DOI: 10.3724/SP.J.1123.2018.04024

Chinese Journal of Chromatography (色谱) 2018/36:9 PP.880-888

Determination of 42 veterinary drug residues in common animal derived food by dispersive solid phase extraction coupled with liquid chromatography-tandem mass spectrometry

A liquid chromatography-tandem mass spectrometry method, coupled with a dispersive solid phase extraction (DSPE) procedure for sample preparation, was developed to determine 13 classes of 42 veterinary drugs in four representative animal-derived foods. The analytes were dispersed with water, extracted with acetonitrile containing 5% (v/v) formic acid, salted out by salts, and purified by DSPE. The analytes were separated on a C18 column (100 mm×2.1 mm, 2 μ m) with gradient elution using the mobile phase containing methanol and 0.1% (v/v) formic acid aqueous solution. Electrospray ionization-mass spectrometry was performed in multiple reaction monitoring mode for analysis of the 42 compounds. The correlation coefficients of the standard calibration curves for the 42 veterinary drugs were all above 0.995. Most recoveries at three spiked levels in the four representative matrixes ranged from 65.8% to 135.5%, with relative standard deviations of 0.5%-14.2% (n=6). The limits of detection (LODs, S/N=3) and the limits of quantification (LOQs, S/N=10) were 0.01-1.68 μ g/kg and 0.01-5.62 μ g/kg, respectively. The method is simple, rapid, sensitive, and suitable for the simultaneous determination of the 42 veterinary drugs in animal-derived food.

Key words:dispersive solid phase extraction (dSPE),liquid chromatography-tandem mass spectrometry (LC-MS/MS),multi-classes of veterinary drug residues,animal derived food

ReleaseDate:2018-09-19 13:59:52

[1] Codex Alimentarius Commission. Maximum Residue Limits (MRLs) and Risk Management Recommendations (RMR) for Residuals of Veterinary Drugs in Foods.[2018-04-15].

[2] European Union. Commision Regulation (EU) No 37/2010 of 22 December 2009. (2009-12-22)[2018-04-15].

[3] Ministry of Agriculture. No. 235 Bulletin of the Ministry of Agriculture of the People's Republic of China. (2002-12-24)[2018-04-15]. 农业部. 中华人民共和国农业部公告第235号. (2002-12-24)[2008-06-29]

[4] Liu Z Y, Yang K, Chen F H, et al. Food Anal Methods, 2015, 8(2):352

[5] Chen D, Yu J, Tao Y, et al. J Chromatogr B, 2016, 1017/1018:82

[6] Robert C, Gillard N, Brasseur P Y, et al. Food Control, 2015, 50:509

[7] Luo H T, Xie M T, Huang X L, et al. Chinese Journal of Chromatography, 2015, 33(4):354 罗辉泰, 谢梦婷, 黄晓兰, 等. 色谱, 2015, 33(4):354

[8] Kaufmann A, Butcher P, Maden K, et al. Anal Chim Acta, 2007, 586(1/2):13

[9] Zhu W Y, Zhang X, Yang J, et al. Chinese Journal of Chromatography, 2015, 33(9):1002 朱万燕, 张欣, 杨娟, 等. 色谱, 2015, 33(9):1002

[10] Alcantara-Duran J, Moreno-Gonzalez D, Gilbert-Lopez B, et al. Food Chem, 2018, 245:29

[11] Cheng C, Liu L C. Anal Methods, 2014, 6(5):1581

[12] Li R, Yang L Q, Zhang P Y, et al. Chinese Journal of Chromatography, 2017, 35(12):1266 李蓉, 杨璐齐, 张鹏云, 等. 色谱, 2017, 35(12):1266

[13] Costi E M, Sicilia M D, Rubio S. J Chromatogr A, 2010, 1217(40):6250

[14] Guidi L R, Santos F A, Ribeiro A C S R, et al. Food Chem, 2018, 245:1232

[15] Huang Y, Zheng Z, Huang L, et al. J Pharm Biomed Anal, 2017, 138:363

[16] Mastrianni K R, Metavarayuth K, Brewer W E, et al. J Chromatogr B, 2018, 1084:64

[17] Dasenaki M E, Thomaidis N S. Anal Chim Acta, 2015, 880:103

[18] Guo D H, Deng X J, Zhao S J, et al. Chinese Journal of Analytical Chemistry, 2010, 38(3):318 郭德华, 邓晓军, 赵善贞, 等. 分析化学, 2010, 38(3):318

[19] Yoshikawa S, Nagano C, Kanda M, et al. J Chromatogr B, 2017, 1057:15

[20] Piatkowska M, Gbylik-Sikorska M, Gajda A, et al. Food Chem, 2017, 229:646

[21] Casado N, Perez-Quintanilla D, Morante-Zarcero S, et al. Talanta, 2017, 165:223

[22] Zhang Z, Li X, Ding S, et al. Food Chem, 2016, 204:252

[23] Jin Y, Zhang J, Zhao W, et al. Food Chem, 2017, 221:1298

[24] Zhou J, Xu J J, Cong J M, et al. J Chromatogr A, 2018, 1532:20

[25] Stubbings G, Bigwood T. Anal Chim Acta, 2009, 637(1/2):68

[26] Zhang K M, Liang F Y, Deng M, et al. Chinese Journal of Chromatography, 2016, 34(9):860 张科明, 梁飞燕, 邓鸣, 等. 色谱, 2016, 34(9):860

[27] Da J, Wang G L, Cao J, et al. Chinese Journal of Chromatography, 2015, 33(8):830 达晶, 王钢力, 曹进, 等. 色谱, 2015, 33(8):830

[28] Zhao L, Lucas D, Long D, et al. J Chromatogr A, 2018, 1549:14