doi:

DOI: 10.3724/SP.J.1005.2009.00407

Hereditas (Beijing) (遗传) 2009/31:4 PP.407-411

Studies on quantitative trait loci related to activity of lactate dehydrogenase in common carp (Cyprinus carpio)


Abstract:
Microsatellite markers were used to make marker regression analysis on activity of lactate dehydrogenase based on double pseudo-testcross strategy using Windows Map Manager2.0 software. The parents that came from the cross between progenies of Hebao-cold tolerance red carp and Barbless carp and F2 progenies were used as segregating populations. For maker regression, a total of 12 markers associated with activity of lactate dehydrogenase were significant at P<0.05 and HLJE222 was significant at P<0.01. The variance explained by these loci, ranged from 4.00% to 10.00%. Locus HLJE222 was closely linked to the gene related to activity of lactate dehydrogenase of common carp. For further identification, EST-SSR markers were used to screen the protein and nucleotide database using bioinformatics tools in order to find the homologies. High sequence similarities of HLJE222 marker were observed with the nucleotide sequence of DAZ associated protein 1mRNA of zebrafish(94%), and protein sequence of DAZ associ-ated protein 1(97%). DAZ protein is one of the short chain dehydrogenases, which is an important enzyme in the process of glucose metabolism in the organisms. This family contains a wide variety of dehydrogenases. This indicates that locus HLJE222 was closely linked to the gene associated with activity of lactate dehydrogenase of common carp.

Key words:common carp(Cyprinus carpio),marker regression,lactate dehydrogenase,double pseudo-testcross strategy,EST-SSR

ReleaseDate:2014-07-21 14:39:20



[1] 徐云碧, 朱立煌. 分子数量遗传学. 北京: 中国农业出版社, 1994.

[2] 陈思种, 陈芳. 关于乳酸脱氢酶的学习和总结. 卫生职业教育, 2008(2): 71-72.

[3] Tanck MWT, Palstra AP, van de Weerd, van de Weerd M, Leffering CP, van der Poel JJ, Bovenhuis H, Komen J. Segregation of microsatellite alleles and residual het-erozygosity at single loci in homozygous androgenetic common carp (Cyprinus carpio L.).Genome, 2001, 44(5): 743-751.

[4] Sun X, Liang L. A genetic linkage map of common carp (Cyprinus earpio L.) and mapping of a locus associated with cold tolerance.Aquaculture, 2004, 238(1): 165-172.

[5] 张研, 梁利群, 常玉梅, 侯宁, 鲁翠云, 孙效文. 鲤鱼体长性状的QTL定位及其遗传效应分析. 遗传, 2007, 29(10): 1243-1248.

[6] 岳志芹, 王伟继, 孔杰, 戴继勋, 王清印. AFLP 分子标记构建中国对虾遗传连锁图谱的初步研究. 高技术通讯, 2004, 5: 88-93.

[7] 刘贤德, 刘晓, 张国范. 皱纹盘鲍遗传图谱构建及生长相关性状的QTL定位[学位论文]. 中科院海洋研究所, 2005.

[8] Zhang Y, Liang LQ, Jiang P, Li DY, Lu CY, Sun XW. Genome evolution of common carp (Cyprinus carpio) as revealed by the analysis of microsatellite loci in a gynogentic family.J Genet Genomics,2008,35(2):97-103.

[9] David L, Jinggui F, Palanisamy R, Hillel J, Lavi U. Polymorphism in ornamental and common carp strains (Cyprinus carpio L.) as revealed by AFLP analysis and a new set of microsatellite markers.Mol Gen Genomics,2001,266(3):353-362.

[10] 李桂源, 钱骏. 基于www的生物信息学应用指南. 长沙: 中南大学出版社, 2004.

[11] 宋宇轩, 曹斌云. 表达序列标签(ESTs)及其应用. 家畜生态, 25(4): 152-155.

[12] 钱骏, 董利, 李桂源. 表达序列标签数据库搜索鉴定小鼠UBAP1基因及其数字化表达分析. 生物化学与生物物理进展, 2002, 29(2): 323-327.

[13] 徐鹏, 王慧, 李群, 盖钧镒, 喻德跃. 大豆油分含量QTL的定位. 遗传, 2007, 29(1): 92-96.

[14] 侯宁, 张研, 鲁翠云, 李勇, 李大宇, 季旭, 丁雷, 孙效文. 微卫星DNA标记分析德国镜鲤的遗传潜力. 遗传, 2007, 29(12): 1509-1518.

[15] Maliepaard C, Jansen J, van Ooijen JW. Linkage analysis in a full-sib family of an outbreeding plant species: over-view and consequences for applications.Genet Res,1997,70(3): 237-250.

PDF