doi:

DOI: 10.3724/SP.J.1118.2019.18206

Journal of Fishery Sciences of China (中国水产科学) 2019/26:3 PP.577-585

Effects of Acanthosentis cheni infection on microbiota composition and diversity in the intestine of Coilia nasus


Abstract:
Coilia nasus samples were collected during the migration flood season of 2017. After dissection, six samples of C. nasus infected with Acanthosentis cheni were selected as the infected group, and six samples that were uninfected were selected as the control group to determine the effect of A. cheni infection on the intestinal microbial community structure of C. nasus. A total of twenty-three bacterial phyla were identified. Twenty-two phyla were identified in the control group and eighteen phyla were identified in the infected group, among which Verrucomicrobia was only detected in the infected group. The main bacteria phyla in the intestinal tract of C. nasus were Proteobacteria, Firmicutes, and Tenericutes, with a total relative abundance of more than 85%. The average content of Firmicutes, Fusobacteria, and Fibrobacteres in the infected group was higher than that in the control group, and the relative abundance of other bacteria phyla was lower than that in the control group, but there was no significant difference between the two groups (P > 0.05). Comparison of the relative abundance of bacteria at the family level showed that the proportions of Clostridiaceae, Peptostreptococcaceae, Lactobacillaceae from Firmicutes, and Enterobacteriaceae in the Proteobacteria were higher than those in the control group. Abundance of Peptostreptococcaceae, Lactobacillaceae, and Enterobacteriaceae in the 130 families identified were higher than those in the control group. Porphyromonadaceae and Eisenbergiella were significantly more abundant in the intestinal tract of the infected group (P < 0.05). At the community diversity level, there was no significant difference in Shannon index, Chao1 index, and Simpson index between the two groups (P > 0.05). Although the effects of A. cheni infection on the community structure and species diversity of intestinal microorganisms were not significant, there were significant differences in the composition of specific species.

Key words:Coilia nasus; Acanthosentis cheni; 16S rDNA; intestinal microbiota

ReleaseDate:2019-07-04 08:50:32



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