doi:

DOI: 10.3724/SP.J.1118.2019.18264

Journal of Fishery Sciences of China (中国水产科学) 2019/26:3 PP.473-483

Effects of high levels of niacin on lipid metabolism and body fat deposition in genetically improved farmed tilapia, Oreochromis niloticus


Abstract:
To investigate the effects of high niacin levels on lipid metabolism and body fat deposition in fish, two experiments on genetically improved farmed tilapia (GIFT, Oreochromis niloticus) were designed. In experiment I, to determine whether high niacin can decrease serum lipid content in fish and its appropriate level, a model of hyperlipidemia was established by first feeding GIFT (initial body weight:15.28±0.23 g) a high-carbohydrate and high-fat diet for 40 d; subsequently, these fish were fed diets containing 500 mg/kg, 1000 mg/kg, and 2000 mg/kg niacin. The results showed that the addition of 500-2000 mg/kg niacin in the diet could reduce fish serum cholesterol and triglyceride levels, and that 1000-2000 mg/kg niacin had more highly significant effects. In experimental Ⅱ, a 2×2 factorial study was conducted to investigate the effects of two diet types (the control diet and high-carbohydrate and high-fat diet) and two niacin levels (100 mg/kg and 1000 mg/kg) on the growth, feed utilization, serum lipid level, and body composition of GIFT (initial body weight:24.45±0.07 g). The experiment lasted for 8 weeks. The results showed that neither of the diet types nor niacin levels had significant effects (P > 0.05) on the final mean weight, weight gain, specific growth rate, feed conversion ratio, feeding intake, condition factor, viscerosomatic index, mesenteric fat index, or the apparent digestibility of crude protein and crude fat, or crude fat, crude protein, moisture, and ash content of the whole fish. Apparent digestibility of dry matter and energy, and the levels of serum cholesterol, triglycerides and free fatty acid (FFA), and the crude fat contents of liver, viscera, and abdominal muscle of fish fed the high-carbohydrate and high-fat diet were significantly higher (P < 0.05) than those of the control group. The serum low density lipoprotein, cholesterol and FFA, and liver crude fat contents of fish fed the diets contain 1000 mg/kg niacin were significantly lower (P < 0.05) than those fed the diets containing 100 mg/kg niacin. There were significant interactions (P < 0.05) between dietary types and niacin levels on serum high density lipoprotein and cholesterol contents. These results indicated that the supplementation of 1000 mg/kg niacin in the diet would not affect the growth performance and feed utilization of GIFT. However, it could reduce the levels of serum lipids and liver fat content.

Key words:tilapia; niacin; lipid metabolism; body fat deposition

ReleaseDate:2019-07-04 08:50:30



[1] Hansen A C, Waagbø R, Hemre G I. New B vitamin recommendations in fish when fed plant-based diets[J]. Aquaculture Nutrition, 2015, 21(5):507-527.

[2] Foster J W, Park Y K, Penfound T, et al. Regulation of NAD metabolism in Salmonella typhimurium:molecular sequence analysis of the bifunctional nadR regulator and the nadA-pnuC operon[J]. Journal of Bacteriology, 1990, 172(8):4187-4196.

[3] Mani P, Rohatgi A. Niacin therapy, HDL cholesterol, and cardiovascular disease:is the HDL hypothesis defunct?[J]. Current Atherosclerosis Reports, 2015, 17:43.

[4] Ganji S H, Tavintharan S, Zhu D M, et al. Niacin noncompetitively inhibits DGAT2 but not DGAT1 activity in HepG2 cells[J]. Journal of Lipid Research, 2004, 45(10):1835-1845.

[5] Ganji S H, Kashyap M L, Kamanna V S. Niacin inhibits fat accumulation and oxidative stress in human hepatocytes and regresses hepatic steatosis in experimental rat model[J]. Journal of Clinical Lipidology, 2014, 8(3):349-350.

[6] Liu L, Li C, Fu C, et al. Dietary niacin supplementation suppressed hepatic lipid accumulation in rabbits[J]. Asian-Australasian Journal of Animal Sciences, 2016, 29(12):1748-1755.

[7] NRC. Nutrient Requirements of Fish and Shrimp[M]. Washington, DC:National Academy Press, 2011:192.

[8] Mohamed J S, Ibrahim A. Quantifying the dietary niacin requirement of the Indian catfish, Heteropneustes fossilis (Bloch), fingerlings[J]. Aquaculture Research, 2001, 32(3):157-162.

