doi:

DOI: 10.3724/SP.J.1123.2018.10042

Chinese Journal of Chromatography (色谱) 2019/37:2 PP.194-200

Effects of different processing methods on water soluble polysaccharide contents and monosaccharide compositions in Cervi Cornu Pantotrichum


Abstract:
The water-soluble polysaccharides in antler velvet were extracted by water extraction and alcohol precipitation. The content was determined by a phenol-sulfuric acid colorimetry. After hydrolysis and derivatization, the various monosaccharide compositions of water-soluble polysaccharides from different parts of the antler velvet after different processing methods were analyzed by ultra high performance liquid chromatography (UPLC). The results showed that the water-soluble polysaccharide contents in wax slices, powder slices, gauze slices and bone slices of the boiled antler velvet were 1.74, 1.67, 1.03 and 1.13 g/kg, respectively, whereas those of the freeze-dried antler velvet were 2.77, 3.07, 1.22 and 3.20 g/kg, respectively. The water-soluble polysaccharide contents in in the same four parts of the antler velvet processed antler velvet without and with blood were 1.55, 1.78, 0.96, 0.77 g/kg, and 1.69, 1.64, 1.01, 1.31 g/kg, respectively. Eight monosaccharides (mannose, glucosamine, ribose, glucuronic acid, galacturonic acid, aminogalactose, glucose and galactose) were detected in the compositions of water-soluble polysaccharides in the antler velvet by using different processing methods. For the same part, the monosaccharide contents in the boiled antler velvet were lower than those in the freeze-dried antler velvet, and the monosaccharide contents (except those of glucosamine and aminogalactose) in the antler velvet processed without blood were lower than those in the antler velvet processed with blood. For different parts processed with the same method, the monosaccharide contents in wax and powder pieces were higher than those in gauze slices and bone pieces. This study provides a theoretical reference for velvet processing and product development.

Key words:ultra high performance liquid chromatography (UPLC),comparative analysis,processing methods,parts,water-soluble polysaccharide,monosaccharide composition,Cervi Cornu Pantotrichum

ReleaseDate:2019-02-14 09:31:23



[1] Pharmacopoeia Commission of People's Republic of China. Pharmacopoeia of People's Republic of China, Part 1. Beijing:China Medical Science and Technology Press, 2015:323 国家药典委员会. 中华人民共和国药典, 一部. 北京:中国医药科技出版社, 2015:323

[2] Kim C T, Gujral N, Ganguly A, et al. Biotechnol Rep, 2014, 4:14

[3] Tseng S H, Sung C H, Chen L G, et al. J Ethnopharmacol, 2014, 151(1):352

[4] Zang Z J, Tang H F, Tuo Y, et al. Asian J Androl, 2016, 18(4):613

[5] Park J, Jeon B, Kang S, et al. Asian Australas J Anim Sci, 2015, 28(9):1303

[6] Xiao X, Xu S Q, Li L, et al. Front Pharmacol, 2017, 8:601

[7] Bi D, Zhang L M L, Tang R W L, et al. Pharm Anal Acta, 2016, 7(4):474

[8] Deng H, Liu Y, Yu J L, et al. China Food Safety Magazine, 2014, 10(23):72 邓浩, 刘影, 于金玲, 等. 食品安全导刊, 2014, 10(23):72

[9] Zhao Y H, Jin X M, Han R. Science and Technology of Food Industry, 2012, 33(12):155 赵玉红, 金秀明, 韩睿. 食品工业科技, 2012, 33(12):155

[10] Chen X G.[MS Dissertation]. Changchun:Jilin University, 2004 陈晓光.[硕士学位论文]. 长春:吉林大学, 2004

[11] Liang H M.[MS Dissertation]. Changchun:Jilin Agriculture University, 2014 梁湖梅.[硕士学位论文]. 长春:吉林农业大学, 2014

[12] Sui Z, Yuan H, Liang Z, et al. Talanta, 2013, 107(2):189

[13] Sui Z, Zhang L, Huo Y, et al. J Pharm Biomed, 2014, 87(1):229

[14] Wang Y H, Sun Y S, Wang Y F, et al. Journal of Instrumental Analysis, 2018, 37(9):995 王燕华, 孙印石, 王玉方, 等. 分析测试学报, 2018, 37(9):995

[15] Sun Y S, Wang Y H, Wang Y F, et al. Chinese Traditional and Herbal Drugs, 2018, 49(4):840 孙印石, 王燕华, 王玉方, 等. 中草药, 2018, 49(4):840

[16] Wang Y H, Jiang Y, Sun Y S, et al. Chinese Journal of Pharmaceutical Analysis, 2018, 38(1):104 王燕华, 姜英, 孙印石, 等. 药物分析杂志, 2018, 38(1):104

[17] Wang Y H, Jin C A, Sun Y S, et al. Chinese Traditional and Herbal Drugs, 2017, 48(12):2431 王燕华, 金春爱, 孙印石, 等. 中草药, 2017, 48(12):2431

[18] Wang Y H, Zhang X L, Zhao H, et al. Chinese Traditional and Herbal Drugs, 2017, 48(15):3085 王燕华, 张秀莲, 赵卉, 等. 中草药, 2017, 48(15):3085

[19] Gong R Z, Wang Y H, Sun Y S, et al. China Journal of Chinese Materia Medica, 2018, 38(3):557 宫瑞泽, 王燕华, 孙印石. 中国中药杂志, 2018, 38(3):557

[20] Li H P, Wang C S. Farming Deer Ecological. Beijing:China Agricultural Press, 2011:407 李和平, 王春生. 生态养鹿. 北京:中国农业出版社, 2011:407

[21] Song J, Zhang Z Q, Li F. Chinese Archives of Traditional Chinese Medicine, 2018, 36(9):2227 宋佳, 张振秋, 李峰. 中华中医药学刊, 2018, 36(9):2227