doi:

DOI: 10.3724/SP.J.1123.2017.09022

Chinese Journal of Chromatography (色谱) 2018/36:2 PP.94-99

Preparation and evaluation of a new type of amino-bonded silica chromatographic stationary phase for the determination of lactose


Abstract:
A method for the separation and determination of lactose and sucrose using high performance liquid chromatography-refractive index (HPLC-RI) detection base on the prepared amino-bonded silica gel chromatographic stationary phase was established. The method was accorded to the content determination method in Pharmacopoeia of the People's Republic of China (2015). Then, a series of chromatographic behaviors of lactose and sucrose such as the retention times, resolutions and peak area stabilities were investigated on three different types of amino-bonded silica columns (300 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-water (70:30, v/v) with isocratic elution, the flow rate was 1.0 mL/min and the injection volume was 10 μL. The resolution of lactose and sucrose was 3.03 by isopropyl side chain protected amino column, and the peak shape of each target compound was good. The relative standard deviation (RSD) of peak area of lactose was 1.14%, less than 2.0% prescribed in the Pharmacopoeia of the People's Republic of China (2015). Therefore, this method meets the requirements of lactose content determination, and is suitable as the quality control method in the above mentioned Pharmacopoeia.

Key words:amino-bonded silica gel chromatographic stationary phase,high performance liquid chromatography (HPLC),preparation,lactose,sucrose,Pharmacopoeia of the People's Republic of China (2015)

ReleaseDate:2018-03-21 07:43:00



[1] Yue H, Zhao Z, Liu L J, et al. Journal of Dairy Science and Technology, 2017, 40(2):23岳虹, 赵贞, 刘丽君, 等. 乳业科学与技术, 2017, 40(2):23

[2] Li Q, Zhang L W, Zhang Y C, et al. Food Science, 2012, 33(4):162李琦, 张兰威, 张英春, 等. 食品科学, 2012, 33(4):162

[3] Shang S, Feng Y L. Northwest Pharmaceutical Journal, 2014, 29(1):27尚姝, 冯有龙. 西北药学杂志, 2014, 29(1):27

[4] Luo L H, Gao X J, Wei R, et al. Chinese Journal of Biologicals, 2011, 24(8):964雒丽红, 高雪军, 魏然, 等. 中国生物制品学杂志, 2011, 24(8):964

[5] Ding H L, Li C, Jin P, et al. Chinese Journal of Chromatography, 2013, 31(8):804丁洪流, 李灿, 金萍, 等. 色谱, 2013, 31(8):804

[6] Wu J R. Chinese Journal of Tropical Agriculture, 2014, 33(6):55吴基任. 热带农业科学, 2014, 33(6):55

[7] Pan C, Zheng X Y, Huang H X, et al. Journal of Instrumental Analysis, 2015, 34(10):1107潘城, 郑小严, 黄红霞, 等. 分析测试学报, 2015, 34(10):1107

[8] Fu Q, Liang X M, Liang T, et al. China Patent, 201310090809.3. 2013-03-20傅青, 梁鑫淼, 梁图, 等. 中国专利, 201310090809.3. 2013-03-20

[9] Linden J C, Lawhead C L. J Chromatogr A, 1975, 105:125

[10] Kazlowski B, Pan C L, Tih Ko Y. Carbohydr Res, 2008, 343(14):2443

[11] GB/T 5009.8-2016

[12] Pharmacopoeia Commission of People's Republic of China. Pharmacopoeia of People's Republic of China, Part 1. Beijing:China Medical Science Press, 2015:524国家药典委员会. 中华人民共和国药典, 一部. 北京:中国医药科技出版社, 2015:524

[13] Shen G B, Zhang F F, Yang B C, et al. Talanta, 2013, 115(10):129

[14] Hemstrom P, Irgum K. J Sep Sci, 2006, 29(12):1784

[15] Wang Q J. China Patent, 200610015584.5. 2007-04-11汪群杰. 中国专利, 200610015584.5. 2007-04-11

[16] Snyder L R, Kirkland J J, Dolan J W. Introduction to Modern Liquid Chromatography. 3rd ed. Hoboken:John Wiley & SonsInc., 2010

[17] Li H T, Wei J F, Wang Q J. Chemical Reagents, 2016, 38(2):141李海涛, 魏俊富, 汪群杰. 化学试剂, 2016, 38(2):141

[18] Liu G Q, Yu Z L. Chromatographic Column Technology. 2nd ed. Beijing:Chemical Industry Press, 2005刘国荃, 余兆楼. 色谱柱技术. 2版. 北京:化学工业出版社, 2005

[19] Yu S L. High Performance Liquid Chromatography and Its Application. 2nd ed. Beijing:Chemical Industry Press, 2005于世林. 高效液相色谱方法及应用. 2版. 北京:化学工业出版社, 2005

[20] Huang X J, Liu Y, Cong R Z, et al. Chinese Journal of Chromatography, 2001, 19(4):293黄晓佳, 刘莺, 丛润滋, 等. 色谱, 2001, 19(4):293