[9] Ahmed I. Effect of dietary niacin on growth and body composition of two Indian major carps rohu, Labeo rohita, and mrigal, Cirrhinus mrigala (Hamilton), fingerlings based on dose-response study[J]. Aquaculture International, 2011, 19(3):567-584.

[10] Xiang Y, Zhou X Q, Feng L, et al. Dietary niacin requirement of juvenile jian carp (Cyprinus carpio var. Jian)[J]. Chinese Journal of Animal Nutrition, 2008, 20(5):527-533.[向阳, 周小秋, 冯琳, 等. 幼建鲤的烟酸需要量[J]. 动物营养学报, 2008, 20(5):527-533.]

[11] Wu F, Jiang M, Zhao Z Y, et al. The dietary niacin requirement of juvenile Ctenopharyngodon idellus[J]. Journal of Fisheries of China, 2008, 32(1):65-70.[吴凡, 蒋明, 赵智勇, 等. 草鱼幼鱼对烟酸的需要量[J]. 水产学报, 2008, 32(1):65-70.]

[12] Huang F, Wen H, Wu F, et al. The dietary niacin requirement of large GIFT tilapia[J]. Journal of South China Agricultural University, 2013, 34(2):235-240.[黄凤, 文华, 吴凡, 等. 吉富罗非鱼成鱼对烟酸的需要量[J]. 华南农业大学学报, 2013, 34(2):235-240.]

[13] Jiang M, Huang F, Wen H, et al. Dietary niacin requirement of GIFT tilapia, Oreochromis niloticus, reared in freshwater[J]. Journal of the World Aquaculture Society, 2014, 45(3):333-341.

[14] Du Z Y. Causes of fatty liver in farmed fish:a review and new perspectives[J]. Journal of Fisheries of China, 2014, 38(9):1628-1638.[杜震宇. 养殖鱼类脂肪肝成因及相关思考[J]. 水产学报, 2014, 38(9):1628-1638.]

[15] Shiau S Y, Suen G S. Estimation of the niacin requirements for tilapia fed diets containing glucose or dextrin[J]. Journal of Nutrition, 1992, 122(10):2030-2036.

[16] Shiau S Y, Yu Y P. Dietary supplementation of chitin and chitosan depresses growth in tilapia, Oreochromis niloticus×O. aureus[J]. Aquaculture, 1999, 179(1-4):439-446.

[17] Shanghai Medical Laboratory. Clinical Biochemistry Test (volume 1)[M]. Shanghai:Shanghai Scientific & Technical Publishers, 1979:168-170.[上海市医学化验所. 临床生化检验(上册)[M]. 上海:上海科学技术出版社, 1979:168-170.]

[18] Yu H L, Bi Y X, Ma W W, et al. Long-term effects of high lipid and high energy diet on serum lipid, brain fatty acid composition, and memory and learning ability in mice[J]. International Journal of Developmental Neuroscience, 2010, 28(3):271-276.

[19] Grundy S M, Mok H Y, Zech L, et al. Influence of nicotinic acid on metabolism of cholesterol and triglycerides in man[J]. Journal of Lipid Research, 1981, 22(1):24-36.

[20] Lukasova M, Hanson J, Tunaru S, et al. Nicotinic acid (niacin):new lipid-independent mechanisms of action and therapeutic potentials[J]. Trends in Pharmacological Sciences, 2011, 32(12):700-707.

[21] Akpan E, Etim O, Bassey U, et al. The effects of nicotinic acid on lipid profile of albino wistar rats[J]. Journal of Physiology and Pharmacology Advances, 2014, 4(8):401-406.

[22] Le Bloc'h J, Leray V, Nazih H, et al. Nicotinic acid accelerates HDL cholesteryl ester turnover in obese insulin-resistant dogs[J]. PLoS ONE, 2015, 10(9):e0136934.

[23] Navarre O, Halver J E. Disease resistance and humoral antibody production in rainbow trout fed high levels of vitamin C[J]. Aquaculture, 1989, 79(1-4):207-221.

[24] Ortu O J, Esteban M A, Meseguer J. Effect of high dietary intake of vitamin C on non-specific immune response of gilthead seabream (Sparus aurata L.)[J]. Fish & Shellfish Immunology, 1999, 9(5):429-443.

[25] Fukuwatari T, Kurata K, Shibata K. Effects of excess nicotinic acid on growth and the urinary excretion of B-group vitamins and the metabolism of tryptophan in weaning rats[J]. Journal of the Food Hygienic Society of Japan, 2009, 50(2):80-84.

