doi:

DOI: 10.3724/SP.J.1123.2019.01029

Chinese Journal of Chromatography (色谱) 2019/37:7 PP.735-741

Determination of streptomycin and dihydrostreptomycin residues in honey by hydrophilic interaction liquid chromatography-tandem mass spectrometry


Abstract:
An analytical method was developed for the determination of streptomycin and dihydrostreptomycin in honey using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The streptomycin and dihydrostreptomycin residues in the test samples were extracted with 20 g/L trichloroacetic acid aqueous solution (including 50 mmol/L phosphate, pH 6.8) and cleaned on an Oasis HLB solid phase extraction column. The products were separated on a SIELC Obelisc R column with gradient elution using 0.5% (v/v) formic acid aqueous solution and acetonitrile as mobile phases. Streptomycin and dihydrostreptomycin were detected by liquid chromatography-tandem mass spectrometry in the positive ion mode using the external standard method. Under the optimal conditions, streptomycin and dihydrostreptomycin showed good linearity (r>0.99) in the range of 2.5-100 μg/L. The LOD and LOQ of the method was 2.0 μg/kg and 5.0 μg/kg, respectively. The spiked recoveries of the analytes from blank honey samples at the three levels of 5.0, 20.0 and 100.0 μg/kg were in the range of 86.9%-113.2% with the relative standard deviations less than 10%. With the advantages of convenience, rapidity, sensitivity and good repeatability, the method is suitable for the detection of streptomycin and dihydrostreptomycin in honey.

Key words:hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS),solid phase extraction (SPE),streptomycin (STR),dihydrostreptomycin (DHSTR),honey

ReleaseDate:2019-07-04 09:07:12



[1] Farouk F, Azzazy H M E, Niessen W M A. Anal Chim Acta, 2015, 890:21

[2] Chen L Y, Zhou Z F, Ma A D. Journal of Hygiene Research, 2010, 39(1):105 陈凌云, 周枝凤, 马安德. 卫生研究, 2010, 39(1):105

[3] He Q, Kong X H, Zhao J, et al. Journal of Instrumental Analysis, 2010, 29(7):691 何强, 孔祥虹, 赵洁, 等. 分析测试学报, 2010, 29(7):691

[4] Wang Z B, Wang X H, Xu B, et al. Journal of Chinese Institute of Food Science and Technology, 2008, 8(5):120 王忠斌, 王向红, 徐蓓, 等. 中国食品学报, 2008, 8(5):120

[5] Wang Z B, Gao Y, Liu Y, et al. Modern Food Science and Technology, 2014, 30(4):260 王志兵, 高杨, 刘洋, 等. 现代食品科技, 2014, 30(4):260

[6] Zhang X Y, Xu J Z, Shen C Y, et al. Chinese Journal of Chromatography, 2008, 26(3):395 张晓燕, 徐锦忠, 沈崇钰, 等. 色谱, 2008, 26(3):395

[7] Chen X H, Liu X L, Dong M S, et al. Food Science, 2004, 25(1):155 陈晓红, 刘小莉, 董明盛, 等. 食品科学, 2004, 25(1):155

[8] Zhang W, Sheng Y G, Gu S Q, et al. Journal of Instrumental Analysis, 2014, 33(6):688 张伟, 盛永刚, 古淑青, 等. 分析测试学报, 2014, 33(6):688

[9] GB/T 22995-2008

[10] SN/T 1925-2007

[11] Wang L, Yang B X, Zhang X S, et al. Chinese Journal of Analytical Chemistry, 2015, 43(5):714 王炼, 杨碧霞, 张新申, 等. 分析化学, 2015, 43(5):714

[12] Arsand J B, Jank L, Martins M T, et al. Talanta, 2016, 154:38

[13] Moreno-González D, Hamed A M, García-Campana A M, et al. Talanta, 2017, 171:74

[14] Huang Y F, Lou X Y, Zhou Z, et al. Chinese Journal of Analytical Chemistry, 2018, 46(3):454 黄原飞, 娄晓祎, 周哲, 等. 分析化学, 2018, 46(3):454

[15] Huang J, Yin Y, Xu J Z, et al. Chinese Journal of Chromatography, 2014, 32(6):566 黄娟, 殷耀, 徐锦忠, 等. 色谱, 2014, 32(6):566

[16] Wang Y, Li S H, Zhang F F, et al. J Chromatogr A, 2016, 1437:8