DOI: 10.3724/SP.J.1123.2018.04012

Chinese Journal of Chromatography (色谱) 2018/36:9 PP.925-930

Analysis of fatty acid composition in cottonseed by gas chromatography with on-line pyrolytic methylation

A method of on-line pyrolytic methylation-gas chromatography was developed for the analysis of fatty acid composition in cottonseed. Fatty acids in cottonseeds were converted to their corresponding fatty acid methyl esters in the presence of trimethylsulfonium hydroxide at 300℃. The major fatty acids were linoleic acid (C18:2), oleic acid (C18:1), palmitic acid (C16:0), stearic acid (C18:0), myristic acid (C14:0), palmitoleic acid (C16:1), arachidic acid (C20:0) and docosanoic acid (C22:0). The unsaturated fatty acid content varied from 66.30% to 72.54%, and linoleic acid content varied from 43.20% to 53.61%. The RSDs of the peak areas of the fatty acids were less than 10% (n=5). The fatty acid compositions in the cottonseed samples, obtained from different growing places and different edible oil samples were compared by statistical analysis. The results showed that fatty acid compositions in cottonseed samples from different regions were similar. The fatty acid composition in cottonseed samples was closest to that of corn oil, and the similarity varied from 0.960 to 0.992. The method is simple, rapid, accurate, and is suitable for the analysis of fatty acid composition in cottonseed.

Key words:gas chromatography (GC),on-line pyrolytic methylation,fatty acids,cottonseed

ReleaseDate:2018-09-19 13:59:53

[1] Wang Y X, Liu Z J, Ma Z Y, et al. Cotton Science, 2011, 23(2):178 王彦霞, 刘正杰, 马峙英, 等. 棉花学报, 2011, 23(2):178

[2] Du W. China Oils and Fats, 2005, 30(1):37 杜玮. 中国油脂, 2005, 30(1):37

[3] Tang J Q, Wang Y P, Jiao Y. China Cotton Processing, 2009(4):32 唐金泉, 王毓蓬, 焦洋. 中国棉花加工, 2009(4):32

[4] Chen J S, Wang X Y, Hu E Z, et al. Transactions of the Chinese Society of Agricultural Engineering, 2010, 26(1):301 陈金思, 汪向洋, 胡恩柱, 等. 农业工程学报, 2010, 26(1):301

[5] Joshi H, Moser B R, Walker T. J Am Oil Chem Soc, 2012, 89(1):145

[6] Shang L G, Li J H, Wang Y M, et al. Spectroscopy and Spectral Analysis, 2015, 35(3):609 商连光, 李军会, 王玉美, 等. 光谱学与光谱分析, 2015, 35(3):609

[7] Hui R H, Hou D Y, Li T C, et al. Journal of Chinese Mass Spectrometry Society, 2005, 26(2):90 回瑞华, 侯冬岩, 李铁纯, 等. 质谱学报, 2005, 26(2):90

[8] Tian X H, Xie C X. Food Science and Technology, 2012, 37(10):154 田小红, 解成喜. 食品科技, 2012, 37(10):154

[9] Kossa W C, MacGee J, Ramachandran S, et al. J Chromatogr Sci, 1979, 17(4):177

[10] Fu Y F, Zhang P P, Pan Z F, et al. Chinese Journal of Analysis Laboratory, 2013, 32(8):103 傅宇飞, 张佩佩, 潘再法, 等. 分析试验室, 2013, 32(8):103

[11] Ishida Y, Katagiri M, Ohtani H. J Chromatogr A, 2009, 1216(15):3296

[12] Wang M X, Zhou D Y, Ma L, et al. Food Science, 2016, 37(22):136 王美霞, 周大云, 马磊, 等. 食品科学, 2016, 37(22):136

[13] Lauritzen I, Blondeau N, Heurteaux C, et al. EMBO J, 2000, 19(8):1784

[14] Qureshi M N. J Chin Chem Soc, 2012, 59(1):46

[15] Liu Z J, Guo B S, Zhang Y, et al. Molecular Plant Breeding, 2012, 10(6):1038 刘正杰, 郭宝生, 张园, 等. 分子植物育种, 2012, 10(6):1038