattern for predicting the quality of TCM preparation intelligently by studying the relationship between the standard fingerprints and the CSF, and simultaneously developing first-class evaluation software.

Key words:high performance liquid chromatographic (HPLC),compound synthesizing fingerprints (CSF),intelligent prediction,quality control of traditional Chinese medicine (TCM)

ReleaseDate:2017-06-16 16:24:27



[1] Pharmacopoeia Commission of the People's Republic of China. Pharmacopoeia of the People's Republic of China, Part 1. Beijing: China Medical Science Press, 2010: 22 国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社, 2010: 22

[2] Yang Y, Wang H J, Yang J, et al. J Chromatogr A, 2013, 1321: 88

[3] Han J, Ye M, Xu M, et al. J Chromatogr B, 2007, 848: 355

[4] Luo R, Wang J B, Zhao L, et al. Bioorg Med Chem Lett, 2014, 24: 1334

[5] Deng Y X, Zhang X J, Shi Q Z, et al. J Ethnopharmacology, 2012, 144: 425

[6] Zhang H F, Shi Y P. Talanta, 2010, 82: 1010

[7] Gong L L, Yin X J, Wang L, et al. Chinese Journal of Pharmacovigilance, 2015, 12(1): 1 贡磊磊, 殷小杰, 王岚, 等. 中国药物警戒, 2015, 12(1): 1

[8] Feng Y L, Yu B Y, Wang Y. Chinese Journal of Hospital Pharmacy, 2008, 28(17): 1521 冯有龙, 余伯阳, 王玉. 中国医院药学杂志, 2008, 28(17): 1521

[9] Bao X, Bao D Y, Hu K H, et al. Sichuan Journal of Physiological Sciences, 2001, 23(3): 130 包旭, 包定元, 胡开华. 四川生理科学杂志, 2001, 23(3): 130

[10] Luo G L, Zheng X Y. Journal of Pharmaceutical Research, 2013, 32(2): 112 罗格莲, 郑孝勇. 药学研究, 2013, 32(2): 112

[11] Sun G X, Shi X F, Zhang J X, et al. Acta Pharmaceutica Sinica, 2008, 43(10): 1047 孙国祥, 史香芬, 张静娴, 等. 药学学报, 2008, 43(10): 1047

[12] Yang F L, Zhang J, Sun G X, et al. Chinese Journal of Chromatography, 2016, 34(7): 715 杨方良, 张晶, 孙国祥, 等. 色谱, 2016, 34(7): 715

[13] Sun G X, Yan B, Hou Z F, et al. Central South Pharmacy, 2015, 13(7): 673 孙国祥, 闫波, 侯志飞, 等. 中南药学, 2015, 13(7): 673

[14] Liu W, Jiang S Y. Chinese Pharmacy, 2011, 22(19): 1819 刘文, 蒋世云. 中国药房, 2011, 22(19): 1819

[15] Chen H Y. Chinese Journal of Information on Traditional Chinese Medicine, 2012, 19(5): 109 陈红英. 中国中医药信息杂志, 2012, 19(5): 109

[16] Qin K M, Fang Q B, Cai H, et al. Chinese Journal of Chromatography, 2009, 27(6): 781 秦昆明, 方前波, 蔡皓, 等. 色谱, 2009, 27(6): 781

[17] Qi J, Yu B Y. Chinese Journal of Natural Medicines, 2010, 8(3): 171 戚进, 余伯阳. 中国天然药物, 2010, 8(3): 171

[18] Zhao B N, Yu Z Y, Ding X Y, et al. Chinese Traditional and Herbal Drugs, 2011, 42(2): 380 赵渤年, 于宗渊, 丁晓彦, 等. 中草药, 2011, 42(2): 380

[19] Sun G X, Hu Y S, Bi K S. Acta Pharmaceutica Sinica, 2009, 44(4): 401 孙国祥, 胡玥珊, 毕开顺. 药学学报, 2009, 44(4): 401

[20] Sun G X, Wu Y, Meng L X, et al. Central South Pharmacy, 2014, 12(3): 193 孙国祥, 吴玉, 孟令新, 等. 中南药学, 2014, 12(3): 193

[21] Pharmacopoeia Commission of the People's Republic of China. Pharmacopoeia of the People's Republic of China, Part 1. Beijing: China Medical Science Press, 2015: 374 国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社, 2015: 374