doi:

DOI: 10.3724/SP.J.1123.2017.10026

Chinese Journal of Chromatography (色谱) 2018/36:2 PP.85-93

Preparation of hydrophilic polymer brushes magnetic microspheres by surface-initiated for magnetic dispersive solid phase extraction of tetracycline antibiotic residues from honey


Abstract:
Activators regenerated by electron transfer-atom transfer radical polymerization (ARGET-ATRP) was applied to the continuous grafting of polybasic polymers and poly(ethylene glycol) (PEG) brushes on the surface of the magnetic microspheres (MMs). At first, the MMs were coated with silica gel, modified by amino group, and then 2-bromoisobutyryl bromide was grafted on the surface of MMs. After that, the hydrophilic polymer brushes magnetic microspheres (HMMs) were prepared by polymerization on the surface of the MMs. HMMs were characterized by transmission electron microscope (TEM) and Fourier transform-infrared (FT-IR) spectrometer. The adsorption performance to protein was studied. The results demonstrated that HMMs have the relatively uniform particle size, good dispersity and excellent protein resistance properties. Tetracycline antibiotics (TCs, tetracycline hydrochloride, chlortetracycline hydrochloride and doxycycline hydrochloride) in honey samples were determined by magnetic dispersion solid phase extraction (MDSPE) using the prepared HMMs and high performance liquid chromatography (HPLC). The average recoveries were obtained in the range of 85.8%-94.5%. The limits of detection (LODs) and limits of quantification (LOQs) of the proposed method were in the ranges of 1.92-2.56 μg/kg and 6.40-8.53 μg/kg, respectively. The proposed method was successfully applied to fast clean-up and enrichment of the TCs residues in honey.

Key words:magnetic dispersive solid phase extraction (MDSPE),activators regenerated by electron transfer-atom transfer radical polymerization (ARGET-ATRP),hydrophilic polymer brushes,magnetic microspheres (MMs),tetracycline antibiotics (TCs),honey

ReleaseDate:2018-03-21 07:43:00



[1] Code of Federal Regulations (CFR), Title 21, Part 556. Tolerances for Residues of New Animal Drugs in Food. (2013-04-01)[2017-11-18]. https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?CFRPart=820&showFR=1

[2] 2377/90/EEC

[3] Ministry of Agriculture. No. 235 Bulletin of the Ministry of Agriculture of the People's Republic of China. (2002-12-24)[2017-11-18]. http://yz.hz-agri.gov.cn/uploadFiles/2005-10/1130221564406.doc农业部. 中华人民共和国农业部公告第235号. (2002-12-24)[2017-11-18]. http://yz.hz-agri.gov.cn/uploadFiles/2005-10/1130221564406.doc

[4] GB/T 22990-2008

[5] Phiroonsoontorn N, Sansuk S, Santaladchaiyakit Y, et al. J Chromatogr A, 2017, 1519:38

[6] Lv Y K, Wang L M, Yang L, et al. J Chromatogr A, 2012, 1227(5):48

[7] GB/T 23409-2009

[8] Xu H, Mi H Y, Guan M M, et al. Food Chem, 2017, 232:198

[9] Guo M, Yu F, Jia K L, et al. Chinese Journal of Chromatography, 2016, 34(4):407国明, 于峰, 贾科玲, 等. 色谱, 2016, 34(4):407

[10] Rios A, Zougagh M, Bouri M. Anal Methods, 2013, 5(18):4558

[11] Li X S, Zhu G T, Luo Y B, et al. TrAC-Trends Anal Chem, 2013, 45(4):233

[12] Lv Y K, Zhao C X, Li P, et al. J Sep Sci, 2013, 36(16):2656

[13] Lv Y K, He Y D, Xiong X, et al. New J Chem, 2015, 39(3):1792

[14] Lv Y K, Zhang J, Li M Z, et al. Anal Methods, 2016, 8(19):3982

[15] Lv Y K, Xiong X, Zhao F F, et al. Aust J Chem, 2017, 70(3):237

[16] Cao X T, Horak D, An Z S, et al. J Polym Sci Pol Chem, 2016, 54(8):1036

[17] Yang Y, Liu X, Ye G, et al. ACS Appl Mater Inter, 2017, 9(15):13637

[18] Xie L, Lan F, Li W, et al. Colloids Surf B Biointerfaces, 2014, 123:413

[19] Yang Y Q, Zhao B, Li Z D, et al. Acta Biomater, 2013, 9(8):7679

[20] Inoue Y, Nakanishi T, Ishihara K. React Funct Polym, 2011, 71(3):350