DOI: 10.3724/SP.J.1231.2013.38803

Journal of Fisheries of China (水产学报) 2013/37:12 PP.1795-1800

Changes in blood moisture,osmolality and ion concentration of juvenile Chinese sturgeon(Acipenser sinensis)in the Yangtze Estuary

To understand salinity adaptation and regulatory status of juvenile Chinese sturgeon in the Yangtze Estuary,the 7-month-old juveniles were transferred directly from freshwater(0)to salinity 5,10,and 15 for 32 days.And the blood moisture content,serum osmolality and ion concentrations of fish exposed to various salinities were measured respectively at 0.5,1,2,4,8,16,and 32 days after transfer.Results showed that:all measured blood parameters of juveniles in freshwater control and salinity 5 kept the same pattern,and those are not significantly different.In the salinity 10 and 15 groups,blood moisture content of juveniles decreased first,and then increased.The decrements were positively correlated to salinities,and the blood moisture content of juveniles in all groups was not significantly different at 16 days after transfer.Changes in serum osmolality and Na+,Cl- concentrations of juveniles exposed to salinity 10 and 15 were consistent,i.e.increased significantly,then decreased and reached a new steady state.However,change in concentration of serum K+ in juveniles exposed to salinity 10 and 15 differed from the serum osmolality and Na+,Cl- concentrations.It decreased and reached a new stable state.The results indicated that salinity acclimation process of juvenile Chinese sturgeon,like other euryhaline fishes,can be divided into two periods:the critical period and the regulatory period.

Key words:juvenile Acipenser sinensis,salinity,osmoregulation,ion concentration,the Yangtze Estuary

ReleaseDate:2015-04-19 11:27:12

[1] Potts W T W,Rudy P P.Aspects of osmotic and ionic regulation in the sturgeon[J].The Journal of Experimental Biology,1972,56(3):703-715.

[2] Evans D H,Claiborne J B.The physiology of fishes[M].3rd ed.Boca Raton:CRC Press,2005:177-230.

[3] Gershanovich A D,Smith T I J.International symposium on sturgeons proceedings[C].Moscow:VNIRO,1995:43-61.

[4] 庄平,刘健,王云龙,等.长江口中华鲟自然保护区科学考察与综合管理[M].北京:海洋出版社,2009:239-240.

[5] Wei Q,Ke F,Zhang J,et al.Biology,fisheries,and conservation of sturgeons and paddlefish in China[J].Environmental Biology of Fishes,1997,48(1-4):241-245.

[6] He X,Zhuang P,Zhang L,et al.Osmoregulation in juvenile Chinese sturgeon(Acipenser sinensis Gray)during brackish water adaptation[J].Fish Physiology and Biochemistry,2009,35(2):223-230.

[7] Zhao F,Qu L,Zhuang P,et al.Salinity tolerance as well as osmotic and ionic regulation in juvenile Chinese sturgeon(Acipenser sinensis Gray,1835)exposed to different salinities[J].Journal of Applied Ichthyology,2011,27(2):231-234.

[8] 庄平,章龙珍,李长松,等.鱼类电麻醉方法:中国,200410093180.9[P].2007-11-21.

[9] 赵峰,庄平,章龙珍,等.盐度驯化对史氏鲟鳃Na+/K+-ATP酶活力、血清渗透压及离子浓度的影响[J].水产学报,2006,30(4):444-449.

[10] 赵峰,庄平,李大鹏,等.盐度对施氏鲟和西伯利亚鲟稚鱼的急性毒性[J].生态学杂志,2008,27(6):929-932.

[11] Nonnote G,Truchot J P.Time course of extracellular acid-base adjustments under hypo- and hyperosmotic condition in the euryhaline fish Platichthys flesus[J].Journal of Fish Biology,1990,36(2):181-190.

[12] Gilles B,Patrick P.How should salinity influence fish growth[J].Comparative Biochemistry and Physiology,2001,130C(4):411-423.

[13] Natochin Y V,Lukianenko V I,Kirsanov V I,et al.Features of osmotic and ionic regulation in Russian sturgeon(Acipenser guldenstadtii Brand)[J].Comparative Biochemistry and Physiology,1985,80A(3):297-302.

[14] 屈亮,庄平,章龙珍,等.盐度对俄罗斯鲟幼鱼血清渗透压、离子含量及鳃丝Na+/K+-ATP酶活力的影响[J].中国水产科学,2010,17(2):243-251.

[15] McEnroe M,Cech J J.Osmoregulation in juvenile and adult white sturgeon,Acipenser transomntanus[J].Environmental Biology of Fishes,1985,14(1):23-30.

[16] Rodríguez A,Gallardo M A,Gisbert E,et al.Osmoregulation in juvenile Siberian sturgeon(Acipenser baerii)[J].Fish Physiology and Biochemistry,2002,26(4):345-354.

[17] LeBreton G T O,Beamish F W H.The influence of salinity on ionic concentrations and osmolarity of blood serum in lake sturgeon,Acipenser fulvescens[J].Environmental Biology of Fishes,1998,52(4):477-482.

[18] Birstein V J,Bemis W E,Waldman J R.The threatened status of Acipenseriform species:a summary[J].Environmental Biology of Fishes,1997,48(1-4):427-435.

[19] Gilliam J F,Fraser D F.Habitat selection under predation hazard:test of a model with foraging minnows[J].Ecology,1987,68(6):1856-1862.

[20] Stillman R A,Goss-Custard J D,West A D,et al.Predicting mortality in novel environments:tests and sensitivity of a behavior-based model[J].Journal of Applied Ecology,2000,37(4):564-588.