DOI: 10.3724/SP.J.1005.2008.00185

Hereditas (Beijing) (遗传) 2008/30:2 PP.185-189

Expression of FoxO1 mRNA in muscle tissue of Bamei,Landrace and Landrace × Bamei

Total RNA was extracted from soleus muscle, gastrocnemius muscle and extensor digitorum longus in hind leg of the 6-month-old male Bamei, Landrace, and the crossbred pigs (Landrace×Bamei). The primers of pig FoxO1 were designed and synthesized according to its homologous sequences of human, chimpanzee and rat. The optimal reaction condition and system of PCR was sieved to clone pig FoxO1gene. Subsequently, the differential expression of FoxO1 mRNA was investigated in different types of skeletal muscle from Bamei, Landrace, and the crossbred pigs by SQ RT-PCR. The expression level of β-actin was determined as a loading control. The results showed that the expression level of FoxO1 was different in various economic pig breeds and skeletal muscle types. The expression of FoxO1 inskeletalmuscleofBamei and the crossbred pigs was significantly higher than that of Landrace (P<0.01). Moreover, the expression of FoxO1was lowest in soleus muscle, which mainly contains typeⅠmuscle fiber, and most abundant in extensor digitorum longus, which mainly contains typeⅡb muscle fiber. This suggests that FoxO1 expression level has the negative correlation with the content of typeⅠmuscle fiber. Skeletal muscle development of different economic types of pig breeds may be closely related to the regulation of FoxO1gene.

Key words:FoxO1gene,muscle tissue,fiber-type,pig

ReleaseDate:2014-07-21 14:08:24

Funds:Supported by Key Project of Chinese National Programs for Fundamental Research and Development (973 Program) (No. 2004CB117506)

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