DOI: 10.3724/SP.J.1118.2013.01234

Journal of Fishery Sciences of China (中国水产科学) 2013/20:6 PP.1234-1246

Changes in the characteristics of the bacterial community structure during habitat restoration of aquaculture sites in Xiangshan Bay

Xianshan Bay (121°25′–122° 03′E, 29°05′–29°46′N) is located in the middle of Zhejiang province along the coast, a long and narrow semi-closed bay with the water area of 1 445 km2, and average water depth of 20 m. In recent years, the scale of aquaculture became larger and larger, and with pollution from land-based source, which made the environment in this bay increasingly deteriorate. During 2009–2011, we expanded the scale of macro-algae culture near the cage area of middle Xiangshan Bay to try to restore the habitat around the areas of cage culture. To assess the response, we measured the characteristics of the bacterial communities in the sediment of cage and algae culture areas using PCR, denaturing gradient gel electrophoresis (DGGE), cloning, and sequencing. The bacterial communities in the sediment environment of the cage culture area consisted primarily of 7 phyla lineages of the domain bacteria. There was an increase in bacterial diversity during restoration and the dominant bacterial group changed from Acinetobacter sp., Aminobacter aminovorans strain EE9, and Pseudomonas sp. to the uncultured actinobacterium clone HAHS13.051, Haliea sp. DSW4-37, and Ochrobactrum sp.. The bacterial community in the algal culture area consisted primarily of 5 phyla lineages of the domain bacteria and the structure remained relatively stable. RDA analysis revealed that ammonia and COD are the primary factors influencing the variation in bacterial community structure. The improving trend in bacterial communities was consistent with improvements in chemical indices. Our results demonstrate that the use of macroalgae to restore the habitat of cage culture areas was effective, and suggest that bacterial community structure can be used as an index to evaluate the effectiveness of habitat restoration.

Key words:Xiangshan Bay,cage culture area,algae culture area,habitat restoration,bacterial community,PCR-DGGE

ReleaseDate:2014-07-21 16:58:33