doi:

DOI: 10.3724/SP.J.1123.2018.08040

Chinese Journal of Chromatography (色谱) 2018/36:12 PP.1290-1296

Analysis of methamphetamine and amphetamine chiral enantiomers in hair by high performance liquid chromatography-tandem mass spectrometry


Abstract:
A chiral separation method was developed for the analysis of methamphetamine (METH) and amphetamine (AM) in hair by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were extracted using methanol by ultrasonication in a high-temperature water bath. The chromatographic separation was performed on a SUPELCO Astec CHIROIOTIC® V2 column with the mobile phase of methanol-20 mmol/L ammonium acetate aqueous solution containing 0.1% (v/v) formic acid (99:1, v/v). All the compounds showed linear relationship in the range of 15-300 ng/mg (r>0.99). The limits of detection (LODs) of METH and AM were 0.1 ng/mg and 0.15 ng/mg, respectively. The limits of quantification (LOQs) of METH and AM were 0.4 ng/mg and 0.5 ng/mg, respectively. The inter-day precisions were all ≤ 6.8%, and the intra-day precisions were all ≤ 11.4%. Fifty drug abuse samples were determined by this method. Up to 70% of the cases were determined as S-(+)-METH and S-(+)-AM, while 18% of the cases were S-(+)-METH, S-(+)-AM, R-(-)-METH and R-(-)-AM. The proposed method is rapid, simple, and provides technical support and a scientific basis for the chiral analysis in actual hair samples for forensic toxicant identification.

Key words:high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),enantiomers,methamphetamine (METH),amphetamine (AM),hair

ReleaseDate:2019-01-03 10:27:22



[1] Xu Y Y, Zhuang H. Chinese Journal of Drug Abuse Prevention and Treatment, 2010, 16(1):22 徐媛媛, 庄华. 中国药物滥用防治杂志, 2010, 16(1):22

[2] An M L, Ju A H, Guan J L. Journal of Yunnan Police College, 2016(6):7 安美玲, 巨爱焕, 关江林. 云南警官学院学报, 2016(6):7

[3] Xiang P, Bu J, Qiao Z, et al. Journal of Forensic Medicine, 2017, 33(6):599 向平, 卜俊, 乔正, 等. 法医学杂志, 2017, 33(6):599

[4] Xiao R S, Huang P, Li J W, et al. Chinese Journal of Drug Abuse Prevention and Treatment, 2017, 23(2):85 肖瑞森, 黄平, 李津伟, 等. 中国药物滥用防治杂志, 2017, 23(2):85

[5] Yue Z J, Cheng T T. Science and Technology, 2016, 26(27):282 岳宗杰, 程婷婷. 科技展望, 2016, 26(27):282

[6] Suwannachom N, Thananchai T, Junkuy A, et al. Forensic Sci Int, 2015, 254:80

[7] Zhang Y H. Chinese Journal of Drug Abuse Prevention and Treatment, 2013, 19(6):346 张亚海. 中国药物滥用防治杂志, 2013, 19(6):346

[8] Shen M, Xiang P, Shen B H, et al. Chinese Journal of Chromatography, 2008, 26(4):454 沈敏, 向平, 沈保华, 等. 色谱, 2008, 26(4):454

[9] Xiang P, Shen M, Shen B H, et al. Chinese Journal of Chromatography, 2008, 26(4):469 向平, 沈敏, 沈保华, 等. 色谱, 2008, 26(4):469

[10] Leung K W, Wong Z C F, Ho J Y M, et al. Drug Test Anal, 2018, 10(6):977

[11] Shu I, Alexander A, Jones M, et al. J Chromatogr B, 2016, 1028:145

[12] Wang T, Shen B H, Shi Y, et al. Forensic Sci Int, 2015, 246:72

[13] Zhu D, Meng P H, He H Y. Chinese Journal of Chromatography, 2007, 25(1):16 朱丹, 孟品佳, 何洪源. 色谱, 2007, 25(1):16

[14] Borg D, Kolb E, Lantigua C, et al. J Anal Toxicol, 2017, 42(1):1

[15] Wang T, Shen B H, Wu H J, et al. Forensic Sci Int, 2018, 284:153

[16] Zeng S. Chinese Journal of Forensic Medicine, 1994, 9(1):25 曾苏. 中国法医学杂志, 1994, 9(1):25

[17] Shen M, Shen B H. Chinese Journal of Pharmaceutical Analysis, 1998, 18(1):21 沈敏, 沈保华. 药物分析杂志, 1998, 18(1):21

[18] Chen J, Shi H. Chinese Journal of Chromatography, 2017, 35(12):1229 陈娇, 石浩. 色谱, 2017, 35(12):1229

[19] Dai R J, Wang H T, Sun W W, et al. Chinese Journal of Chromatography, 2016, 34(1):34 戴荣继, 王慧婷, 孙维维, 等. 色谱, 2016, 34(1):34