doi:

DOI: 10.3724/SP.J.1123.2018.08008

Chinese Journal of Chromatography (色谱) 2018/36:12 PP.1330-1336

Simultaneous determination of nine mycotoxins in cereal and cereal products by high performance liquid chromatography with composite immunoaffinity clean-up column


Abstract:
A rapid analytical method using high performance liquid chromatography (HPLC) with post-column derivatization was established for the simultaneous determination of nine mycotoxins in cereal and cereal products. The nine mycotoxins were extracted from the samples by using acetone-water (80:20, v/v). The extract was purified by a self-made composite immunoaffinity clean-up column and was analyzed by HPLC. Mycotoxins were detected by fluorescence detection after on-line photochemical derivatization and were quantified by an external standard method. The nine compounds showed good linearities in their concentration ranges with the correlation coefficients (R2) exceeding 0.999. The spiked recoveries in real samples for the nine mycotoxins were above 80%, while the relative standard deviations (RSDs) were in the range of 1.0%-5.6%. The limits of detection (LODs) and the limits of quantification (LOQs) were 0.02-5.00 and 0.07-16.70 μg/kg, respectively. The developed method is simple, sensitive, and has good repeatability, and thus can be used for the routine analysis of the nine mycotoxins in cereal and cereal products.

Key words:high performance liquid chromatography (HPLC),composite immunoaffinity column,mycotoxin,cereal and cereal products

ReleaseDate:2019-01-03 10:27:25



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