DOI: 10.3724/SP.J.1005.2008.00907

Hereditas (Beijing) (遗传) 2008/30:7 PP.907-912

Expression of ER, bcl-2, and p53 mRNA in early hybrid embryos of chicken-quail

Using artificial insemination, 100 female quails were crossed with 10 male chickens. The eggs were collected and hatched in the same incubator. The sex of live hybrid embryos from 66 to 120 hatch hours was determined using multiply PCR of Wpkci. Total 300 male and female embryos at various hatch times were sampled and the relative mRNA abundance of ER, bcl-2, and p53 in the embryos was detected by RT-PCR using b -actin as the internal standard. The effects of ER, bcl-2, and p53 on the early embryonic development for hybrids between chicken and quail were analyzed. The results showed ER mRNA expression of female hybrids were higher than male hybrids from 66 to 84 hatch hours with a highly significant difference (P<0.01), which indicated that the sex differentiation of hybrids was perhaps happened between 66 to 84 h of embryo stage. The obvious sequential expression of bcl-2 and p53 in the embryonic development indicated that the bcl-2 and p53 genes had an important effect on the development of the hybrid embryos.

Key words:chicken,quail,early hybrid embryo,ER,bcl-2,p53,mRNA expression

ReleaseDate:2014-07-21 14:10:17

Funds:Supported by National Natural Science Foundation of China (No. 30360071; 30660125)

[1] YU Chun-Mei, ZHAO Zong-Sheng, LI Da-Quan, LI Yan. Early growth performance measurement for chicken(♂) quail (♀) and their hybrids. Journal of Shihezi University (Natural Science), 2003, 7(1): 25-28.余春梅, 赵宗胜, 李大全, 李岩. 鸡与鹌鹑及其属间杂交种早期生长性能的测定. 石河子大学学报(自然科学版), 2003, 7(1): 25-28.

[2] Yoshihiro T. The sex ratio of Chicken-Quail hybrids. Japanese Poultry Science, 1982, 19 (1): 53-54.

[3] TANG Sen-Sen, HU Xuan, LIAO He-Rong, ZHAO Zong-Sheng, LI Da-Quan. Application of mRNA differential display to screen different display gene expression during early embryonic development of chicken and quail hybridization. Journal of Shihezi University (Natural Science), 2006, 24(4): 450-453.唐森森, 胡煊, 廖和荣, 赵宗胜, 李大全. 鸡与鹌鹑杂交种早期胚胎发育特异表达基因的初步筛选. 石河子大学学报(自然科学版), 2006, 24(4): 450-453.

[4] YUAN Yuan, ZHU Zuo-Yan, LAI Zhi-Chun, ZHANG Wen-Xia. Progress on breast cancer metastasis suppressor 1 (BRMS1). Hereditas(Beijing), 2006, 28(9): 1141-1148.袁圆, 朱作言, 赖志春, 张文霞. 乳腺癌转移抑制因子BRMS1研究进展. 遗传, 2006, 28(9): 1141-1148.

[5] Andrews JE, Smith CA, Sinclair AH. Sites of estrogen receptor and aromatase expression in the chicken embryo. Gen Comp Endocrinol, 1997, 108: 182-190.

[6] Smith CA, Andrews JE, Sinclair AH.Gonadal sex differentiation in chicken embryos:expression of estrogen receptor and aromatase genes. Steroid Biochem Mol Biol, 1997, 60 (5-6): 295-302.

[7] Hockenbery D, Nu?ez G, Miliman C, Schreiber RD, Korsmeyer SJ. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature, 1990, 348: 334-346.

[8] Hofseth LJ, Hussain SP, Harris CC. p53: 25 years after its discovery. Trends Pharmacol Science, 2004, 25(4): 177-181.

[9] Atug F, Turkeri L, Ozyurek M, Akdas A. Bcl-2 and p53 overexpression as associated risk factors in transitional cell carcinoma of the bladder. Int Urol Nephrol, 1998, 30: 455-461.

[10] CHEN Zuo-Zhou. Selective mechanisms and the DNA injury hypothesis of cell senescence. Hereditas(Beijing), 2001, 23 (6): 559-563.陈作舟. 筛选机制与细胞衰老的DNA 损伤假说. 遗传, 2001, 23 (6): 559-563.

[11] Eguchi Y, Ewert DL, Tsujimoto Y. Isolation and characterization of chicken bcl-2 gene: expression in a variety of

[12] Soussi T,Begue A,Kress M.Nucleotide sequence of a DNA encoding the chicken p53 nudear oncoprotein.Nucleic Acids Res,1988,16(23):11383.

[13] Derry WB, Putzke AP, Rothman JH. Caenorhabditis elegan p53: role in apoptosis, meiosis, and stress resistance. Science, 2001, 294: 591-595.

[14] Hori T, Asakawa S, Itoh Y, Shimizu N, Mizuno S. Wpkci, encoding an altered form of PKCI, is conserved widely on the avian Wchromosome and expressed in early female embryos:implication of its role in female sex determination. Mol Biol Cell, 2000, 11(10): 3645-3660.

[15] Nakabayashi O, Kikuchi H, Kikuchi T, Mizuno S. Differential expression of genes for aromatase and estrogen receptor during the gonadal development in chicken embryos. J Mol Endocrinol, 1998, 20: 193-202.

[16] MI Yu-Ling, ZHANG Cai-Qiao. Molecular mechanisms of avian gonadal devlopment and sex control. Joumal of Agricultural Biotechnology, 2004, 12(5): 602-605.米玉玲, 张才乔. 禽类性腺发育和性别调控的分子机理. 农业生物技术学报, 2004, 12(5): 602-605.

[17] XIE Mei-Na, ZHANG Cai-Qiao, MI Yu-Ling, ZENG Wei-Dong. Effects of follicle stimulating hormone and estrogen on development of embryonic chicken germ cell in vitro. Zoological Research, 2004, 25(1): 53-56.解美娜, 张才乔, 米玉玲, 曾卫东. 卵泡刺激素和雌激素对培养的鸡胚卵巢生殖细胞增殖的影响. 动物学研究, 2004, 25( 1): 53-56.

[18] LIU Chun-Ling, DING Jia-Tong, WANG Jian-Wu, CHENG Wen-Jia. Progress of study on ER. Animal Science and Veterinary Medicine, 2003, 20 (8): 35-36.刘春玲, 丁家桐, 王建武, 程文佳. 雌激素受体的研究进展.动物科学与动物医学, 2003, 20 (8): 35-36.

[19] Lichnovsky V, Kolár Z, Murray P, Hlobilková A, Cernochová D, Pospísilová E, Vojtesek B, Nenutil R. Differences in p53 and bcl-2 expression in relation to cell proliferation during the development of human embryos. Mol Pathol, 1998, 51: 131-137.

[20] Miyashita T, Krajewski S, Krajewska M, Wang HG, Lin HK, Liebermann DA, Hoffman B, Reed JC. Tumor supressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo. Oncogene, 1994, 9: 1799-1805.

[21] Yu CL,Driggers P,Barrera-Hernandez G,Nunez SB,Segars JH,Cheng SY.The tumor suppressor P53 is a negative regulator of estrogen receptor signaling pathways.Biochemical and Biophysical.Research Communicattions,1997,(239):617-620.

[22] Belcredito S,Vegeto E,Brusadelli A,Ghisletti S,Nussi P,Ciand P,Maggi A.Estrogen neuroprotection:the involvement of the Bcl-2 binding protein BNIP2.Brain Research Reviews,2001,(37):335-342.