doi:

DOI: 10.3724/SP.J.1123.2018.08002

Chinese Journal of Chromatography (色谱) 2019/37:1 PP.93-99

Determination of glufosinate-ammonium and its three metabolites in litchi and banana by high performance liquid chromatography coupled with triple quadrupole mass spectrometry


Abstract:
A method was developed for the determination of glufosinate-ammonium and its metabolites in litchi and banana by high performance liquid chromatography coupled with triple quadrupole mass spectrometry. The samples were ultrasound extracted by water, centrifuged to precipitate, and filtered through a 0.22 μm Millipore membrane. The sample extract was separated by an Extend-C18 column with 0.1% ammonia-methanol (9:1, v/v) as mobile phase with an isocratic elution, identified by electrospray ionization in negative and multiple reaction monitoring modes, and quantified with external standard method. Moreover, the pretreatment and mass spectrometry conditions were discussed. The method showed good linear relationship in the range of 1-1000 μg/L for glufosinate-ammonium and its metabolites. The average recoveries ranged from 82.9% to 98.6% with the relative standard deviations of 2.6%-6.3% when the litchi and banana samples were spiked at 50, 100, 1000 μg/kg. The limits of detection were 5-10 μg/kg. The method has rapid analysis speed as well as high reliability and sensitivity, which are suitable for the fast determination of glufosinate-ammonium and its metabolites in litchi and banana samples.

Key words:high performance liquid chromatography (HPLC),triple quadrupole mass spectrometry (MS/MS),glufosinate-ammonium,metabolites,litchi,banana

ReleaseDate:2019-01-31 09:03:39



[1] Dai J Z, Chen W G, Qian Q J, et al. Science of Sericulture, 2017, 43(2):268 戴建忠, 陈伟国, 钱秋杰, 等. 蚕业科学, 2017, 43(2):268

[2] Qin G N, Weng L S, Xiao G Y. Journal of Agricultural Biotechnology, 2016, 24(10):1457 秦冠男, 翁绿水, 肖国樱. 农业生物技术学报, 2016, 24(10):1457

[3] Mao Y C, Hung D Z, Wu M L, et al. Clinical Toxicol, 2012, 50:396

[4] Calas A G, Richard O, Meme S, et al. Neuro Toxicology, 2008, 29:740

[5] Schute-Hermann R, Wogan G N, Berry S C, et al. Regul Toxicol Pharmacol, 2006, 44:S1

[6] Zhang Y, Lü D Z, Han B J, et al. Chinese Journal of Pesticide Science, 2014, 16(6):734 张月, 吕岱竹, 韩丙军, 等. 农药学学报, 2014, 16(6):734

[7] Dr. Dugald Malachlan Department of Agriculture, Fisheries and Forestry, Canberra, Australia. Glufosinate ammonium (2012).[2018-06-25]. http://www.fao.org/fileadmin/templates/agphome/documents/Pests_Pesticides/JMPR/Evaluation12/Glufosinate.pdf

[8] Lin Y H, Liu Z C, Yang F, et al. Chinese Journal of Chromatography, 2012, 30(12):1260 林永辉, 刘正才, 杨方, 等. 色谱, 2012, 30(12):1260

[9] Li Y, Sheng Y G, Yi X H, et al. Journal of Instrumental Analysis, 2015, 34(9):993 李优, 盛永刚, 伊雄海, 等. 分析测试学报, 2015, 34(9):993

[10] Wu X G, Chen X Q, Xiao H J, et al. Chinese Journal of Chromatography, 2015, 33(10):1090 吴晓刚, 陈孝权, 肖海军, 等. 色谱, 2015, 33(10):1090

[11] Chen L. Tea Science and Technology, 2014(1):21 陈磊. 茶叶科学技术, 2014(1):21

[12] Zhu L, Chen H P, Zhou S J, et al. Chinese Journal of Analytical Chemistry, 2015, 43(2):271 诸力, 陈红平, 周苏娟, 等. 分析化学, 2015, 43(2):271

[13] Feng Y C, Ma L L, Jia L, et al. Journal of Food Safety and Quality, 2014, 5(4):1147 冯月超, 马立利, 贾丽, 等. 食品安全质量检测学报, 2014, 5(4):1147

[14] Zhang Y, Shu P, Lu M, et al. Journal of Food Safety and Quality, 2017, 8(4):1422 张燕, 舒平, 陆敏, 等. 食品安全质量检测学报, 2017, 8(4):1422

[15] Lin S Y, Huang J L, Li X Y, et al. Journal of Food Safety and Quality, 2016, 7(5):1887 林森煜, 黄嘉乐, 李秀英, 等. 食品安全质量检测学报, 2016, 7(5):1887

[16] Li X J, Meng P J, Wang Y Y, et al. Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2015, 51(3):307 李小娟, 孟品佳, 王燕燕, 等. 理化检验-化学分册, 2015, 51(3):307

[17] Royer A, Beguin S, Tabet J C, et al. Anal Chem, 2000, 72:3826

[18] See H H, Hauser P C, Sanagi M M. Electrophoresis, 2010, 31:575

[19] Chang S Y, Wei M Y. J Chin Chem Soc, 2005, 52:785

[20] Kusters M, Gerhartz M. J Sep Sci, 2010, 33:1139

[21] European Commission. EU Pesticides Database.[2018-06-25]. http://ec.europa.eu/food/plant/pesticides/eu-pesticides-database/public/?event=pesticide.residue.CurrentMRL&language=EN

[22] The Japan Food Chemical Research Foundation. Glufosinate.[2018-06-25]. http://db.ffcr.or.jp/front/pesticide_detail?id=18900

[23] Codex Alimentarius Commission International Food Standards. Glufosinate ammonium.[2018-06-25]. http://www.fao.org/fao-who-codexalimentarius/codex-texts/dbs/pestres/pesticide-detail/en/?p_id=175

[24] GB 2763-2016

[25] The Ministry of Agriculture of the People's Republic of China. Guideline on Pesticide Residue Trials. Beijing:China Agriculture Press, 2004:5 中华人民共和国农业部. 农药残留试验准则. 北京:中国农业出版社, 2004:5