[26] Cooper D L, Murrell D E, Roane D S, et al. Effects of formulation design on niacin therapeutics:mechanism of action, metabolism, and drug delivery[J]. International Journal of Pharmaceutics, 2015, 490(1):55-64.

[27] Anderson J, Jackson A J, Matty A J, et al. Effects of dietary carbohydrate and fibre on the tilapia Oreochromis niloticus (Linn.)[J]. Aquaculture, 1984, 37(4):303-314.

[28] Shiau S Y, Kwok C C, Chen C J, et al. Effects of dietary fibre on the intestinal absorption of dextrin, blood sugar level and growth of tilapia, Oreochromis niloticus×O. aureus[J]. Journal of Fish Biology, 2010, 34(6):929-935.

[29] Lauring B, Taggart A K P, Tata J R, et al. Niacin lipid efficacy is independent of both the niacin receptor GPR109A and free fatty acid suppression[J]. Science Translational Medicine, 2012, 4(148):148ra115.

[30] Wang T J. Effect of niacin on dietary requirement and stress reponse ability of juvenile blunt snout bream, Megalobrama amblycephala[D]. Nanjing:Nanjing Argricultural University, 2014.[王天娇. 团头鲂幼鱼对烟酸的适宜需要量及调节抗应激能力的研究[D]. 南京:南京农业大学, 2014.]

[31] Carlson L A, Oro L. The effect of nicotinic acid on the plasma free fatty acid demonstration of a metabolic type of sympathicolysis[J]. Acta Medica Scandinavica, 1962, 172(6):641-645.

[32] Boonanuntanasarn S, Kumkhong S, Yoohat K, et al. Molecular responses of Nile tilapia (Oreochromis niloticus) to different levels of dietary carbohydrates[J]. Aquaculture, 2018, 482:117-123.

[33] Tian J, Wu F, Yang C G, et al. Dietary lipid levels impact lipoprotein lipase, hormone-sensitive lipase, and fatty acid synthetase gene expression in three tissues of adult GIFT strain of Nile tilapia, Oreochromis niloticus[J]. Fish Physiology and Biochemistry, 2015, 41(1):1-18.

[34] Chen G L, Liu L W, Xie S, et al. High-density lipoprotein metabolism and its influence to coronary heart disease[J]. Advances in Cardiovascular Diseases, 2010, 31(3):360-363.[陈国良, 刘立伟, 谢爽, 等. 高密度脂蛋白胆固醇代谢及其对冠心病影响的研究进展[J]. 心血管病学进展, 2010, 31(3):360-363.]

[35] Zhao S P, Dong J, Ni Z L, et al. Promotion of cholesterol reverse transport in mice in vivo by niacin[J]. Journal of Clinical Research, 2008, 25(9):1588-1590.[赵水平, 董静, 倪占玲, 等. 烟酸促进小鼠体内胆固醇逆转运[J]. 医学临床研究, 2008, 25(9):1588-1590.]

[36] Kamanna V S, Ganji S H, Kashyap M L. Recent advances in niacin and lipid metabolism[J]. Current Opinion in Lipidology, 2013, 24(3):239-245.

[37] Chen J, Jiang Y A. Effects of niacin on lipid metabolism in rat model of non-alcoholic fatty liver disease[J]. Journal of Clinical Hepatology, 2015, 31(2):261-265.[陈静, 江应安. 烟酸对非酒精性脂肪性肝病大鼠模型脂质代谢的影响[J]. 临床肝胆病杂志, 2015, 31(2):261-265.]

[38] Ganji S H, Kashyap M L, Kamanna V S. Niacin inhibits fat accumulation, oxidative stress, and inflammatory cytokine IL-8 in cultured hepatocytes:Impact on non-alcoholic fatty liver disease[J]. Metabolism, 2015, 64(9):982-990.

[39] Wang Y W, Zhang J L, Jiao J G, et al. Physiological and metabolic differences between visceral and subcutaneous adipose tissues in Nile tilapia (Oreochromis niloticus)[J]. American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 2017, 313(5):R608-R619.

[40] Ding L Y, Zhang L M, Wang J Y, et al. Effect of dietary lipid level on the growth performance, feed utilization, body composition and blood chemistry of juvenile starry flounder (Platichthys stellatus)[J]. Aquaculture Research, 2010, 41(10):1470-1478.