doi:

DOI: 10.3724/SP.J.1123.2018.08043

Chinese Journal of Chromatography (色谱) 2019/37:1 PP.15-20

Rapid untargeted screening and determination of unknown contaminants in aquatic products by high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry


Abstract:
A method has been developed for rapid untargeted screening and determination of unknown contaminants in aquatic products by high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry. The samples were extracted by acetonitrile, dried under nitrogen, dissolved in methanol-water (3:7, v/v), and analyzed via the full MS scan/data dependent MS2 mode during the screening process. The Trace Finder software was used to match the precise mass, the isotope abundance ratio, the fragment ion to search for unknown contaminants in aquatic samples. The optimized QuEChERS method is used to purify the samples when quantifying. The quantitative analyses of triazole, caffeine, and ethoxyquinoline were performed via the target-MS2 mode. The correlation coefficients of the three compounds in fish and shrimp samples were higher than 0.99 in the linear range of 5-1000 μg/L. The limit of detection was 1 μg/kg, and the limit of quantitation was 5 μg/kg. The average recoveries were between 70.5% and 90.9% with the relative standard deviations ranging from 5.4% to 12.8%. The screening method has the advantages of fast, accurate, and high throughput; when combined with the quantitative method, it can be used to screen and determine unknown contaminants in the actual aquatic products.

Key words:high performance liquid chromatography (HPLC),high resolution mass spectrometry (HRMS),untargeted screening,aquatic products,contaminants

ReleaseDate:2019-01-31 09:03:36



[1] Zhang H, Sun C. Research of Agricultural Modernization, 2009, 30(4):430 张欢, 孙琛. 农业现代化研究, 2009, 30(4):430

[2] Zhao L, Geng R, Ouyang H Y. World Agriculture, 2015(5):121 赵蕾, 耿瑞, 欧阳海鹰. 世界农业, 2015(5):121

[3] Yang X L, Guo W W, Sun Q. Chinese Fishery Quality and Standards, 2013, 3(4):1 杨先乐, 郭微微, 孙琪. 中国渔业质量与标准, 2013, 3(4):1

[4] China Food and Drug Administration. Notice of the General Administration on Special Inspection Results of Key Aquatic Products in Operation (No. 34 of 2017). (2017-02-24)[2017-02-25]. http://news.foodmate.net/2017/02/418886.html 国家食药监总局. 总局关于经营环节重点水产品专项检查结果的通告(2017年第34号). (2017-02-24)[2017-02-25]. http://news.foodmate.net/2017/02/418886.html

[5] China Food and Drug Administration. Notice of the General Administration on Monitoring Results of fresh Aquatic Products in Operation (No. 176 of 2017). (2017-11-02)[2017-11-09]. http://news.foodmate.net/2017/11/449104.html 国家食品药品监督管理总局. 总局关于经营环节鲜活水产品抽检监测结果的通告(2017年第176号). (2017-11-02)[2017-11-09]. http://news.foodmate.net/2017/11/449104.html

[6] Chen S J, Li L H, Yang X Q, et al. Journal of Food Science, 2015, 36(17):300 陈胜军, 李来好, 杨贤庆, 等. 食品科学, 2015, 36(17):300

[7] Song C, Hu G D, Meng S L, et al. Journal of Agriculture, 2017, 7(9):58 宋超, 胡庚东, 孟顺龙, 等. 农学学报, 2017, 7(9):58

[8] Lü X M. Environmental Monitoring in China, 2012, 28(4):118 吕小明. 中国环境监测, 2012, 28(4):118

[9] Zhou X J, Chen Y, Yang S J, et al. Chinese Journal of Chromatography, 2017, 35(8):787 周秀锦, 陈宇, 杨赛军, 等. 色谱, 2017, 35(8):787

[10] Xu Y Y, Zhang D, Qiu J, et al. Journal of Quality and Safety of Agro-Products, 2016(5):13 许彦阳, 张丹, 邱静, 等. 农产品质量与安全, 2016(5):13

[11] Wu B, Ding T, Liu H, et al. Chinese Journal of Chromatography, 2012, 30(12):1246 吴斌, 丁涛, 柳菡, 等. 色谱, 2012, 30(12):1246

[12] Krauss M, Singer H, Hollender J. Anal Bioanal Chem, 2010, 397(3):943

[13] Ondarza P M, Gonzalez M, Fillmann G, et al. Chemosphere, 2014, 94(1):135

[14] Chiesa L M, Labella G F, Panseri S, et al. Chemosphere, 2016, 153:162

[15] Nacher-Mestre J, Serrano R, Portoles T, et al. J Agric Food Chem, 2014, 62(10):2165

[16] Tengstrand E, Rosén J, Hellenas K E, et al. Anal Bioanal Chem, 2013, 405(4):1237

[17] Shi Y F, Cai Y Q, Yu H J, et al. Chinese Journal of Analytical Chemistry, 2014, 42(11):1640 史永富, 蔡友琼, 于慧娟, 等. 分析化学, 2014, 42(11):1640

[18] Mao T, Lu Y, Jiang J, et al. Journal of Food Science, 2016, 37(5):245 毛婷, 路勇, 姜洁, 等. 食品科学, 2016, 37(5):245

[19] Meng D, Tan Z J, Liu Y T, et al. Chinese Agricultural Science Bulletin, 2015, 31(14):56 孟娣, 谭志军, 刘永涛, 等. 中国农学通报, 2015, 31(14):56

[20] Zhang P W, Zhou H D, Zhao G F, et al. Environmental Science, 2016, 37(9):3348 张盼伟, 周怀东, 赵高峰, 等. 环境科学, 2016, 37(9):3348

[21] Zhang P W, Zhou H D, Zhao G F, et al. Environmental Science, 2017, 38(5):1852 张盼伟, 周怀东, 赵高峰, 等. 环境科学, 2017, 38(5):1852

[22] GB 2763-2016