doi:

DOI: 10.3724/SP.J.1123.2018.04008

Chinese Journal of Chromatography (色谱) 2018/36:9 PP.938-945

Simultaneous determination of 77 pesticide residues in eight kinds of herbal tea by liquid chromatography-tandem mass spectrometry


Abstract:
An effective method was developed for the determination of 77 pesticide residues in eight kinds of herbal tea by modified QuEChERS and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The target compounds were extracted with 1% (v/v) acetic acid-acetonitrile and 1 g ammonium acetate, and purified by 4 g MgSO4, 0.05 g C18, 0.50 g primary secondary amine (PSA) sorbent and 0.05 g graphitized carbon black (GCB). The 0.1% (v/v) formic acid aqueous solution and acetonitrile were used as the mobile phases. The extracts were separated on a Venusil MP C18 chromatographic column using a positive electrospray ionization (ESI) and negative ESI source under the selected ion monitoring (SIM) mode. The analytes were quantified by the matrix match standard solutions. The good linearities were obtained in the range of 0.5-100.0 μ g/L, and the correlation coefficients were all greater than 0.995. The average recoveries of the 77 pesticide residues ranged from 70.3% to 110.0%, and RSDs were in the range of 2.6%-9.8%. The limits of detection were 1.0-10.0 μ g/kg. The method is sensitive, accurate and precise, and can meet the requirements for the determination of pesticide residues.

Key words:high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),QuEChERS,pesticide residues,herbal tea

ReleaseDate:2018-09-19 13:59:53



[1] NY/T 1506-2015

[2] Payá P, Anastassiades M, Mack D, et al. Anal Bioanal Chem, 2007, 389(6):1697

[3] Mei W Q, Li Q W, Zou Y H, et al. Journal of Agro-Environment Science, 2007, 26(5):1832 梅文泉, 黎其万, 邹艳虹, 等. 农业环境科学学报, 2007, 26(5):1832

[4] Xiang Z X, Zhao W J, Guo Q S. China Journal of Chinese Meteria Medica, 2006, 31(16):1321 向增旭, 赵维佳, 郭巧生. 中国中药杂志, 2006, 31(16):1321

[5] Lu H X, Li J R, Xia H L. Journal of Chinese Institute of Food Science and Technology, 2007, 7(3):18 陆海霞, 励建荣, 夏会龙. 中国食品学报, 2007, 7(3):18

[6] Sun W J, Gao F, Wei S M, et al. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2004, 33(3):343 孙威江, 高峰, 危赛明, 等. 福建农林大学学报(自然科学版), 2004, 33(3):343

[7] Zhang Y L, Ni L. Strait Journal of Preventive Medicine, 2013, 19(4):53 张云琳, 倪蕾. 海峡预防医学杂志, 2013, 19(4):53

[8] Duan L C, Xu F, Xia Q H, et al. Modern Preventive Medicine, 2016, 43(20):3780 段丽村, 徐芳, 夏清海, 等. 现代预防医学, 2016, 43(20):3780

[9] Yan H F, Wang M L, Chen L, et al. Chemical Analysis and Meterage, 2016, 25(6):62 颜鸿飞, 王美玲, 陈练, 等. 化学分析计量, 2016, 25(6):62

[10] Sun L, Jin H Y, Wang Y. Journal of Pharmaceutical Analysis, 2012(11):2017 孙磊, 金红宇, 王莹, 等. 药物分析杂志, 2012(11):2017

[11] Zhao W L, Zhu M, Chen B L, et al. Journal of Pharmaceutical Analysis, 2014(3):447 赵维良, 祝明, 陈碧莲, 等. 药物分析杂志, 2014(3):447

[12] Wang L Z, Zhou Y, Huang X Y, et al. Chinese Journal of Chromatography, 2013, 31(12):1167 王连珠, 周昱, 黄小燕, 等. 色谱, 2013, 31(12):1167

[13] Liu S N, Zhao X Y, Dong X Q, et al. Chinese Journal of Chromatography, 2015, 33(11):1205 刘松南, 赵新颖, 董晓倩, 等. 色谱, 2015, 33(11):1205

