DOI: 10.3724/SP.J.1123.2017.06016

Chinese Journal of Chromatography (色谱) 2017/35:10 PP.1100-1104

Determination of guanidinoacetic acid in feed by ion chromatography coupled with ultraviolet detection

A novel ion chromatography method for the determination of guanidinoacetic acid (GAA) in animal feed samples was developed. The optimal detection mode, chromatography column, gradient condition of mobile phases and sample pretreatment conditions were investigated. Using a linear gradient of methane sulfonic acid and ultraviolet detector at 200 nm, the interfering substances in feed samples and GAA could be separated successfully on a Dionex IonPacTM CS16 cation exchange analysis column. The calibration curves showed a range of linearity between 0.5 and 200 mg/L, and a high linear regression coefficient of 0.9999 was obtained for GAA. The limits of detection and limits of quantification of GAA in formula feed and concentrate feed were 4.5 and 15 mg/kg, respectively. The limit of detection and limit of quantification of GAA in compound-premix were 9.0 and 30 mg/kg, respectively. The recoveries of this method for GAA were investigated, and the recoveries were all more than 94% in formula feed, concentrate feed and compound-premix feed for pig and poultry. This method can meet the requirements of the determination of GAA in feed samples for the feed production enterprise and evaluation center.

Key words:ion chromatography (IC),ultraviolet (UV) detection,cation exchanged,guanidinoacetic acid (GAA),feed

ReleaseDate:2017-11-03 11:22:12

[1] Zhang D F, Li J T, Tian Y Y, et al. Chinese Journal of Animal Nutrition, 2017, 29(2):456 张德福, 李军涛, 田耀耀, 等. 动物营养学报, 2017, 29(2):456

[2] Pan B H, Sun D Y, Tian Y Y. Chinese Journal of Animal Science, 2016, 52(19):38 潘宝海, 孙冬岩, 田耀耀. 中国畜牧杂志, 2016, 52(19):38

[3] Liu Y, Li J L, Li Y J, et al. Anim Feed Sci Tech, 2015, 205:82

[4] Ministry of Agriculture. No. 2167 Bulletin of the Ministry of Agriculture of the People's Republic of China. (2014-10-23)[2017-08-02]. 农业部. 中华人民共和国农业部公告第2167号. (2014-10-23)[2017-08-02].

[5] European Union. No. 904/2009 Commission Regulation of European Union. (2009-09-28)[2017-08-02]. 欧盟. 欧盟委员会条例第904/2009号. (2009-09-28)[2017- 08-02].

[6] European Union. No. 2016/1768 Commission Implementing Regulation of European Union. (2016-10-04)[2017-08-02]. 欧盟. 欧盟委员会执行条例第2016/1768号. (2016-10-04)[2017-08-02].

[7] Li X Y, Han K, Han Y N, et al. Chinese Journal of Analysis Laboratory, 2015, 34(8):886 李秀瑜, 韩康, 韩亚楠, 等. 分析试验室, 2015, 34(8):886

[8] Carduccia C, Birarellia M, Santagataa P. J Chromatogr B, 2001, 755:343

[9] Buchberger W, Ferdig M. J Sep Sci. 2004, 27:1309

[10] Kandhro A J, Khuhawar M Y. J Liq Chromatogr R T, 2010, 33:1393

[11] Song W D, Yuan X Y, Lü N, et al. Chinese Journal of Chromatography, 2016, 34(11):1084 宋卫得, 袁晓鹰, 吕宁, 等. 色谱, 2016, 34(11):1084

[12] Liu Y Q, Yu H, Journal of Instrumental Analysis, 2015, 34(6):734 刘永强, 于泓. 分析测试学报, 2015, 34(6):734

[13] Zhang S F, Sheng H D, Jiang K, et al. Chinese Journal of Chromatography, 2016, 34(10):946 张水锋, 盛华栋, 姜侃, 等. 色谱, 2016, 34(10):946

[14] Le S F, Wang W, Gao X, et al. Chinese Journal of Food Safety & Quality, 2016, 7(11):4333 乐胜锋, 王尉, 高峡, 等. 食品安全质量检测学报, 2016, 7(11):4333

[15] Song W D, Su Z, Hui X D, et al. Chinese Journal of Food Research and Development, 2016, 37(15):217 宋卫得, 苏征, 惠希东, 等. 食品研究与开发, 2016, 37(15):217

[16] Li W L, Wu H T, Fan X P, et al. Chinese Journal of Veterinary Medicine, 2016, 52(8):110 李伟岭, 吴好庭, 范小平, 等. 中国兽医杂志, 2016, 52(8):110