doi:

DOI: 10.3724/SP.J.1123.2018.09025

Chinese Journal of Chromatography (色谱) 2019/37:2 PP.149-154

Determination of coriatin and corianin in plasma and urine using ultra-performance liquid chromatography-triple quadrupole mass spectrometry


Abstract:
An ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) method has been developed for the determination of coriatin and corianin in plasma and urine, which are the biomarkers of poisoning caused by Coriaria sinica Maxim. Plasma and urine samples were extracted and purified using a solid supported liquid/liquid extraction method. Chromatographic separation was performed on a Cortecs C18 column (100 mm×2.1 mm, 1.6 μm) using a gradient elution of methanol and water. Coriatin and corianin were detected using negative electrospray ionization tandem mass spectrometry in multiple reaction monitoring (MRM) mode and quantified via a matrix working standard curve internal standard method; florfenicol was used as the internal standard. The assay was linear in the calibration range of 0.03-5.0 μg/L for coriatin and 0.3-50 μg/L for corianin in plasma, and 0.1-10 μg/L and 1-100 μg/L for coriatin and corianin in urine, respectively. The average recoveries were 86.2%-110% for coriatin and corianin in plasma and urine with relative standard deviations of 5.1%-14.6% (n=6). The limits of detection (S/N=3) for coriatin and corianin were 0.01 μg/L and 0.1 μg/L in plasma, and 0.03 μg/L and 0.3 μg/L in urine, respectively. The method is simple, sensitive and accurate for the determination of coriatin and corianin in plasma and urine for toxicological purposes.

Key words:ultra-performance liquid chromatography-triple quadrupole mass spectrometry,coriatin,corianin,poisoning caused by Coriaria sinica Maxim,plasma,urine

ReleaseDate:2019-02-14 09:31:18



[1] Wang H L. Asia-Pacific Traditional Medicine, 2015, 11(4):92 王会礼. 亚太传统医药, 2015, 11(4):92

[2] Xie L J, Ding M B, Sun C Y. Adverse Drug Reactions Journal, 2008, 10(6):429 谢立璟, 丁茂柏, 孙承业. 药物不良反应杂志, 2008, 10(6):429

[3] Zhao M, Peng J. Guizhou Medical Journal, 2014, 38(12):1109 赵敏, 彭静. 贵州医药, 2014, 38(12):1109

[4] Li Y, Liu W Q. Journal of Pediatric Pharmacy, 2014, 20(6):I 李勇, 刘万琼. 儿科药学杂志, 2014, 20(6):I

[5] Zhou H X. For All Health, 2014, 8(4):398 周慧仙. 大家健康, 2014, 8(4):398

[6] Gong M, transl. Apiculture of China, 1992, 43(4):43 龚蜜, 译. 中国蜂业, 1992, 43(4):43

[7] Xie W W, transl. Apiculture of China, 2012, 63(7):57 谢文闻, 译. 中国蜂业, 2012, 63(7):57

[8] Fields B A, Reeve J, Bartholomaeus A, et al. Food Chem Toxicol, 2014, 72:234

[9] Beasley M, Hood D, Anderson P, et al. N Z Med J, 2018, 131 (1473):59

[10] Kinoshita T, Itaki N, Hikita M, et al. Chem Pharm Bull, 2005, 53(8):1040

[11] Wei H, Zeng F J, Lu M Y, et al. Acta Pharmaceutica Sinica, 1998, 33(9):688 韦宏, 曾凡健, 陆敏仪, 等. 药学学报, 1998, 33(9):688

[12] Kang K, Zhao Y R, Zhou L. World Latest Medicine Information (Electronic Version), 2017, 17(12):104 康凯, 赵月然, 周凌. 世界最新医学信息文摘, 2017, 17(12):104

[13] Wu C Y, Zhang G D. Acta Pharmaceutica Sinica, 1984, 19(1):56 伍朝筼, 章观德. 药学学报, 1984, 19(1):56

[14] Yin Y, Chen H L, Chen L, et al. Chinese Journal of Chromatography, 2015, 33(7):711 殷耀, 陈恵兰, 陈磊, 等. 色谱, 2015, 33(7):711

[15] Zhang X Y, Cai X X. Chinese Journal of Analytical Chemistry, 2011, 39(12):1917 张秀尧, 蔡欣欣. 分析化学, 2011, 39(12):1917

[16] Matuszewski B K, Constanzer M L, Chavez-Eng C M. Anal Chem, 2003, 75 (13):3019