DOI: 10.3724/SP.J.1077.2009.00264

Journal of Inorganic Materials (无机材料学报) 2009/24:2 PP.264-268

Study on Preparation of n-HA and Silk Fibroin Bio-mineral Material

A new hydroxyapatite (n-HA)-Silk fibroin (SF) bio-mineral material was prepared by using a biomimetic method, with Ca(NO3)2 and Na3PO4 used as the starting reagent to synthesize the inorganic phase. In details, SF was dissolved in Ca(NO3)2 solution without desalting procedure, the compound solution was directly dropped into Na3PO4 solution. In this way, HA was synthesized at 37℃ by controlling Ca/P ratio, meanwhile the minerialized HA interacted with SF to form biomineral material. The morphology and structure of this n-HA-SF biomineral material were characterized by XRD, FTIR, XPS and SEM. The results show that there are strong chemical interaction between n-HA and SF. Moreover, in the biomineral material, the inorganic phase is calcium-deficient hydroxyapatite, containing a small amount of carbonated HA with an average crystal size of 37.6nm. In addition, The particle size of biomineral materials range from 50nm to 200nm.It can be deduced that silk fibroin chain can regulate the growth and morphology of HA crystals. The compressive strength of the biomineral material is 32.21MPa, which is a good potential material for bone tissue engineering and unloaded-bearing bone defects repairing.

Key words:biomineralization,nano-hydroxyapatite,silk fibroin,biomimetic synthesis

ReleaseDate:2014-07-21 14:31:39

[1]Du C, Cui F Z, Zhang W, et al. Journal of Biomedical Materials Research, 2000,50(4): 518-527.

[2]Bonfield W.Nature,1977,270(1):453-454.

[3]李玉宝.生物医学材料,第一版.北京:化学工业出版社, 2003:27-28.

[4]Wang H N, Li Y B, Zuo Y, et al. Biomaterials,2007,28(22): 3338-3348.

[5]Wang Li, Li Chun zhong. Carbohydrate Polymers,2007,68(4):740-745.

[6]Isamu Yamaguchi, Shunsuke Iizuka, Akiyoshi Osaka, et al. Colloids and Surfaces,2003,214(1-3):111-118.

[7]Rebecca L Horan, Kathryn Antle, Adam L Collette,et al. Biomaterials, 2005,26(17):3385-3393.

[8]姚菊明,魏克民,励 丽, 等. 化学学报,2007,65(7):635-639.

[9]黄志良,刘 羽,王大伟,等.材料导报,2002,16(10):69-71.

[10]Gregory H Altman, Frank Diaz, Caroline Jakuba, et al. Biomaterials,2003,24(3):401-416.

[11]Eun S Gil, David J Frankowski, Samuel M Hudson, et al. Materials Science and Engineering: C,2007,27(3):426-431.

[12]Kong X D, Cui F Z,Wang X M,et al.Journal of Crystal Growth,2004,270(1-2):197-202.

[13]杨 辉,张 林,张 宏,等.复合材料学报,2007,24(3):142-146.


[15]Pe′rez-Rigueiro J, Viney C, Llorca J, et al.Journal of Applied Polymer Science,2000, 75(10):1270-1277.

[16]李贵阳,周 平,孙尧俊,等.高等学校化学学报,2001,22(5):860-862.

[17]Wilson Donna,Valluzzi Regina, Kaplan David, et al. Biophysical Journal,2000,78(5): 2690-2701.

[18]Sonoyama M, Nakano T.Applied Spectroscopy,2000,54(7):968-973

[19]黄志良,王大伟,刘 羽.无机化学学报, 2002,5(18):469-474.

[20]王学江, 汪建新, 李玉宝, 等.高技术通讯,2000,10(11):92-94

[21]廖建国,王学江,左 奕,等(LIAO Jian-Guo,et al).无机材料学报(Journal of Inorganic Materials),2008,23(1):146-149.

[22]大连理工大学无机化学教研室编. 无机化学(第三版). 北京: 高等教育出版社,1990:4.

[23]肖 斌,周大利,杨为中,等(XIAO Bin,et al).无机材料学报(Journal of Inorganic Materials),2006,21(2):427-432.