DOI: 10.3724/SP.J.1249.2016.01001

Journal of Shenzhen University Science and Engineering (深圳大学学报理工版) 2016/33:1 PP.1-9

Experiments on biocompatibility of PEEK-SEEK-HA composite materials with U2OS cells

Effects of polyetheretherketone-hydroxyapatite (PEEK-HA) composites and polyetheretherketone-sulfnated polyetheretherketone-hydroxyapatite (PEEK-SPEEK-HA) composites on U2OS cells are studied by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet razolium bromide (MTT) method. It is found that cell proliferation in PEEK-SPEEK-HA composites is broader than that in PEEK-HA composites. With an increase of HA content in PEEK-HA composites, cell proliferation accelerates. The material of sulfnated polyetheretherketone promotes cell proliferation significantly at 4-8 mg/mL concentration. A cytotoxicity test shows that these bio-materials are non-toxic to U2OS cells and the U2OS cells can adapt to these materials. With an increase of HA content, the cell adhesion rate increases and is higher in PEEK-SPEEK-HA composites than in PEEK-HA composites, which indicates that adding HA and SPEEK in PEEK composite could improve the adhesion property. Cell apoptosis test by the flow cytometry (FCM) indicates that the addition of SPEEK in PEEK-HA composites can inhibit the early apoptosis thus decreasing the apoptosis rate. A test on the effect of SPEEK on the reactive oxygen species (ROS) of U2OS cells by using the flow cytometry (FCM) and the confocal laser scanning microscopy (CLSM) shows that SPEEK in PEEK-HA composites can decrease the ROS level, and reduce the cytotoxicity. Scanning electron microscope (SEM) results show that U2OS cells can adhere, stretch and grow on surfaces of PEEK-SPEEK-HA composites.

Key words:polyetheretherketone-hydroxyapatite (PEEK-HA) composite,U2OS cell,cell morphology,proliferation,cell adhesion,cell apoptosis,reactive oxygen species (ROS)

ReleaseDate:2016-04-11 16:08:17

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