doi:

DOI: 10.3724/SP.J.1009.2009.00145

Chinese Journal of Natural Medicines (中国天然药物) 2009/7:2 PP.145-149

Bioinformatic Analysis on Jellyfish Hematoxin


Abstract:
AIM: To illustrate the structure and function of five hematoxin sequences reported in four jellyfish and explore the possible mechanism of pathogenesis. METHODS: Bioinformatic methods were used to analyze the composition and sequence of amino acid residues, physico-chemical property, signal peptide, membrane spanning domain, hydrophobicity or hydrophilicity, secondary structure, conserved region and molecular phylogenetic evolution. RESULT and CONCLUSIONS: The results showed that the five jellyfish hematoxins were similar in composition and sequence of amino acid residues and physico-chemical property. The amino acid sequences of jellyfish hematoxins contained membrane spanning domain and hydrophobic regions; with a possible cleavage site in the signal peptide between the amino acid residues 20 and 21; α-helix and random coil were the major motifs of predicted secon-dary structure while β-turn and extended strand spread in the whole protein; There were four conserved amino acid sequences in three of the five hematoxins and similar phylogenetic trees were constructed by both NJ and MP methods.

Key words:Jellyfish,Hematoxin,Bioinformatics

ReleaseDate:2014-07-21 14:37:27

Funds:This project was supported by the Natural Science Foundation of Shanghai Municipal Government (No. 06ZR14111) and “Eleventh Five-Year Plan” for Medical Science Development of PLA (No. 06Z020).



[1]Xiao L, Zhang LM. Research progress on jellyfish hematox-ins [J]. Chin J Mar Drugs, 2007, 26(6): 40-43.

[2]Bloom DA, Burnett JW, Alderslade P. Partial purification of box jellyfish (chironex fleckeri) nematocyst venom isolated at the beachside[J]. Toxicon, 1998, 36(8): 1075-1085.

[3]Nagai H, Takuwa K, Nakao M, et al. Novel pro-teinaceous toxins from the box jellyfish (sea wasp) Carybdea rastoni[J]. Biochem Biophys Res Commun, 2000, 275(2): 582-588.

[4]Yang L, Shang Y. Bioinformatics and modernization of tradi-tional Chinese medicine[J]. Chin Mag Clin Med Prof Res, 2007; 13(13): 1914-1915.

[5]Huang R, Xue C. Bioinformatics [M]. Guangzhou: Sun Yat-sen University, 2003: 123-180.

[6]Zhang SJ. The application of signal peptide sequence in yeast secretory expression system [J]. Med Recapit, 2007, 13(9): 643-645.

[7]Zhai ZH, Wang XZ, Ding MX. Cell biology [M]. Beijing: Higher education press, 2000: 79-240.

[8]Wang TH, Zou XL. Protein theory and technology [M]. Bei-jing: Science press, 2005: 171.

[9]Brinkman D, Burnell J. Identification, cloning and sequenc-ing of two major venom proteins from the box jellyfish, Chironex fleckeri [J]. Toxicon, 2007, 50(6): 850-860.

PDF