DOI: 10.3724/SP.J.1123.2018.04003

Chinese Journal of Chromatography (色谱) 2018/36:9 PP.931-937

Uniform and orthogonal designs on experimental design and optimization of micellar electrokinetic capillary chromatographic method for the simultaneous analysis of three nucleoside antivirus drugs in disinfectant and anti/bacteriostatic products

To prevent the abuse of nucleoside-based antiviral drugs in disinfectant and anti/bacteriostatic products, a new micellar electrokinetic chromatographic (MEKC) method for the simultaneous determination of three nucleoside antiviral drugs (ganciclovir, acyclovir and penciclovir) was established. Three factors and seven levels uniform design and four factors and four levels orthogonal design were used to optimize the concentration of sodium dodecyl sulfate (SDS), sodium dihydrogen phosphate (NaH2PO4) and sodium borate (Na2B4O7) in the running buffer. The three nucleoside antiviral drugs were separated from each other in the shortest time to obtain effective separation through uniform and orthogonal designs on experimental design and optimization. The separation was performed on an uncoated fused-silica capillary with 50 μ m i. d. and a total length of 30.2 cm (effective length:20 cm). An optimized buffer solution consisting of 25 mmol/L NaH2PO4, 10 mmol/L Na2B4O7 (pH 7.41) and 140 mmol/L dodecyl sulfate sodium was used for separation. The applied voltage was 10 kV, the injected pressure and time was at 0.003 Pa for 4 s. The wavelength of the detection was 250 nm. Under the optimum conditions, the corrected area and the concentration had good linearity. The correlation coefficients (r) were not less than 0.9995. The limits of detection were all 2.0 mg/kg. The limits of quantitation were all 7.0 mg/kg. The relative recoveries ranging from 85.4% to 104.1% with RSDs all lower than 8.0%. It showed that the method was suitable for the detection of ganciclovir, acyclovir and penciclovir in disinfectant and anti/bacteriostat products with simplicity and rapidity.

Key words:micellar electrokinetic chromatography (MEKC),uniform experimental design,orthogonal experimental design,nucleoside antiviral drugs,disinfectant,anti/bacteriostatic products

ReleaseDate:2018-09-19 13:59:53

[1] Wu J B, Lin L. Journal of Clinical Dermatology, 2006, 35(1):57 吴建兵, 林麟. 临床皮肤科杂志, 2006, 35(1):57

[2] Li J Y. China Pharmaceuticals, 2008, 17(16):75 李金英. 中国药业, 2008, 17(16):75

[3] Li G H. China Journal of Pharmaceutical Economics, 2016, 11(9):24 李桂华. 中国药物经济学, 2016, 11(9):24

[4] Long X Y, Deng M, Chen F N. Journal of Guizhou Normal University (Natural Sciences), 2013, 31(6):73 龙星宇, 邓茂, 陈福南. 贵州师范大学学报(自然科学版), 2013, 31(6):73

[5] Xie A L, Wang W. Strait Pharmaceutical Journal, 2013, 25(4):41 谢爱丽, 王维. 海峡药学, 2013, 25(4):41

[6] Sun L H, Dong J, Miao L Y. The Chinese Journal of Clinical Pharmacology, 2013, 29(3):219 孙丽红, 董吉, 缪丽燕. 中国临床药理学杂志, 2013, 29(3):219

[7] Kong A Y, Ji H, Han J, et al. Chinese Journal of Antibiotics, 2015, 40(8):607 孔爱英, 纪宏, 韩杰, 等. 中国抗生素杂志, 2015, 40(8):607

[8] Saleh S, Hempel G. Electrophoresis, 2006, 27(12):2439

[9] Yeh H H, Yang Y H, Chen S H. Electrophoresis, 2006, 27(4):819

[10] Vo H C, Henning P A, Leung D T, et al. J Chromatogr B Analyt Technol Biomed Life Sci, 2002, 772(2):291

[11] Liang G L, Lian Y. West China Journal of Pharmaceutical Sciences, 2006, 21(3):286 梁改玲, 连莹. 华西药学杂志, 2006, 21(3):286

[12] Neubert R H H, Mrestani Y, Schwarz M, et al. J Pharm Biomed Anal, 1998, 16(5):893

[13] Huang X J, Gao Q, Zhu X Y, et al. Chinese Pharmaceutical Journal, 2014, 49(5):419 黄晓君, 高青, 朱晓月, 等. 中国药学杂志, 2014, 49(5):419

[14] Zhang R L, Xu J H, Huang W B. Journal of Jiangxi University of Traditional Chinese Medicine, 2015, 27(6):72 张若良, 许江红, 黄伟波. 江西中医药大学学报, 2015, 27(6):72

[15] Chen M Q, Wang C L, Lin C H. Strait Pharmaceutical Journal, 2017, 29(1):204 陈美钦, 王长连, 林翠鸿. 海峡药学, 2017, 29(1):204

[16] Rigo-Bonnin R, Padulles A, Corral-Comesana S, et al. Clin Chim Acta, 2014, 427(1):58

[17] Huang Z D. Chinese Pharmaceutical Journal, 2000, 35(2):51 黄志东. 中国药学杂志, 2000, 35(2):51

[18] Wu H F, Guan F Y, Luo Y. Chinese Journal of Analytical Chemistry, 1996, 24(10):1117 吴惠芳, 关福玉, 罗毅. 分析化学, 1996, 24(10):1117

[19] Fu X S, Guo W W, Jiang Z, et al. Journal of Shenyang Pharmaceutical University, 2013, 30(5):351 付小帅, 郭雯雯, 姜珍, 等. 沈阳药科大学学报, 2013, 30(5):351

[20] Guo W W, Fu X S, Cui Y, et al. Journal of Shenyang Pharmaceutical University, 2013, 30(4):281 郭雯雯, 付小帅, 崔艳, 等. 沈阳药科大学学报, 2013, 30(4):281

[21] Zhu H B, Xi K Q. Journal of Capital University of Physical Education and Sports, 2013, 25(3):283 朱红兵, 席凯强. 首都体育学院学报, 2013, 25(3):283