DOI: 10.3724/SP.J.1123.2019.01049

Chinese Journal of Chromatography (色谱) 2019/37:7 PP.692-700

Synthesis and application of a surface molecularly imprinted adsorbent for di(2-ethylhexyl)phthalate base on graphite oxide

A molecularly imprinted polymer (MIP) base on the surface of graphite oxide (GO) has been developed for the selective recognition of di(2-ethylhexyl)phthalate (DEHP), and applied for the extraction of DEHP in a milk bag sample, with detection by high-performance liquid chromatography. The surface-molecularly imprinted material was prepared by precipitation polymerization in N,N-dimethylformamide (DMF) solvent, using GO as the supporting material, DEHP as the template molecule, and methacrylic acid (MAA) as the functional monomer; the synthesis conditions were also optimized. The obtained GO-MAA-MIP was characterized by Fourier transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM). The adsorption properties of the product were evaluated by adsorption experiments, including adsorption isotherms, kinetics, selectivity, saturated adsorption capacity, adsorption equilibrium time, and reusability. Under optimized conditions, DEHP was effectively extracted in the real sample (milk bag) and detected by HPLC. Linearity was obtained with a correlation coefficient (R2) of 0.9979 in a linear range of 0.5-50 mg/L. The limits of detection and quantitation were 0.03 mg/L and 0.1 mg/L, respectively. The average recoveries of the spiked samples at three concentration levels of DEHP ranged from 81.6% to 92.4% with relative standard deviations (RSDs) less than 7%. The results indicated that the proposed GO-MAA-MIP-SPE (solid phase extraction) protocol with HPLC-UV detection could be applied for the selective analysis of DEHP in real samples.

Key words:surface molecularly imprinted polymer (SMIPs),graphite oxide (GO),solid phase extraction (SPE),di(2-ethylhexyl)phthalate (DEHP),milk plastic bags

ReleaseDate:2019-07-04 09:07:12

[1] Xu B B, Li X M, Zhang Q H, et al. Chinese Journal of Chromatography, 2018, 36(8):786 许兵兵, 李小敏, 张庆合, 等. 色谱, 2018, 36(8):786

[2] Wei J F, Jiang L, Lou C Y, et al. Chinese Journal of Chromatography, 2018, 36(7):678 韦俊芳, 姜磊, 楼超艳, 等. 色谱, 2018, 36(7):678

[3] She Y, Jiang L, Zheng L, et al. Chem Biol Interact, 2017, 265:8

[4] Kalo D, Roth Z. Toxicology, 2017, 377:38

[5] Sun Z A, Qi Y X, Wang X, et al. Chinese Journal of Chromatography, 2018, 36(8):716 孙治安, 祁玉霞, 王霞, 等. 色谱, 2018, 36(8):716

[6] Zheng Y L, Gu L L, Shi J L, et al. Chinese Journal of Chromatography, 2018, 36(7):659 郑亚丽, 顾利莉, 师君丽, 等. 色谱, 2018, 36(7):659

[7] Sun J J, Zhang F F, Liang X M. Chinese Journal of Chromatography, 2018, 36(8):723 孙佳佳, 章飞芳, 梁鑫淼. 色谱, 2018, 36(8):723

[8] Xu W Z, Zhang X M, Huang W H, et al. Chinese Journal of Analytical Chemistry, 2017, 45(4):521 徐婉珍, 张枭明, 黄卫红, 等. 分析化学, 2017, 45(4):521

[9] Li C, Ma X, Zhang X, et al. J Sep Sci, 2017, 40(7):1621

[10] Hu J H, Feng T, Li W L, et al. J Chromatogr A, 2014, 1330:6

[11] He L N, Chen F Y, Tang Y B. Chinese Journal of Environmental Engineering, 2017, 11(6):3532 何莉娜, 陈芳艳, 唐玉斌. 环境工程学报, 2017, 11(6):3532

[12] Du J, Gao R, Mu H. Food Anal Method, 2016, 9(7):1

[13] Ekta R, Santanu P, Ashutosh T, et al. Biosens Bioelectron, 2017, 89:234

[14] Ning F J, Qiu T T, Wang Q, et al. Food Chem, 2017, 221:1797

[15] Fan W Y, He M, You L N, et al. J Chromatogr A, 2016, 1443:1

[16] Hou X Y, Huang W, Tong Y K, et al. Chinese Journal of Chromatography, 2019, 37(1):63 侯星羽, 黄玮, 佟育奎, 等. 色谱, 2019, 37(1):63

[17] Sun Y M, Wu Q, Gao J, et al. Chinese Journal of Chromatography, 2017, 35(3):325 孙亚明, 武琪, 高洁, 等. 色谱, 2017, 35(3):325

[18] Pan S D, Chen X H, Li X P, et al. J Chromatogr A, 2015, 1422:1

[19] Cheng L D, Pan S H, Ding C Y. J Chromatogr A, 2017, 1511:85

[20] Jr W S H, Offeman R E. J Am Chem Soc, 1958, 80(6):1339

[21] GB5009.271-2016

[22] Eveliina R, Jolanta K W, Tonni A K, et al. Chem Eng J, 2010, 161:73

[23] Ho Y S. J Hazard Mater, 2006, 37(48):681