DOI: 10.3724/SP.J.1123.2017.09021

Chinese Journal of Chromatography (色谱) 2018/36:1 PP.37-42

Separation of chemical compositions in root of Rumex patientia L. with off-line two-dimensional liquid chromatography

An innovative analytical method based on the off-line two-dimensional reversed-phase/reversed-phase liquid chromatography (2D-RPLC/RPLC) was established to separate the components of root of Rumex patientia L. The chromatograms of ethyl acetate extract of root of Rumex patientia L. on a phenyl/tetrazolium column (250 mm×4.6 mm, 7 μm) and a Unitary C18 column (250 mm×4.6 mm, 7 μm) were compared, and they showed different separation abilities. A phenyl/tetrazolium column was used for the first dimensional separation with 0.1% (v/v) formic acid aqueous solution and methanol as mobile phases, and 18 fractions was collected. The second dimensional liquid chromatography analysis was carried out on a Unitary C18 column with 0.1% (v/v) formic acid aqueous solution and methanol as mobile phases. Based on the experiment setup and results, it was concluded that off-line 2D-LC can be an effective method for the separation of the trace components and the screening of active compounds of root of Rumex patientia L.

Key words:two-dimensional liquid chromatography (2D-LC),phenyl/tetrazolium bonded stationary phase,anthraquinone,Rumex patientia L.

ReleaseDate:2018-01-24 14:42:46

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