DOI: 10.3724/SP.J.1238.2013.00636

Journal of Hunan Agrichltural University (湖南农业大学学报) 2013/39:6 PP.636-640

Amplification and analysis of up-stream regulatory sequence of C–reactive protein gene (CRP) from grass carp (Ctenopharyngodon idellus)

C–reactive protein (CRP) is a kind of acute phase serum protein associated with innate immunity and inflammatory reaction of fish. A 1 055 bp DNA fragment from grass carp CRP gene up-stream sequence was amplified by Genome Walker. Sequence analyzing by online software PLACE and BDGP for prediction of promoter sequence and transtription factor binding sites indicated that the conserved initiate sequence of grass carp CRP gene is TCAGATC and the initiate site of transcription is G.RNA polymerase II binding site, TATA–Box, is located in –41 to –35 bp which embed in a highly conserved core promoter sequence (–54 to –5 bp), CAAT–Box appears in –94 to –91 bp. Moreover, some transcriptional factor binding sites related to inducible regulation of transcription are found in up–stream sequence, including 4 E–box elements, 3 GT1 consensus sequences, 2 GT1–core elements, and 2 I–box–core elements.

Key words:grass carp,C–reactive protein,up–stream regulatory sequence,transcription

ReleaseDate:2014-07-24 09:32:55