doi:

DOI: 10.3724/SP.J.1123.2018.08013

Chinese Journal of Chromatography (色谱) 2019/37:1 PP.87-92

Non-targeted screening and determination of steviol glycosides in Chinese liquor and beverages by ultra high performance liquid chromatography-quadrupole/Orbitrap mass spectrometry


Abstract:
A method for simultaneous screening and determination of steviol glycosides in Chinese liquor and beverages by hydrophilic interaction ultra-high performance liquid chromatography-quadrupole/Orbitrap high resolution mass spectrometry (HILIC-UHPLC-Q/Orbitrap HRMS) was developed. In addition, the MS fragment path ways of steviol glycosides were characterized. The sample was diluted and filtrated before analysis, separated by a Waters Xbridge Amide column (150 mm×4.6 mm, 3.5 μm), and determined via UHPLC-Q/Orbitrap HRMS. The analytes were electronic using electro spray ion source in the negative mode, and the data were collected using full mass scan and data-dependent MS2 scan. Good linear relationships (10-1000 μg/L) were obtained. The limits of detection ranged from 0.3 to 20 μg/L. The mean recoveries varied from 81.9% to 106% at the spiked levels of 10-100 μg/kg, and the relative standard deviations ranged from 0.1% to 9.3% (n=3). Two characteristic fragment ions of steviol glycosides were identified by the analysis of fragment ion spectra of eight types of steviol glycosides standards. Non-targeted screening of unknown steviol glycosides in samples was carried out on the basis of the identified characteristic fragment ions. The proposed method is comprehensive, accurate, and sensitive for rapid screening and determination of sweeteners in food samples.

Key words:ultra high performance liquid chromatography (UHPLC),high resolution mass spectrometry (HRMS),non-targeted screening,steviol glycosides

ReleaseDate:2019-01-31 09:03:38



[1] Dusek J, Carazo A, Trejtnar F, et al. Food Chem Toxicol, 2017, 109:130

[2] Shao H J, Hu Y G, Shen M S, et al. Chinese Journal of Analytical Chemistry, 2002, 30(9):1152 邵寒娟, 胡涌刚, 沈明山, 等. 分析化学, 2002, 30(9):1152

[3] Gallo M, Ferranti P. J Chromatogr A, 2016, 1428:3

[4] Jiang Y X, Wei R X, Yang G Z, et al. Journal of Instrumental Analysis, 2009, 28(7):838 蒋奕修, 魏瑞霞, 杨桂珍, 等. 分析测试学报, 2009, 28(7):838

[5] Liang T, Fu Q, Shen A, et al. J Chromatogr A, 2015, 1388:110

[6] Jamaluddin A M, Smith R M. J Sep Sci, 2002, 25(3):170

[7] Londhe S V, Nanaware S M. J AOAC Int, 2013, 96(1):24

[8] Gu Y, Sheng Y G, Han L, et al. Chinese Journal of Analysis Laboratory, 2014, 33(7):796 顾莹, 盛永刚, 韩丽, 等. 分析试验室, 2014, 33(7):796

[9] Donna D L, Mazzotti F, Santoro I, et al. J Mass Spectrom, 2017, 52(5):290

[10] Zhu J W, Guo S Q, Shu H M, et al. Chinese Agricultural Science Bulletin, 2017, 33(30):43 朱静雯, 郭书巧, 束红梅, 等. 中国农学通报, 2017, 33(30):43

[11] Montoro P, Molfetta I, Maldini M, et al. Food Chem, 2013, 141(2):745

[12] Molina-Calle M, Sanchez de Medina V, Delgado de la Torre M P, et al. Talanta, 2016, 154:263

[13] Fan G Y, Feng F, Zhang F, et al. Chinese Journal of Chromatography, 2018, 36(4):351 范广宇, 冯峰, 张峰, 等. 色谱, 2018, 36(4):351

[14] Molina-Calle M, Priego-Capote F, Luque de Castro M D. Food Chem, 2017, 219:329

[15] Chaturvedula V S, Rhea J, Milanowski D, et al. Phytochem Lett, 2011, 6(2):175