DOI: 10.3724/SP.J.1123.2018.01024

Chinese Journal of Chromatography (色谱) 2018/36:7 PP.621-628

Simultaneous determination of 18 food-borne stimulant drug residues in beef using ultra-high performance liquid chromatography-tandem mass spectrometry

A method for the simultaneous determination of 18 food-borne stimulant drug residues in beef was developed based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The beef sample was extracted with acidified acetonitrile, cleaned up by Captiva filtration cartridge. The extract was dried with anhydrous magnesium sulfate, and then concentrated by nitrogen flow. The obtained residue was re-dissolved in methanol-water (7:3, v/v). The separation was performed on an Agilent Zorbax Phenyl-Hexyl column with 5 mmol/L ammonium acetate solution (containing 0.01% (v/v) acetic acid) and methanol-acetonitrile (7:3, v/v) as mobile phases with gradient elution. The analyte was detected in positive and negative ion modes and the multiple reaction monitoring (MRM) mode. The quantification analysis was performed by external standard method using matrix-matched calibration curves. The method was linear with the correlation coefficients (R2) ≥ 0.9950 in the range of 0.10-50 μg/L. At the spiked levels of 0.4, 1.0 and 2.0 μg/kg, the recoveries of all compounds ranged from 57.3% to 117.5%, with RSDs in range of 3.1%-15.6% (n=5). The limits of detection and limits of quantification were in the range of 0.0006-0.0900 μg/kg and 0.0020-0.3000 μg/kg, respectively. The method is simple, rapid, accurate and sensitive, and can meet the requirement for the determination of the 18 food-borne stimulant drug residues in beef.

Key words:ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS),food-borne stimulant drug,residues,beef tissue

ReleaseDate:2018-09-19 18:37:18

[1] Wang H L, Lu R, Wu Y. Liaoning Sport Science and Technology, 2013, 35(6):36 王洪利, 路睿, 武勇. 辽宁体育科技, 2013, 35(6):36

[2] Ministry of Agriculture. No. 235 Bulletin of the Ministry of Agriculture of the People's Republic of China. (2002-12-24)[2017-12-11]. 农业部. 中华人民共和国农业部公告第235号. (2002-12-24)[2017-12-11].

[3] Ma L L, Liu Y, Jia L, et al. Journal of Food Safety and Quality, 2013, 4(3):847 马立利, 刘艳, 贾丽, 等. 食品安全质量检测学报, 2013, 4(3):847

[4] He F Y, Xi R M, Wan Y P, et al. Science and Technology Innovation Herald, 2016, 13(2):165 何方洋, 郗日沫, 万宇平, 等. 科技创新导报, 2016, 13(2):165

[5] Iglesias Y, Fente C A, Vazquez B, et al. J Agric Food Chem, 1999, 47(10):4275  

[6] Chen J, Qin Y, Zhang M J. Chinese Journal of Chromatography, 2006, 24(1):19 陈捷, 秦燕, 张美金. 色谱, 2006, 24(1):19

[7] Zeng D P, Lin C P, Zeng Z L, et al. Scientia Agricultura Sinica, 2008, 41(9):2776 曾东平, 林翠萍, 曾振灵, 等. 中国农业科学, 2008, 41(9):2776

[8] Lu J, Feng X M, Sui X, et al. Chinese Journal of Analytical Chemistry, 2013, 41(10):1487 卢杰, 冯晓梅, 隋晓, 等. 分析化学, 2013, 41(10):1487  

[9] Krone N, Hughes B A, Lavery G G, et al. J Steroid Biochem, 2010, 121(3/5):496

[10] Liu G H, Qin W, Liu H H, et al. Journal of Hygiene Research, 2013, 42(1):61 刘桂华, 秦伟, 刘红河, 等. 卫生研究, 2013, 42(1):61

[11] Shao B, Zhao R, Meng J, et al. Anal Chim Acta, 2005, 548(1/2):41

[12] Blasco C, Poucke C V, Peteghem C V. J Chromatogr A, 2007, 1154(1/2):230

[13] Yang Y, Shao B, Zhang J, et al. J Chromatogr B, 2009, 877(5/6):489

[14] Cai Q R, Feng J W, Zhang Y, et al. Chinese Journal of Chromatography, 2011, 29(8):712 蔡勤仁, 冯家望, 张毅, 等. 色谱, 2011, 29(8):712

[15] Xu J Z, Ding T, Liu F. China Food, 2012(6):62 徐锦忠, 丁涛, 刘飞. 中国食品, 2012(6):62

[16] Wan C H, Zhang Y W, Huang Y S, et al. Modern Scientific Instruments, 2016(5):83 万春花, 张云伟, 黄优生, 等. 现代科学仪器, 2016(5):83

[17] Di D L, Benabdelkamel H, Taverna D, et al. Anal Bioanal Chem, 2015, 407(19):5835  

[18] Xu C L, Chu X G, Peng C F, et al. J Pharm Biomed Anal, 2006, 41(2):616  

[19] Lian Y J, Lin S H, Zeng Q, et al. Journal of Food Safety and Quality, 2014, 5(2):384 连英杰, 林升航, 曾琪, 等. 食品安全质量检测学报, 2014, 5(2):384

[20] Wang G, Sun Y Y, Tang X Z, et al. Science and Technology of Food Industry, 2014, 35(23):282 王刚, 孙媛媛, 唐晓姝, 等. 食品工业科技, 2014, 35(23):282

[21] Ehling S, Reddy T M. J Agric Food Chem, 2013, 61(48):11782  

[22] Kudo K, Usumoto Y, Usui K, et al. Forensic Toxicol, 2014, 32(1):97