[14] Zhu L, Chen H P, Zhou S J, et al. Chinese Journal of Analytical Chemistry, 2015, 43(2):271 诸力, 陈红平, 周苏娟, 等. 分析化学, 2015, 43(2):271

[15] Sun M Y, Shi Z H, Li J X, et al. Journal of Instrumental Analysis, 2017, 36(5):595 孙梦园, 石志红, 李建勋, 等. 分析测试学报, 2017, 36(5):595

[16] Zhu L, Wang C, Chen H P, et al. Chinese Journal of Analytical Chemistry, 2017, 45(4):529 诸力, 王晨, 陈红平, 等. 分析化学, 2017, 45(4):529

[17] Zhou L, Luo F J, Zhang X Z, et al. Journal of Instrumental Analysis, 2014, 33(6):642 周利, 罗逢健, 张新忠, 等. 分析测试学报, 2014, 33(6):642

[18] Chen L, Shangguan L M, Fu F F. Scientia Sinica Chimica, 2016, 46(3):302 陈磊, 上官良敏, 付凤富. 中国科学:化学, 2016, 46(3):302

[19] Sun D, Fu B N, Zhang Z G. The Food Industry, 2016, 37(7):243 孙迪, 付本宁, 张志国. 食品工业, 2016, 37(7):243

[20] Lai L L. Journal of Food Safety & Quality, 2018, 9(2):306 赖凌凌. 食品安全质量检测学报, 2018, 9(2):306

[21] Yin W, Song Z R, Liu J Q, et al. China Journal of Chinese Materia Medica, 2015, 40(4):679 尹伟, 宋祖荣, 刘金旗, 等. 中国中药杂志, 2015, 40(4):679

[22] Li X S, Xu G K, Wang G K, et al. Chinese Wild Plant Resources, 2015, 34(3):42 李旭森, 许光凯, 王国凯, 等. 中国野生植物资源, 2015, 34(3):42

[23] Ni F Y, Wen J H, Li M, et al. Chinese Traditional and Herbal Drugs, 2017, 48(18):3689 倪付勇, 温建辉, 李明, 等. 中草药, 2017, 48(18):3689

[24] Lehotay S J, Kok A D, Hiemstram M, et al. J AOAC Int, 2005, 88(2):595

[25] Lehotay S J, Mastovska K, Lighfield A R. J AOAC Int, 2005, 88(2):615

[26] Pay P, Anastasslades M, Mack D, et al. Anal Bioanal Chem, 2007, 389:1697

[27] Yue T Y, Fan C B, Zhou Y M, et al. Science and Technology of Tianjin Agriculture and Forestry, 2005(5):17 岳天宇, 范春斌, 周艳明, 等. 天津农林科技, 2005(5):17

[28] Dong J, Gong X M, Zhang L, et al. Chinese Journal of Analysis Laboratory, 2008, 27(3):91 董静, 宫小明, 张立, 等. 分析试验室, 2008, 27(3):91

[29] Agilent Technology. Enviromental Chemistry, 2005, 24(4):486 安捷伦科技. 环境化学, 2005, 24(4):486

[30] Wang S W, Liu Y P, Sun H B, et al. Chinese Journal of Chromatography, 2018, 36(1):17 王思威, 刘艳萍, 孙海滨, 等. 色谱, 2018, 36(1):17

[31] Chu N M, Meng X, Kang Y Q, et al. Food Science, 2016, 37(24):239 褚能明, 孟霞, 康月琼, 等. 食品科学, 2016, 37(24):239

[32] Wei Q, Wang Y H, Hu Y Y, et al. Journal of Northwest Forestry University, 2005(3):140 尉芹, 王永红, 胡亚云, 等. 西北林学院学报, 2005(3):140

[33] Agilent Technology. GC-MS Analysis of Pesticide Residues in Spinach was Determined by Agilent Bond Elut QuEChERS AOAC Kit. (2012-01-06)[2018-01-06]. https://www.agilent.com/chem/cn 安捷伦科技. 用Agilent Bond Elut QuEChERS AOAC试剂盒测定菠菜中农药残留的GC-MS分析. (2012-01-06)[2018-01-06]. https://www.agilent.com/chem/cn