Hereditas (Beijing) (遗传) 2008/30:4 PP.463-468
PCR-SSCP was applied to analyze the polymorphisms ofCXCR2gene and its relationships with milk quality and mastitis in 160 cattle samples including Holstein dairy cow, Simmental dairy cow and Tongjiang cattle. The results showed that there were 3 SNP loci in CXCR2 gene, which located at 684 bp, 777 bp, 861 bp, respectively. Five alleles (A, B, C, EandF) were determined based on the 3 SNP loci. The genotypes for each locus wereBCandCC, AA and AB, CC andBCat 685 bp, 777 bp and 861 bp, respectively. Results also demonstrated that genotypesBC, CCandFFwere probably relevant with mastitis, butAA, BBandEEmay resist the mastitis of cattle. The genotypesAA, AB andEEmay have better milk quality (extremely significant difference or significant difference) than other genotypes.
Funds：Supported by National 115 Key Technology R＆D Program (No.2006BAD04A17)
Knall C, Young S, Nick JA, Buhl AM, Worthen GS, Johnson GL. Interleukin-8 regulation of the Ras/Raf/mitogen- activated protein kinase pathway in human neutrophils.J Biol Chem,1996,271(5):2832-2838.
Loetscher P, Seitz M, Clark-Lewis I, Baggiolini M, Moser B. Both interleukin-8 receptors independently mediate chemotaxis. Jurkat cells transfected with IL-8R1 or IL-8R2 migrate in response to IL-8, GRO alpha and NAP-2.FEBS Lett,1994,341(2-3):187-192.
Paape M, Mehrzad J, Zhao X, Detilleux J, Burvenich C. Defense of the bovine mammary gland by polymorphonuclear neutrophil leukocytes.J Mammary Gland Biol Neoplasia,2002,7(2):109-121.
LI Jian-Bin, SUN Shao-Hua, TIAN Yu-Ze. Study on variability of somatic cell counts in the milk of Chinese Holstein cattle. Acta Ecologae Animalis Domastici,2006,27(3):78-81.李建斌, 孙少华, 田雨泽. 中国荷斯坦牛乳中体细胞数变化规律的研究. 家畜生态学, 2006, 27(3): 78-81.
Damaj BB, McColl SR, Neote K, Songqing N, Ogborn KT, Hebert CA, Naccache PH. Identification of G-protein binding sites of the human interleukin-8 receptors by functional mapping of the intracellular loops.FASEB J,1996,10(12):1426-1434.
Chrystal MA, Seykora AJ, Hansen LB, Freeman AE, Kelley DH, Healey MH. Heritability of teat-end shape and the relationship of teat-end shape with somatic cell score for an experimental herd of cows.J Dairy Sei,2001,84(11):2549-2554.
Ashwell MS, Rexroad CE Jr, Miller RH, VanRaden PM. Mapping economic trait loci for somatic cell score in Holstein cattle using microsatellite markers and selective genotyping.Anim Genet,1996,27(4):235-242.
Vilkki HJ, Dekoning DI, Elo K, Velmala R. Multiple marker mapping of quantitative trait loci of Finnish diary cattle by regression.J Dairy Sci,1997,80(1):198-204.
Yao J, Burton JL, Saama P, Sipkovsky S, Coussens PM. Generation of EST and cDNA microarray resources for the study of bovine immunobiology.Acta Vet Scand,2001,42(3):391-405.
Burton JL, Madsen SA, Yao J, Sipkovsky SS, Coussens PM. An immunogenomics approaches to understanding periparturient mastitis susceptibility in dairy cows.Acta Vet Scand,2001,42(3):407-424.
Madsen SA, Weber PS, Burton JL. Altered expression of cellular genes in neutrophils of periparturient dairy cows.Vet Immunol Irnmunopathol,2002,86(3-4):159-175.
Coussens PM, Nobis W. Bioinformatics and high throughput approach to create genomic resources for the study of bovine immunobiology.Vet Immunol Immunopathol,2002,86(3-4):229-244.
YE Su-Cheng, CHU Ming-Xing, CHEN Guo-Hong. Progress on MHC polymorphism and its relationship with economic traits in dairy cattle.Hereditas(Beijing),2003,25(1):89-92.叶素成, 储明星, 陈国宏. 奶牛MHC基因多态性及其与经济性状关系的研究进展. 遗传, 2003, 25(1): 89-92.
WANG Xing-Ping, XU Shang-Zhong, MA Teng-He, GAO Xue, REN Hong-Yan, CHEN Jin-Bao. Genetic variation in the 5′ flanking region of bovine TLR4 gene and correlation with mastitis.Hereditas(Beijing),2006,28(12):1520-1524.王兴平, 许尚忠, 马腾壑, 高雪, 任红艳, 陈金宝. 牛TLR4基因5′侧翼区的遗传变异与乳房炎的关联. 遗传, 2006, 28(12): 1520-1524.
WANG Xing-Ping, XU Shang-Zhong, GAO Xue, REN Hong-Yan, CHEN Jin-Bao. Genetic polymorphism of TLR4 gene and correlation with mastitis in cattle.Journal of Genetics and Genomics,2007,34(5):406-412.王兴平, 许尚忠, 高雪, 任红艳, 陈金宝. 奶牛TLR4基因的多态性与乳房炎的相关性研究. 遗传学报, 2007, 34(5): 406-412.
TANG Shu-Zhen, LI Yong-Peng. Use SCC to monitor mammitis.China Dairy Cattle,1999,4:45-46.唐淑珍, 李永鹏. 利用体细胞计数监控乳房炎. 中国奶牛, 1999, 4: 45-46.
Youngerman SM, Saxton AM, Oliver SP, Pighetti GM. Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle,J Dairy Sci,2004,87(8):2442-2448.
Youngerman SM, Saxton AM, Pighetti GM. Novel single nucleotide polymorphisms and haplotypes within the bovine CXCR2 gene.Immunogenetics,2004,56(5):355-359.