Journal of Inorganic Materials (无机材料学报) 2014/29:7 PP.776-780
Synthetic calcium phosphate (CaP) has been investigated as promising biomaterial due to its excellent biocompatibility. CaP nanosheet-assembled microspheres were prepared using Ca(CH3COO)2, NaH2PO4·2H2O and PLA-mPEG in aqueous solution by microwave-assisted hydrothermal method at 120℃ for 30 min. Meanwhile, CaP polyhedral were prepared using Ca(CH3COO)2, NaH2PO4·2H2O and PLA-mPEG in mixed solvent of water and ethylene glycol (EG) by microwave-assisted solvothermal method at 120℃ for 30 min. The products were characterized with X-ray powder diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM) and thermogravimetric analysis (TG). EG showed a significant effect on the chemical composition and morphology of the product. The hemoglobin protein adsorption properties of the as-prepared products were investigated. The Hb adsorption amount increased with increasing Hb initial concentration, and were decreased with increasing volume of EG.
 FRATZL P, GUPTA H S, PASCHALIS E P, et al. Structure and mechanical quality of the collagen-mineral nano-composite in bone. J. Mater. Chem., 2004, 14(14): 2115-2123.
 TZAPHLIDOU M. Bone architecture: collagen structure and calcium/ phosphorus maps. J. Biol. Phys., 2008, 34(1/2): 39-49.
 ONG J L, CHAN D C N. Hydroxyapatite and their use as coatings in dental implants: a review. Crit. Rev. Biomed. Eng., 2000, 28(5/6): 667a-707a.
 YANG S B,WANG J, LIU C S. Research on calcium phosphate cement bone adhesive. J. Inorg. Mater., 2013, 28(1): 85-90.
 CHEN X Q, CHEN X N, ZHU X D, et al. Effect of surface topography of hydroxyapatite on human osteosarcoma MG-63 cell. J. Inorg. Mater., 2013, 28(8): 901-906.
 LAI C, TANG S Q, WANG Y J, et al. Formation of calcium phosphate nanoparticles in reverse microemulsions. Mater. Lett., 2005, 59(2/3): 210-214.
 LIN KL, CHANG J, ZHU Y J, et al. A facile one-step surfactant-free and low-temperature hydrothermal method to prepare uniform 3D structured carbonated apatite flowers. Cryst. Growth Des., 2009, 9(1): 177-181.
 CHEN F, ZHU Y J, WANG K W, et al. Surfactant-free solvothermal synthesis of hydroxyapatite nanowire/nanotube ordered arrays with biomimetic structures. CrystEngComm, 2011, 13(6): 1858-1863.
 CHEN F, TANG Q L, ZHU Y J, et al. Hydroxyapatite nanorods/poly(vinyl pyrolidone) composite nanofibers, arrays and three-dimensional fabrics: Electrospun preparation and transformation to hydroxyapatite nanostructures. Acta Biomater., 2010, 6(8): 3013-3020.
 CHEN X, TANG Q L, ZHU Y J, et al. Synthesis and antibacterial property of zinc loaded hydroxyapatite nanorods. Mater. Lett., 2012, 89: 233-235.
 LUO H T, ZHI W, LU X, et al. Research on preparation and biological properties of dense hydroxyapatite spheres. J. Inorg. Mater., 2013, 28(1): 40-44.
 CHEN F, ZHU Y J, WU J, et al. Nanostructured calcium phosphates: preparation and their application in biomedicine. Nano. Biomed. Eng., 2012, 4(1): 41-49.
 WANG K F, ZHOU C C, HONG Y L, et al. Review article: a review of protein adsorption on bioceramics. Interface Focus, 2012, 2(3): 259-277.
 ZENG H, CHITTUR K K, LACEFIELD W R. Analysis of bovine serum albumin adsorption on calcium phosphate and titanium surfaces. Biomaterials, 1999, 20(4): 377-384.
 WASSELL D T H, HALL R C, EMBERY G. Adsorption of bovine serum albumin onto hydroxyapatite. Biomaterials, 1995, 16(9): 697-702.
 BAGHBANZADEH M, CARBONE L, COZZOLI P D, et al. Microwave-assisted synthesis of colloidal inorganic nanocrystals. Angew. Chem. Int. Edit., 2011, 50(48): 11312-11359.
 GEDYE R, SMITH F, WESTAWAY K, et al. The use of microwave-ovens for rapid organic-synthesis. Tetrahedron Lett., 1986, 27(3): 279-282.
 GIGUERE R J, BRAY T L, DUNCAN S M, et al. Application of commercial microwave-ovens to organic-synthesis. Tetrahedron Lett., 1986, 27(41): 4945-4948.
 ZHU Y J, WANG W W, QI R J, et al. Microwave-assisted synthesis of single-crystalline tellurium nanorods and nanowires in ionic liquids. Angew. Chem. Int. Edit., 2004, 43(11): 1410-1414.
 ZHAO J, ZHU Y J, ZHENG J Q, et al. Microwave-assisted hydrothermal preparation using adenosine 5'-triphosphate disodium salt as a phosphate source and characterization of zinc-doped amorphous calcium phosphate mesoporous microspheres. Micropor. Mesopor. Mater., 2013, 180: 79-85.
 ZHAO X Y, ZHU Y J, QI C, et al. Hierarchical hollow hydroxyapatite microspheres: microwave-assisted rapid synthesis by using pyridoxal-5 '-phosphate as a phosphorus source and application in drug delivery. Chem. Asian J., 2013, 8(6): 1313-1320.
 QI C, ZHU Y J, LU B Q, CHEN F, et al. Hydroxyapatite hierarchically nanostructured porous hollow microspheres: rapid, sustainable microwave-hydrothermal synthesis by using creatine phosphate as an organic phosphorus source and application in drug delivery and protein adsorption. Chem. Eur. J., 2013, 19(17): 5332-5341.
 QI C, ZHU Y J, ZHAO X Y, et al. Highly stable amorphous calcium phosphate porous nanospheres: microwave-assisted rapid synthesis using ATP as phosphorus source and stabilizer, and their application in anticancer drug delivery. Chem. Eur. J., 2013, 19(3): 981-987.
 CHEN F, HUANG P, ZHU Y J, et al. The photoluminescence, drug delivery and imaging properties of multifunctional Eu3+/Gd3+ dual-doped hydroxyapatite nanorods. Biomaterials, 2011, 32(34): 9031-9039.
 WANG K W, ZHU Y J, CHEN X Y, et al. Flower-like hierarchically nanostructured hydroxyapatite hollow spheres: facile preparation and application in anticancer drug cellular delivery. Chem. Asian J., 2010, 5(12): 2477-2482.