doi:

DOI: 10.3724/SP.J.1123.2017.10025

Chinese Journal of Chromatography (色谱) 2018/36:2 PP.150-158

Determination of sedative and β-receptor hormone residues in feed by ultra high performance liquid chromatography-tandem mass spectrometry


Abstract:
An analysis and detection method for eight sedatives and fifteen β-receptor hormones in feed was established by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The sample was extracted with acetonitrile-1% (v/v) trichloroacetic acid aqueous solution (7:3, v/v), and purified by cationic solid phase extraction column. The extracts were separated on an Agilent Zorbax Eclipse Plus C18 chromatographic column (100 mm×3.0 mm, 1.8 μm) and then quantified via internal standard method. The results showed that the linear relationships of the 23 targets were good in the range of 2.0-200.0 μg/L (r2> 0.99). The average recoveries of the targets spiked in the matrix of feed samples were within 75.1%-102.4% in three spiked levels of 5.0, 10, 50 μg/kg, and the relative standard deviations (RSDs) were within 4.3%-14.3% (n=6). This method with high purification efficiency and extensive scope of application can be applied to screening and detecting sedatives and β -receptor hormones in feed.

Key words:cationic solid phase extraction column,ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS),sedative and β-receptor hormone,feed

ReleaseDate:2018-03-21 07:43:00



[1] Deng Y B. Hunan Agriculture, 1996(8):5邓云波. 湖南农业, 1996(8):5

[2] Ding H Z, Chen Z L, Yang Z L. Veterinary Pharmaceuticals & Feed Additives, 2002(1):18丁焕中, 陈杖榴, 杨志凌. 兽药与饲料添加剂, 2002(1):18

[3] Liu J, Liang G R, Li S H, et al. Journal of Food Safety & Quality, 2015, 6(4):1167刘佳, 梁桂荣, 李少晖, 等. 食品安全质量检测学报, 2015, 6(4):1167

[4] Wang P L. Quality and Safety of Agro-Products, 2014(1):44王培龙. 农产品质量与安全, 2014(1):44

[5] Bi Y F.[PhD Dissertation]. Beijing:China Agricultural University, 2014毕言锋.[博士学位论文]. 北京:中国农业大学, 2014

[6] Zhang Q L.[PhD Dissertation]. Nanchang:Nanchang University, 2011张秋兰.[博士学位论文]. 南昌:南昌大学, 2011

[7] Smith D J. J Anim Sci, 1998, 76(1):173

[8] Ministry of Agriculture of the People's Republic of China. The Maximum Residue Limit of Veterinary Drugs in Animal Origin Food. No. 235 Bulletin-2002 of the Ministry of Agriculture of the People's Republic of China. (2002-12-24). http://www.foodmate.net/law/shipin/163968.html中华人民共和国农业部. 动物性食品中兽药最高残留限量. 中华人民共和国农业部235号公告-2002. (2002-12-24). http://www.foodmate.net/law/shipin/163968.html

[9] Codex Alimentarius Commission (CAC). Codex Alimentarius Commission Maximum Residue Limits for Veterinary Drugs in Foods Updated as at the 35th Session of the Codex Alimentarius Commission (July 2012).[2017-10-25]. http://doc.mbalib.com/view/45cbdbed1ebc3d5acc6179e485c2ded0.html

[10] European Parliament and Council (EC). Regulation (EC) No. 470/2009 of the European Parliament and of the Council. (2009-05-06). http://down.foodmate.net/standard/sort/44/28727.html

[11] Wan Y P, Liu N, Tao G C, et al. Journal of Henan Agricultural Science, 2013, 42(2):132万宇平, 刘宁, 陶光灿, 等. 河南农业科学, 2013, 42(2):132

[12] Wang L, Li Y Q, Zhou Y K. Chinese Journal of Analysis Laboratory, 2009, 28(3):78王炼, 黎源倩, 周运柯. 分析试验室, 2009, 28(3):78

[13] Lu Y F, Xu L J, Wang H, et al. Journal of Chinese Mass Spectrometry Society, 2012, 33(5):280卢艳芬, 徐丽君, 王辉, 等. 质谱学报, 2012, 33(5):280

[14] Miyaguchi H, Kuwayama K, Tsujikawa K, et al. Forensic Sci Int, 2006, 157(1):57

[15] Liu Y P, Leng K L, Wang Q Y, et al. Marine Sciences, 2009, 33(4):34刘艳萍, 冷凯良, 王清印, 等. 海洋科学, 2009, 33(4):34

[16] Jia T, Liu H. Feed Research, 2014(3):55贾涛, 刘辉. 饲料研究, 2014(3):55

[17] Sun L, Liu Q, Zhang L, et al. Chinese Journal of Veterinary Drug, 2008(4):15孙雷, 刘琪, 张骊, 等. 中国兽药杂志, 2008(4):15

[18] De Wasch K, De Brabander H, Courtheyn D. Analyst (Cambridge, U. K.), 1998, 123(12):2270

[19] Juan C, Lgualada C, Moragues F, et al. J Chromatogr A, 2010, 1217(39):6061

[20] Wang N P, Ban F G, Peng L, et al. Journal of Chinese Mass Spectrometry Society, 2012, 33(2):94吴宁鹏, 班付国, 彭丽, 等. 质谱学报, 2012, 33(2):94

[21] Zheng L, Wu Y J, Zhao Y F, et al. Chinese Journal of Chromatography, 2014, 32(8):869郑玲, 吴玉杰, 赵永锋, 等. 色谱, 2014, 32(8):869

[22] GB/T 22286-2008

[23] Ministry of Agriculture of the People's Republic of China. No. 1025 Bulletin-18-2008 of the Ministry of Agriculture of the People's Republic of China. (2008-04-29). http://down.foodmate.net/standard/sort/9/17197.html中华人民共和国农业部. 中华人民共和国农业部1025号公告-18-2008. (2008-04-29). http://down.foodmate.net/standard/sort/9/17197.html

[24] Sun L, Zhang L, Xu Q, et al. Chinese Journal of Chromatography, 2010, 28(1):38孙雷, 张骊, 徐倩, 等. 色谱, 2010, 28(1):38

[25] Zeng H H, Li S Y, Feng Y J, et al. Journal of Medical Pest Control, 2009, 25(12):940曾红惠, 黎少映, 冯耀基, 等. 医学动物防制, 2009, 25(12):940

[26] Tan W, Zhang Y, Hong A H, et al. Chinese Journal of Spectroscopy Laboratory, 2013, 30(2):850谭微, 张毅, 洪爱华, 等. 光谱实验室, 2013, 30(2):850

[27] Liu X Y, Pan C P, Liu S. Chinese Journal of Pesticide Science, 2008(2):166刘新艳, 潘灿平, 刘肃. 农药学学报, 2008(2):166